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基本情報
登録情報 | データベース: PDB / ID: 1cxz | ||||||
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タイトル | CRYSTAL STRUCTURE OF HUMAN RHOA COMPLEXED WITH THE EFFECTOR DOMAIN OF THE PROTEIN KINASE PKN/PRK1 | ||||||
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![]() | SIGNALING PROTEIN / PROTEIN-PROTEIN COMPLEX / ANTIPARALLEL COILED-COIL | ||||||
機能・相同性 | ![]() epithelial cell migration / protein kinase C / diacylglycerol-dependent serine/threonine kinase activity / alpha-beta T cell lineage commitment / aortic valve formation / mitotic cleavage furrow formation / positive regulation of lipase activity / bone trabecula morphogenesis / endothelial tube lumen extension / skeletal muscle satellite cell migration ...epithelial cell migration / protein kinase C / diacylglycerol-dependent serine/threonine kinase activity / alpha-beta T cell lineage commitment / aortic valve formation / mitotic cleavage furrow formation / positive regulation of lipase activity / bone trabecula morphogenesis / endothelial tube lumen extension / skeletal muscle satellite cell migration / positive regulation of vascular associated smooth muscle contraction / angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure / renal system process / SLIT2:ROBO1 increases RHOA activity / RHO GTPases Activate Rhotekin and Rhophilins / Roundabout signaling pathway / negative regulation of intracellular steroid hormone receptor signaling pathway / Axonal growth inhibition (RHOA activation) / Axonal growth stimulation / cleavage furrow formation / regulation of neural precursor cell proliferation / regulation of modification of postsynaptic actin cytoskeleton / regulation of osteoblast proliferation / forebrain radial glial cell differentiation / regulation of cell motility / regulation of modification of postsynaptic structure / apical junction assembly / cell junction assembly / negative regulation of cell migration involved in sprouting angiogenesis / cellular response to chemokine / establishment of epithelial cell apical/basal polarity / beta selection / regulation of systemic arterial blood pressure by endothelin / B cell apoptotic process / negative regulation of oxidative phosphorylation / negative regulation of motor neuron apoptotic process / RHO GTPases Activate ROCKs / negative regulation of cell size / regulation of germinal center formation / RHO GTPases activate CIT / Sema4D induced cell migration and growth-cone collapse / PCP/CE pathway / regulation of immunoglobulin production / RHO GTPases activate KTN1 / regulation of androgen receptor signaling pathway / positive regulation of podosome assembly / apolipoprotein A-I-mediated signaling pathway / positive regulation of alpha-beta T cell differentiation / Sema4D mediated inhibition of cell attachment and migration / hyperosmotic response / wound healing, spreading of cells / positive regulation of leukocyte adhesion to vascular endothelial cell / PI3K/AKT activation / Wnt signaling pathway, planar cell polarity pathway / motor neuron apoptotic process / odontogenesis / ossification involved in bone maturation / regulation of focal adhesion assembly / negative chemotaxis / nuclear androgen receptor binding / apical junction complex / RHOB GTPase cycle / EPHA-mediated growth cone collapse / myosin binding / negative regulation of B cell proliferation / stress fiber assembly / positive regulation of cytokinesis / regulation of neuron projection development / RHOC GTPase cycle / cellular response to cytokine stimulus / cerebral cortex cell migration / ERBB2 Regulates Cell Motility / cleavage furrow / semaphorin-plexin signaling pathway / B cell homeostasis / androgen receptor signaling pathway / ficolin-1-rich granule membrane / RHOA GTPase cycle / mitotic spindle assembly / negative regulation of cell-substrate adhesion / Rho protein signal transduction / positive regulation of protein serine/threonine kinase activity / positive regulation of T cell migration / endothelial cell migration / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / skeletal muscle tissue development / RHO GTPases activate PKNs / GPVI-mediated activation cascade / spleen development / positive regulation of stress fiber assembly / negative regulation of reactive oxygen species biosynthetic process / cytoplasmic microtubule organization / EPHB-mediated forward signaling / RAC1 GTPase cycle / substantia nigra development / positive regulation of neuron differentiation / substrate adhesion-dependent cell spreading / post-translational protein modification / regulation of microtubule cytoskeleton organization / protein kinase C binding 類似検索 - 分子機能 | ||||||
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![]() | Maesaki, R. / Ihara, K. / Shimizu, T. / Kuroda, S. / Kaibuchi, K. / Hakoshima, T. | ||||||
![]() | ![]() タイトル: The structural basis of Rho effector recognition revealed by the crystal structure of human RhoA complexed with the effector domain of PKN/PRK1. 著者: Maesaki, R. / Ihara, K. / Shimizu, T. / Kuroda, S. / Kaibuchi, K. / Hakoshima, T. #1: ![]() タイトル: Biochemical and crystallographic characterization of a Rho effector domain of the protein serine/threonine kinase N in a complex with RhoA 著者: Maesaki, R. / Shimizu, T. / Ihara, K. / Kuroda, S. / Kaibuchi, K. / Hakoshima, T. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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PDBx/mmCIF形式 | ![]() | 69.9 KB | 表示 | ![]() |
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PDB形式 | ![]() | 50.9 KB | 表示 | ![]() |
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その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 733.8 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 735.5 KB | 表示 | |
XML形式データ | ![]() | 13.1 KB | 表示 | |
CIF形式データ | ![]() | 17.8 KB | 表示 | |
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-関連構造データ
関連構造データ | |
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 20596.615 Da / 分子数: 1 / 断片: RESIDUES 1 - 181 / 変異: G14V / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
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#2: タンパク質 | 分子量: 9811.257 Da / 分子数: 1 / 断片: RESIDUES 13 - 98 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
#3: 化合物 | ChemComp-MG / |
#4: 化合物 | ChemComp-GSP / |
#5: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.75 Å3/Da / 溶媒含有率: 44 % | |||||||||||||||||||||||||
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結晶化 | 温度: 277 K / 手法: マイクロバッチ法 / pH: 6.5 / 詳細: PEG 300, pH 6.5, MICROBATCH, temperature 277K | |||||||||||||||||||||||||
結晶化 | *PLUS 温度: 4 ℃ / 手法: batch method / 詳細: Maesaki, R., (1999) J.Struct.Biol., 126, 166. | |||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 90 K |
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放射光源 | 由来: ![]() |
検出器 | タイプ: RIGAKU RAXIS IV++ / 検出器: IMAGE PLATE / 日付: 1999年1月5日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | 解像度: 2.2→49.8 Å / Num. all: 696854 / Num. obs: 17568 / % possible obs: 96 % / Observed criterion σ(I): 1 / Biso Wilson estimate: 32.8 Å2 / Rmerge(I) obs: 0.09 / Net I/σ(I): 24 |
反射 シェル | 解像度: 2.2→2.28 Å / Rmerge(I) obs: 0.312 / % possible all: 86 |
反射 | *PLUS % possible obs: 96 % / Num. measured all: 696854 / Rmerge(I) obs: 0.09 |
反射 シェル | *PLUS 最高解像度: 2.2 Å / % possible obs: 86 % / Mean I/σ(I) obs: 2.8 |
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解析
ソフトウェア |
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精密化 | 解像度: 2.2→49.8 Å / σ(F): 2 / 立体化学のターゲット値: PROTEIN.PARAM
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Refine analyze |
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精密化ステップ | サイクル: LAST / 解像度: 2.2→49.8 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: CNS / 分類: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS 最高解像度: 2.2 Å / 最低解像度: 49.8 Å / σ(F): 2 / % reflection Rfree: 5 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS タイプ: c_dihedral_angle_deg / Dev ideal: 22.45 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS精密化 シェル | *PLUS 最高解像度: 2.2 Å / 最低解像度: 2.28 Å / Rfactor Rfree: 0.359 / Rfactor obs: 0.315 |