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- PDB-1ebu: HOMOSERINE DEHYDROGENASE COMPLEX WITH NAD ANALOGUE AND L-HOMOSERINE -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ebu | |||||||||
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Title | HOMOSERINE DEHYDROGENASE COMPLEX WITH NAD ANALOGUE AND L-HOMOSERINE | |||||||||
![]() | HOMOSERINE DEHYDROGENASE | |||||||||
![]() | OXIDOREDUCTASE / homoserine / dehydrogenase / dinucleotide / ternary / analogue | |||||||||
Function / homology | ![]() aspartate family amino acid biosynthetic process / aspartate kinase activity / homoserine dehydrogenase / homoserine dehydrogenase activity / homoserine biosynthetic process / threonine biosynthetic process / methionine biosynthetic process / isoleucine biosynthetic process / NADP binding / nucleus / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() | |||||||||
![]() | DeLaBarre, B. / Thompson, P.R. / Wright, G.D. / Berghuis, A.M. | |||||||||
![]() | ![]() Title: Crystal structures of homoserine dehydrogenase suggest a novel catalytic mechanism for oxidoreductases. Authors: DeLaBarre, B. / Thompson, P.R. / Wright, G.D. / Berghuis, A.M. #1: ![]() Title: Crystallization and preliminary X-ray diffraction studies of homoserine dehydrogenase from Saccharomyces cerevisiae Authors: DeLaBarre, B. / Jacques, S.L. / Pratt, C.E. / Wright, G.D. / Berghuis, A.M. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 282.2 KB | Display | ![]() |
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PDB format | ![]() | 229.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 788.5 KB | Display | ![]() |
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Full document | ![]() | 834.3 KB | Display | |
Data in XML | ![]() | 59 KB | Display | |
Data in CIF | ![]() | 80.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | The asymmetric unit contains 2 dimers: A/B and C/D. The ternary complex is found in the D chain protomer |
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Components
#1: Protein | Mass: 38413.766 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: HOM6P / Production host: ![]() ![]() #2: Chemical | ChemComp-NA / #3: Chemical | ChemComp-NDA / | #4: Chemical | ChemComp-HSE / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.74 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: PEG 8K, L-homoserine, 3-aminopyridine dinucleotide, sodium cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 8.5 | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 218 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: Feb 22, 1996 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→40 Å / Num. all: 44409 / Num. obs: 164716 / % possible obs: 99.2 % / Observed criterion σ(F): 3 / Observed criterion σ(I): 0 / Redundancy: 3.7 % / Biso Wilson estimate: 35.4 Å2 / Rmerge(I) obs: 0.081 / Net I/σ(I): 13.3 |
Reflection shell | Resolution: 2.6→2.69 Å / Redundancy: 3.5 % / Rmerge(I) obs: 0.344 / Num. unique all: 4392 / % possible all: 98.1 |
Reflection | *PLUS |
Reflection shell | *PLUS % possible obs: 98.1 % |
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Processing
Software |
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Refinement | Resolution: 2.6→39.4 Å / Rfactor Rfree error: 0.004 / Data cutoff high absF: 1625863.12 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 / σ(I): 0 / Stereochemistry target values: Engh & Huber / Details: Model refined against amplitudes
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 34 Å2 / ksol: 0.322 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.6 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.6→39.4 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.6→2.76 Å / Rfactor Rfree error: 0.016 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.9 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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