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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1e9h | ||||||
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タイトル | Thr 160 phosphorylated CDK2 - Human cyclin A3 complex with the inhibitor indirubin-5-sulphonate bound | ||||||
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![]() | CELL CYCLE / COMPLEX (PROTEIN KINASE-CYCLIN) / KINASES / INHIBITOR / CYCLIN / COMPLEX / PHOSPHORYLATION | ||||||
機能・相同性 | ![]() Phosphorylation of proteins involved in the G2/M transition by Cyclin A:Cdc2 complexes / cyclin A2-CDK1 complex / cell cycle G1/S phase transition / cellular response to luteinizing hormone stimulus / mitotic cell cycle phase transition / Transcription of E2F targets under negative control by p107 (RBL1) and p130 (RBL2) in complex with HDAC1 / cellular response to leptin stimulus / male pronucleus / female pronucleus / cellular response to cocaine ...Phosphorylation of proteins involved in the G2/M transition by Cyclin A:Cdc2 complexes / cyclin A2-CDK1 complex / cell cycle G1/S phase transition / cellular response to luteinizing hormone stimulus / mitotic cell cycle phase transition / Transcription of E2F targets under negative control by p107 (RBL1) and p130 (RBL2) in complex with HDAC1 / cellular response to leptin stimulus / male pronucleus / female pronucleus / cellular response to cocaine / response to glucagon / cyclin-dependent protein serine/threonine kinase regulator activity / cellular response to insulin-like growth factor stimulus / positive regulation of DNA biosynthetic process / cochlea development / cyclin A1-CDK2 complex / cyclin E2-CDK2 complex / cyclin E1-CDK2 complex / cellular response to platelet-derived growth factor stimulus / cyclin A2-CDK2 complex / positive regulation of DNA-templated DNA replication initiation / G2 Phase / cyclin-dependent protein kinase activity / Y chromosome / Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes / positive regulation of heterochromatin formation / p53-Dependent G1 DNA Damage Response / X chromosome / PTK6 Regulates Cell Cycle / regulation of DNA replication / regulation of anaphase-promoting complex-dependent catabolic process / Defective binding of RB1 mutants to E2F1,(E2F2, E2F3) / centriole replication / Regulation of APC/C activators between G1/S and early anaphase / centrosome duplication / Telomere Extension By Telomerase / G0 and Early G1 / Activation of the pre-replicative complex / cellular response to nitric oxide / cyclin-dependent protein kinase holoenzyme complex / cyclin-dependent kinase / animal organ regeneration / cyclin-dependent protein serine/threonine kinase activity / TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest / Activation of ATR in response to replication stress / Cyclin E associated events during G1/S transition / Cyclin A/B1/B2 associated events during G2/M transition / Cajal body / Cyclin A:Cdk2-associated events at S phase entry / condensed chromosome / mitotic G1 DNA damage checkpoint signaling / regulation of mitotic cell cycle / regulation of G2/M transition of mitotic cell cycle / cyclin binding / post-translational protein modification / : / meiotic cell cycle / positive regulation of DNA replication / male germ cell nucleus / cellular response to estradiol stimulus / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / potassium ion transport / DNA Damage/Telomere Stress Induced Senescence / CDK-mediated phosphorylation and removal of Cdc6 / Meiotic recombination / SCF(Skp2)-mediated degradation of p27/p21 / Orc1 removal from chromatin / G1/S transition of mitotic cell cycle / Transcriptional regulation of granulopoiesis / G2/M transition of mitotic cell cycle / Cyclin D associated events in G1 / cellular senescence / positive regulation of fibroblast proliferation / Regulation of TP53 Degradation / nuclear envelope / Factors involved in