+Open data
-Basic information
Entry | Database: PDB / ID: 1dov | ||||||
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Title | CRYSTAL STRUCTURE OF THE ALPHA-CATENIN DIMERIZATION DOMAIN | ||||||
Components | ALPHA-CATENINAlpha catenin | ||||||
Keywords | CELL ADHESION / four-helix bundle | ||||||
Function / homology | Function and homology information negative regulation of integrin-mediated signaling pathway / VEGFR2 mediated vascular permeability / RHO GTPases activate IQGAPs / Adherens junctions interactions / gap junction assembly / epithelial cell-cell adhesion / zonula adherens / gamma-catenin binding / vinculin binding / cellular response to indole-3-methanol ...negative regulation of integrin-mediated signaling pathway / VEGFR2 mediated vascular permeability / RHO GTPases activate IQGAPs / Adherens junctions interactions / gap junction assembly / epithelial cell-cell adhesion / zonula adherens / gamma-catenin binding / vinculin binding / cellular response to indole-3-methanol / flotillin complex / negative regulation of cell motility / Myogenesis / apical junction assembly / positive regulation of extrinsic apoptotic signaling pathway in absence of ligand / positive regulation of smoothened signaling pathway / catenin complex / negative regulation of protein localization to nucleus / axon regeneration / negative regulation of neuroblast proliferation / smoothened signaling pathway / establishment or maintenance of cell polarity / odontogenesis of dentin-containing tooth / neuroblast proliferation / negative regulation of extrinsic apoptotic signaling pathway in absence of ligand / intercalated disc / ovarian follicle development / extrinsic apoptotic signaling pathway in absence of ligand / acrosomal vesicle / cell motility / integrin-mediated signaling pathway / adherens junction / protein localization / cell-cell adhesion / beta-catenin binding / response to estrogen / male gonad development / actin filament binding / cell-cell junction / cell migration / actin cytoskeleton / lamellipodium / cell junction / regulation of cell population proliferation / cadherin binding / intracellular membrane-bounded organelle / apoptotic process / protein-containing complex binding / structural molecule activity / negative regulation of apoptotic process / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 3 Å | ||||||
Authors | Pokutta, S. / Weis, W.I. | ||||||
Citation | Journal: Mol.Cell / Year: 2000 Title: Structure of the dimerization and beta-catenin-binding region of alpha-catenin. Authors: Pokutta, S. / Weis, W.I. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1dov.cif.gz | 41.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1dov.ent.gz | 30.6 KB | Display | PDB format |
PDBx/mmJSON format | 1dov.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/do/1dov ftp://data.pdbj.org/pub/pdb/validation_reports/do/1dov | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Details | The biological assembly is a dimer. The twofold axis of the dimer is coincident with a crystallographic twofold. The dimer can by generated by application of the following rotation matrix: (-0.5 0.866 0.0) (0.866 0.5 0.0) (0.0 0.0 -1.0) and the translation vector (0. 0. 118.17) |
-Components
#1: Protein | Mass: 19875.953 Da / Num. of mol.: 1 / Fragment: DIMERIZATION DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Production host: Escherichia coli (E. coli) / References: UniProt: P26231 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.33 Å3/Da / Density % sol: 76.91 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 8.9 Details: PEG 400, Tris, urea, dithiothreitol, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 288 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL9-1 / Wavelength: 0.98 |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Nov 25, 1997 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 3→40 Å / Num. all: 33971 / Num. obs: 8574 / % possible obs: 99.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 3 / Redundancy: 4 % / Rmerge(I) obs: 0.082 / Net I/σ(I): 11.6 |
Reflection shell | Resolution: 3→3.16 Å / Redundancy: 4 % / Rmerge(I) obs: 0.322 / Num. unique all: 5031 / % possible all: 100 |
Reflection | *PLUS Num. measured all: 33971 |
Reflection shell | *PLUS % possible obs: 100 % / Num. measured obs: 5031 / Mean I/σ(I) obs: 4.1 |
-Processing
Software |
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Refinement | Resolution: 3→40 Å / Rfactor Rfree error: 0.009 / Data cutoff high absF: 2593339.74 / Data cutoff low absF: 0 / Isotropic thermal model: GROUP / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: ENGH AND HUBER
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 60.52 Å2 / ksol: 0.326 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 69.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3→40 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3→3.11 Å / Rfactor Rfree error: 0.04 / Total num. of bins used: 10
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Xplor file | Serial no: 1 / Param file: PROTEIN_REP.PARAM / Topol file: PROTEIN.TOP | |||||||||||||||||||||||||
Software | *PLUS Name: CNS / Version: 0.5 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Num. reflection obs: 7667 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 66.9 Å2 | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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