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Yorodumi- PDB-1cz5: NMR STRUCTURE OF VAT-N: THE N-TERMINAL DOMAIN OF VAT (VCP-LIKE AT... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1cz5 | ||||||
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Title | NMR STRUCTURE OF VAT-N: THE N-TERMINAL DOMAIN OF VAT (VCP-LIKE ATPASE OF THERMOPLASMA) | ||||||
Components | VCP-LIKE ATPASE | ||||||
Keywords | HYDROLASE / DOUBLE-PSI BETA-BARREL / BETA-CLAM / SUBSTRATE RECOGNITION DOMAIN / FUSION PROTEIN | ||||||
Function / homology | Function and homology information macromolecule metabolic process / primary metabolic process / response to stimulus / : / ATP hydrolysis activity / ATP binding Similarity search - Function | ||||||
Biological species | Thermoplasma acidophilum (acidophilic) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Coles, M. / Diercks, T. / Liermann, J. / Groeger, A. / Rockel, B. / Baumeister, W. / Koretke, K. / Lupas, A. / Peters, J. / Kessler, H. | ||||||
Citation | Journal: Curr.Biol. / Year: 1999 Title: The solution structure of VAT-N reveals a 'missing link' in the evolution of complex enzymes from a simple betaalphabetabeta element. Authors: Coles, M. / Diercks, T. / Liermann, J. / Groger, A. / Rockel, B. / Baumeister, W. / Koretke, K.K. / Lupas, A. / Peters, J. / Kessler, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1cz5.cif.gz | 1.4 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1cz5.ent.gz | 1.2 MB | Display | PDB format |
PDBx/mmJSON format | 1cz5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cz/1cz5 ftp://data.pdbj.org/pub/pdb/validation_reports/cz/1cz5 | HTTPS FTP |
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-Related structure data
Related structure data | 1cz4C C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 20649.816 Da / Num. of mol.: 1 Fragment: N-TERMINAL DOMAIN: M1 TO E183 FOLLOWED BY A DIGLYCINE SPACER Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermoplasma acidophilum (acidophilic) / Plasmid: PET28 / Production host: Escherichia coli (E. coli) / References: UniProt: O05209 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY. 3JHNHA COUPLING-CONSTANTS FROM AN HNHA SPECTRUM |
-Sample preparation
Details |
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Sample conditions |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: STRUCTURES BASED ON 1923 DISTANCE RESTRAINTS; 1817 NOE BASED RESTRAINTS, 196 DIHEDRAL ANGLE RESTRAINTS, 56 J-COUPLING RESTRAINTS, 106 DISTANCE RESTRAINTS FOR H-BONDS, 158 CA AND 148 CB ...Details: STRUCTURES BASED ON 1923 DISTANCE RESTRAINTS; 1817 NOE BASED RESTRAINTS, 196 DIHEDRAL ANGLE RESTRAINTS, 56 J-COUPLING RESTRAINTS, 106 DISTANCE RESTRAINTS FOR H-BONDS, 158 CA AND 148 CB CHEMICAL SHIFT RESTRAINTS. REFINED WITH A CONFORMATIONAL DATABASE POTENTIAL | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 50 / Conformers submitted total number: 25 |