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Yorodumi- PDB-1eku: CRYSTAL STRUCTURE OF A BIOLOGICALLY ACTIVE SINGLE CHAIN MUTANT OF... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1eku | ||||||
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Title | CRYSTAL STRUCTURE OF A BIOLOGICALLY ACTIVE SINGLE CHAIN MUTANT OF HUMAN IFN-GAMMA | ||||||
Components | Interferon gamma | ||||||
Keywords | IMMUNE SYSTEM / CYTOKINE / PROTEIN ENGINEERING / INTERFERON | ||||||
Function / homology | Function and homology information positive regulation of fructose 1,6-bisphosphate metabolic process / positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity / positive regulation of tumor necrosis factor (ligand) superfamily member 11 production / positive regulation of iron ion import across plasma membrane / type II interferon receptor binding / positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation involved in immune response / negative regulation of tau-protein kinase activity / positive regulation of NMDA glutamate receptor activity / : / positive regulation of vitamin D biosynthetic process ...positive regulation of fructose 1,6-bisphosphate metabolic process / positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity / positive regulation of tumor necrosis factor (ligand) superfamily member 11 production / positive regulation of iron ion import across plasma membrane / type II interferon receptor binding / positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation involved in immune response / negative regulation of tau-protein kinase activity / positive regulation of NMDA glutamate receptor activity / : / positive regulation of vitamin D biosynthetic process / positive regulation of cellular respiration / positive regulation of peptidyl-serine phosphorylation of STAT protein / positive regulation of interleukin-23 production / negative regulation of amyloid-beta clearance / positive regulation of calcidiol 1-monooxygenase activity / positive regulation of protein deacetylation / type III interferon-mediated signaling pathway / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / positive regulation of smooth muscle cell apoptotic process / positive regulation of core promoter binding / neuroinflammatory response / positive regulation of exosomal secretion / positive regulation of killing of cells of another organism / macrophage activation involved in immune response / positive regulation of osteoclast differentiation / negative regulation of interleukin-17 production / positive regulation of MHC class II biosynthetic process / positive regulation of signaling receptor activity / positive regulation of membrane protein ectodomain proteolysis / positive regulation of neurogenesis / negative regulation of epithelial cell differentiation / positive regulation of amyloid-beta formation / IFNG signaling activates MAPKs / positive regulation of epithelial cell migration / positive regulation of nitric-oxide synthase biosynthetic process / cell surface receptor signaling pathway via JAK-STAT / regulation of insulin secretion / humoral immune response / macrophage differentiation / positive regulation of autophagy / Regulation of IFNG signaling / type II interferon-mediated signaling pathway / extrinsic apoptotic signaling pathway / positive regulation of chemokine production / positive regulation of phagocytosis / positive regulation of tyrosine phosphorylation of STAT protein / positive regulation of interleukin-12 production / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / positive regulation of glycolytic process / positive regulation of interleukin-1 beta production / positive regulation of cytokine production / cytokine activity / astrocyte activation / negative regulation of smooth muscle cell proliferation / positive regulation of protein localization to plasma membrane / microglial cell activation / positive regulation of protein-containing complex assembly / response to virus / positive regulation of protein serine/threonine kinase activity / cellular response to virus / positive regulation of protein import into nucleus / positive regulation of inflammatory response / positive regulation of interleukin-6 production / positive regulation of tumor necrosis factor production / Interferon gamma signaling / positive regulation of nitric oxide biosynthetic process / defense response to virus / adaptive immune response / cell surface receptor signaling pathway / negative regulation of gene expression / negative regulation of DNA-templated transcription / apoptotic process / positive regulation of cell population proliferation / positive regulation of gene expression / negative regulation of transcription by RNA polymerase II / extracellular space / extracellular region Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.9 Å | ||||||
Authors | Landar, A. / Curry, B. / Parker, M.H. / DiGiacomo, R. / Indelicato, S.R. / Walter, M.R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2000 Title: Design, characterization, and structure of a biologically active single-chain mutant of human IFN-gamma. Authors: Landar, A. / Curry, B. / Parker, M.H. / DiGiacomo, R. / Indelicato, S.R. / Nagabhushan, T.L. / Rizzi, G. / Walter, M.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1eku.cif.gz | 113.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1eku.ent.gz | 90 KB | Display | PDB format |
PDBx/mmJSON format | 1eku.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1eku_validation.pdf.gz | 453.7 KB | Display | wwPDB validaton report |
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Full document | 1eku_full_validation.pdf.gz | 477.4 KB | Display | |
Data in XML | 1eku_validation.xml.gz | 21.9 KB | Display | |
Data in CIF | 1eku_validation.cif.gz | 28.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ek/1eku ftp://data.pdbj.org/pub/pdb/validation_reports/ek/1eku | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 31173.404 Da / Num. of mol.: 2 / Mutation: H111D, L121F Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Cell line: T-CELLS, NK CELLS / Gene: IFNG / Production host: Escherichia coli (E. coli) / References: UniProt: P01579 #2: Chemical | ChemComp-SO4 / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.99 Å3/Da / Density % sol: 58.81 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 1.4 M NaCitrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 63 % | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS / Detector: IMAGE PLATE / Date: Nov 12, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→20 Å / Num. all: 16757 / Num. obs: 16757 / % possible obs: 99.9 % / Observed criterion σ(F): -3 / Observed criterion σ(I): -3 / Redundancy: 10.9 % / Biso Wilson estimate: 85 Å2 / Rmerge(I) obs: 0.065 / Net I/σ(I): 25.1 |
Reflection shell | Resolution: 2.9→3 Å / Redundancy: 10 % / Rmerge(I) obs: 0.6 / Num. unique all: 1659 / % possible all: 100 |
Reflection | *PLUS Num. measured all: 182491 |
-Processing
Software |
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Refinement | Resolution: 2.9→20 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.9→20 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.4 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.9 Å / Lowest resolution: 20 Å / σ(F): 0 / Rfactor obs: 0.246 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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