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Yorodumi- PDB-1crb: CRYSTALLOGRAPHIC STUDIES ON A FAMILY OF CELLULAR LIPOPHILIC TRANS... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1crb | ||||||
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Title | CRYSTALLOGRAPHIC STUDIES ON A FAMILY OF CELLULAR LIPOPHILIC TRANSPORT PROTEINS. REFINEMENT OF P2 MYELIN PROTEIN AND THE STRUCTURE DETERMINATION AND REFINEMENT OF CELLULAR RETINOL-BINDING PROTEIN IN COMPLEX WITH ALL-TRANS-RETINOL | ||||||
Components | CELLULAR RETINOL BINDING PROTEIN | ||||||
Keywords | CELLULAR LIPOPHILIC TRANSPORT PROTEIN | ||||||
Function / homology | Function and homology information regulation of granulocyte differentiation / response to benzoic acid / The canonical retinoid cycle in rods (twilight vision) / Retinoid metabolism and transport / all-trans-retinol binding / retinoic acid biosynthetic process / vitamin A metabolic process / retinal binding / response to vitamin A / retinol metabolic process ...regulation of granulocyte differentiation / response to benzoic acid / The canonical retinoid cycle in rods (twilight vision) / Retinoid metabolism and transport / all-trans-retinol binding / retinoic acid biosynthetic process / vitamin A metabolic process / retinal binding / response to vitamin A / retinol metabolic process / retinoic acid metabolic process / retinol binding / lipid homeostasis / fatty acid transport / response to retinoic acid / lipid droplet / fatty acid binding / cell body / nucleoplasm / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Rattus rattus (black rat) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.1 Å | ||||||
Authors | Cowan, S.W. / Jones, T.A. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1993 Title: Crystallographic studies on a family of cellular lipophilic transport proteins. Refinement of P2 myelin protein and the structure determination and refinement of cellular retinol-binding ...Title: Crystallographic studies on a family of cellular lipophilic transport proteins. Refinement of P2 myelin protein and the structure determination and refinement of cellular retinol-binding protein in complex with all-trans-retinol. Authors: Cowan, S.W. / Newcomer, M.E. / Jones, T.A. #1: Journal: Adv.Protein Chem. / Year: 1994 Title: Lipid-Binding Proteins: A Family of Fatty Acid and Retinoid Transport Proteins Authors: Banaszak, L. / Winter, N. / Xu, Z. / Bernlohr, D.A. / Cowan, S. / Jones, T.A. #2: Journal: Eur.J.Biochem. / Year: 1992 Title: The Primary Structure of Piscine (Oncorhynchus Mykiss) Retinol-Binding Protein and a Comparison with the Three-Dimensional Structure of Mammalian Retinol-Binding Protein Authors: Zapponi, M.C. / Zanotti, G. / Stoppini, M. / Berni, R. #3: Journal: Proteins / Year: 1990 Title: Crystallographic Refinement of Human Serum Retinol Binding Protein at 2 Angstroms Resolution Authors: Cowan, S.W. / Newcomer, M.E. / Jones, T.A. #4: Journal: J.Biol.Chem. / Year: 1981 Title: Crystallization of and Preliminary X-Ray Data for an Intracellular Vitamin A-Binding Protein from Rat Liver Authors: Newcomer, M.E. / Liljas, A. / Eriksson, V. / Sundelin, J. / Rask, L. / Peterson, P.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1crb.cif.gz | 41.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1crb.ent.gz | 28.4 KB | Display | PDB format |
PDBx/mmJSON format | 1crb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1crb_validation.pdf.gz | 597.9 KB | Display | wwPDB validaton report |
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Full document | 1crb_full_validation.pdf.gz | 598 KB | Display | |
Data in XML | 1crb_validation.xml.gz | 8 KB | Display | |
Data in CIF | 1crb_validation.cif.gz | 10.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cr/1crb ftp://data.pdbj.org/pub/pdb/validation_reports/cr/1crb | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 15724.871 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus rattus (black rat) / References: UniProt: P02696 | ||||
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#2: Chemical | #3: Chemical | ChemComp-RTL / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.09 Å3/Da / Density % sol: 41.26 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 4 ℃ / pH: 8 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.1 Å / % possible obs: 80 % |
-Processing
Software |
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Refinement | Resolution: 2.1→8 Å / Rfactor Rfree: 0.248 / Rfactor Rwork: 0.188 / Rfactor obs: 0.188 / σ(F): 0 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.188 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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