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Yorodumi- PDB-1cto: NMR STRUCTURE OF THE C-TERMINAL DOMAIN OF THE LIGAND-BINDING REGI... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1cto | ||||||
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Title | NMR STRUCTURE OF THE C-TERMINAL DOMAIN OF THE LIGAND-BINDING REGION OF MURINE GRANULOCYTE COLONY-STIMULATING FACTOR RECEPTOR, MINIMIZED AVERAGE STRUCTURE | ||||||
Components | GRANULOCYTE COLONY-STIMULATING FACTOR RECEPTOR | ||||||
Keywords | BINDING PROTEIN / CYTOKINE RECEPTOR | ||||||
Function / homology | Function and homology information granulocyte colony-stimulating factor binding / regulation of myeloid cell differentiation / amelogenesis / cytokine receptor activity / immunoglobulin mediated immune response / neutrophil chemotaxis / cytokine-mediated signaling pathway / endocytic vesicle membrane / cell adhesion / external side of plasma membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / RANDOM.INP, DGSA.INP, REFINE.INP | ||||||
Authors | Yamasaki, K. / Naito, S. / Anaguchi, H. / Ohkubo, T. / Ota, Y. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1997 Title: Solution structure of an extracellular domain containing the WSxWS motif of the granulocyte colony-stimulating factor receptor and its interaction with ligand. Authors: Yamasaki, K. / Naito, S. / Anaguchi, H. / Ohkubo, T. / Ota, Y. #1: Journal: J.Biol.Chem. / Year: 1995 Title: Ligand Binding Characteristics of the Carboxyl-Terminal Domain of the Cytokine Receptor Homologous Region of the Granulocyte Colony-Stimulating Factor Receptor Authors: Anaguchi, H. / Hiraoka, O. / Yamasaki, K. / Naito, S. / Ota, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1cto.cif.gz | 42.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1cto.ent.gz | 32.7 KB | Display | PDB format |
PDBx/mmJSON format | 1cto.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1cto_validation.pdf.gz | 340.2 KB | Display | wwPDB validaton report |
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Full document | 1cto_full_validation.pdf.gz | 354.4 KB | Display | |
Data in XML | 1cto_validation.xml.gz | 5.6 KB | Display | |
Data in CIF | 1cto_validation.cif.gz | 7.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ct/1cto ftp://data.pdbj.org/pub/pdb/validation_reports/ct/1cto | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12536.452 Da / Num. of mol.: 1 / Fragment: C-TERMINAL DOMAIN OF THE LIGAND-BINDING REGION / Mutation: INS(G1,S2,S3) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) Description: EXPRESSED AS A FUSION PROTEIN WITH MALTOSE BINDING PROTEIN Plasmid: PMAL-P / Production host: Escherichia coli (E. coli) / References: UniProt: P40223 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | pH: 5.5 / Temperature: 303 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker AMX600 / Manufacturer: Bruker / Model: AMX600 / Field strength: 600 MHz |
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-Processing
Software |
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NMR software |
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Refinement | Method: RANDOM.INP, DGSA.INP, REFINE.INP / Software ordinal: 1 | ||||||||||||
NMR ensemble | Conformer selection criteria: MINIMIZED AVERAGE / Conformers calculated total number: 100 / Conformers submitted total number: 1 |