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Open data
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Basic information
Entry | Database: PDB / ID: 1dad | ||||||||||||||||||||||||
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Title | DETHIOBIOTIN SYNTHETASE COMPLEXED WITH ADP | ||||||||||||||||||||||||
![]() | DETHIOBIOTIN SYNTHETASE | ||||||||||||||||||||||||
![]() | ![]() ![]() | ||||||||||||||||||||||||
Function / homology | Dethiobiotin synthase BioD / P-loop containing nucleoside triphosphate hydrolase / CobQ/CobB/MinD/ParA nucleotide binding domain / ![]() ![]() ![]() ![]() ![]() | ||||||||||||||||||||||||
Specimen source | ![]() ![]() ![]() | ||||||||||||||||||||||||
Method | ![]() | ||||||||||||||||||||||||
![]() | Huang, W. / Jia, J. / Schneider, G. / Lindqvist, Y. | ||||||||||||||||||||||||
![]() | Journal: Biochemistry / Year: 1995 Title: Mechanism of an ATP-dependent carboxylase, dethiobiotin synthetase, based on crystallographic studies of complexes with substrates and a reaction intermediate. ![]() #1: Journal: Structure / Year: 1994 Title: Crystal Structure of an ATP-Dependent Carboxylase, Dethiobiotin Synthetase, at 1.65 A Resolution ![]() | ||||||||||||||||||||||||
Validation Report | ![]() ![]() ![]() | ||||||||||||||||||||||||
Date | Deposition: May 8, 1995 / Release: Jun 20, 1996
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmcif format | ![]() ![]() |
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PDB format | ![]() ![]() |
PDBML Plus | ![]() |
Others | ![]() |
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Links
-Related structure data
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell | γ α β
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Components
#1: Protein/peptide | Mass: 24028.289 Da / Num. of mol.: 1 / Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-ADP / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.21 / Density percent sol: 34 % | ||||||||||||||||||||||||||||||
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Crystal grow![]() | *PLUS Temp: 20 ℃ / pH: 6.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
components of the solutions | *PLUS
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-Data collection
Source | Wavelength: 1.5418 |
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Detector | Type: RIGAKU / Detector: IMAGE PLATE / Collection date: May 3, 1994 |
Radiation | Monochromatic or laue m l: M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Number obs: 25367 / Observed criterion sigma I: 1 / Rmerge I obs: 0.051 / Redundancy: 3.6 % / Percent possible obs: 87 |
Reflection | *PLUS D resolution high: 1.6 Å / Number measured all: 91245 |
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Processing
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Refine | Sigma F: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | B iso mean: 27.1 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||
Least-squares process | R factor R work![]() | |||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine hist #LAST | Highest resolution: 1.6 Å / Lowest resolution: 6 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||
Number of atoms included #LAST | Protein: 1692 / Nucleic acid: 0 / Ligand: 27 / Solvent: 139 / Total: 1858 | |||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
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