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Open data
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Basic information
Entry | Database: PDB / ID: 1.0E+96 | ||||||
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Title | Structure of the Rac/p67phox complex | ||||||
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Function / homology | ![]() superoxide-generating NAD(P)H oxidase activity / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lapouge, K. / Smith, S.J.M. / Walker, P.A. / Gamblin, S.J. / Smerdon, S.J. / Rittinger, K. | ||||||
![]() | ![]() Title: Structure of the TPR domain of p67phox in complex with Rac.GTP. Authors: Lapouge, K. / Smith, S.J. / Walker, P.A. / Gamblin, S.J. / Smerdon, S.J. / Rittinger, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 83.9 KB | Display | ![]() |
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PDB format | ![]() | 66.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 322.4 KB | Display | ![]() |
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Full document | ![]() | 331.3 KB | Display | |
Data in XML | ![]() | 10.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 21429.062 Da / Num. of mol.: 1 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 23490.471 Da / Num. of mol.: 1 / Fragment: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
#3: Chemical | ChemComp-GTP / ![]() |
#4: Chemical | ChemComp-MG / |
#5: Water | ChemComp-HOH / ![]() |
Compound details | CHAIN A ENGINEERED MUTATION MET1PRO, GLN61LEU RAC1 ARE GTP-BINDING PROTEINS ASSOCIATED WITH PLASMA ...CHAIN A ENGINEERED |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.08 Å3/Da / Density % sol: 54.6 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | pH: 10.5 Details: 0.1 M CAPS PH 10.5, 0.2 M LI ACETATE, 0.8 M NAH2PO4, 1.2 M K2HPO4 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / pH: 7.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Apr 15, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.4→15 Å / Num. obs: 123873 / % possible obs: 94 % / Redundancy: 5.9 % / Rmerge(I) obs: 0.084 / Net I/σ(I): 16.9 |
Reflection shell | Resolution: 2.4→2.45 Å / Rmerge(I) obs: 0.45 / Mean I/σ(I) obs: 4.2 / % possible all: 100 |
Reflection | *PLUS Num. obs: 20975 / Num. measured all: 123873 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 1MH1, 1A17 Resolution: 2.4→15 Å / SU ML: 0.189 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.411 / ESU R Free: 0.288
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Displacement parameters | Biso mean: 29.7 Å2
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Refinement step | Cycle: LAST / Resolution: 2.4→15 Å
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Refine LS restraints |
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