megakaryocyte development and platelet production / Processing of DNA double-strand break ends / cellular response to hypoxia / Senescence-Associated Secretory Phenotype (SASP) / regulation of gene expression / peptidyl-serine phosphorylation / DNA replication / Regulation of TP53 Activity through Phosphorylation / transcription regulator complex / Ras protein signal transduction / chromosome, telomeric region / Ub-specific processing proteases / endosome / chromatin remodeling / protein domain specific binding / cell division / protein phosphorylation / DNA repair / protein serine kinase activity / protein serine/threonine kinase activity / DNA-templated transcription / centrosome / positive regulation of cell population proliferation / protein kinase binding / positive regulation of DNA-templated transcription 類似検索 - 分子機能 | ||||||
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手法 | ![]() ![]() ![]() | ||||||
![]() | Davies, T.G. / Tunnah, P. / Noble, M.E.M. / Endicott, J.A. | ||||||
![]() | ![]() タイトル: Inhibitor Binding to Active and Inactive Cdk2: The Crystal Structure of Cdk2-Cyclin A/Indirubin-5-Sulphonate 著者: Davies, T.G. / Tunnah, P. / Meijer, L. / Marko, D. / Eisenbrand, G. / Endicott, J.A. / Noble, M.E. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 238.2 KB | 表示 | ![]() |
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PDB形式 | ![]() | 192.1 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 555.4 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 607.6 KB | 表示 | |
XML形式データ | ![]() | 30.2 KB | 表示 | |
CIF形式データ | ![]() | 45.7 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | |
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類似構造データ |
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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非結晶学的対称性 (NCS) | NCS oper: (Code: given Matrix: (-0.999932, -0.011239, 0.003138), ベクター: |
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要素
#1: タンパク質 | 分子量: 33873.195 Da / 分子数: 2 / 由来タイプ: 組換発現 詳細: PHOSPHORYLATED ON THR 160 CHAINS A AND C ARE ASYMETRIC UNIT COPIES 由来: (組換発現) ![]() ![]() ![]() #2: タンパク質 | 分子量: 29964.713 Da / 分子数: 2 / 由来タイプ: 組換発現 詳細: TRUNCATED FRAGMENT OF CYCLIN A. IN COMPLEX WITH CDK2 CHAINS B AND D ARE ASYMETRIC UNIT COPIES 由来: (組換発現) ![]() ![]() ![]() #3: 化合物 | #4: 水 | ChemComp-HOH / | 構成要素の詳細 | THE CDK2 SHOWS MAXIMAL ACTIVITY DURING S PHASE AND G2 AND INTERACTS WITH CYCLINS A, D, OR E. IT IS ...THE CDK2 SHOWS MAXIMAL ACTIVITY DURING S PHASE AND G2 AND INTERACTS WITH CYCLINS A, D, OR E. IT IS PROBABLY INVOLVED IN THE CONTROL OF THE CELL CYCLE. CYCLIN IS ESSENTIAL FOR THE CONTROL OF THE CELL CYCLE AT THE G1/S(START) AND THE G2/M (MITOSIS) TRANSITION | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.91 Å3/Da / 溶媒含有率: 57.71 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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結晶化 | pH: 7 / 詳細: pH 7.00 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS 手法: 蒸気拡散法, ハンギングドロップ法 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | 日付: 2000年4月15日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.933 Å / 相対比: 1 |
反射 | 解像度: 2.5→20 Å / Num. obs: 47127 / % possible obs: 90.1 % / 冗長度: 3 % / Rmerge(I) obs: 0.109 / Net I/σ(I): 4.1 |
反射 シェル | 解像度: 2.5→2.59 Å / 冗長度: 2.3 % / Rmerge(I) obs: 0.39 / Mean I/σ(I) obs: 1.9 / % possible all: 90.1 |
反射 | *PLUS 最高解像度: 2.5 Å / 最低解像度: 20 Å / Num. measured all: 143005 / Rmerge(I) obs: 0.103 |
反射 シェル | *PLUS % possible obs: 63.8 % / Rmerge(I) obs: 0.388 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: UNPUBLISHED STRUCTURE 解像度: 2.5→100 Å / 交差検証法: THROUGHOUT / σ(F): 0
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精密化ステップ | サイクル: LAST / 解像度: 2.5→100 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: CNS / バージョン: 1 / 分類: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS 最低解像度: 100 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS |