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- PDB-1cis: CONTEXT DEPENDENCE OF PROTEIN SECONDARY STRUCTURE FORMATION. THE ... -

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Entry
Database: PDB / ID: 1cis
TitleCONTEXT DEPENDENCE OF PROTEIN SECONDARY STRUCTURE FORMATION. THE THREE-DIMENSIONAL STRUCTURE AND STABILITY OF A HYBRID BETWEEN CHYMOTRYPSIN INHIBITOR 2 AND HELIX E FROM SUBTILISIN CARLSBERG
ComponentsHYBRID PROTEIN FORMED FROM CHYMOTRYPSIN INHIBITOR-2
KeywordsHYBRID PROTEIN
Function / homology
Function and homology information


serine-type endopeptidase inhibitor activity / response to wounding
Similarity search - Function
Trypsin Inhibitor V, subunit A / Proteinase inhibitor I13, potato inhibitor I / Proteinase inhibitor I13, potato inhibitor I superfamily / Potato inhibitor I family / Potato inhibitor I family signature. / Trypsin Inhibitor V; Chain A / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Subtilisin-chymotrypsin inhibitor-2A
Similarity search - Component
Biological speciesHordeum vulgare (barley)
Bacillus licheniformis (bacteria)
MethodSOLUTION NMR
AuthorsOsmark, P. / Sorensen, P. / Poulsen, F.M.
Citation
Journal: Biochemistry / Year: 1993
Title: Context dependence of protein secondary structure formation: the three-dimensional structure and stability of a hybrid between chymotrypsin inhibitor 2 and helix E from subtilisin Carlsberg.
Authors: Osmark, P. / Sorensen, P. / Poulsen, F.M.
#1: Journal: J.Mol.Biol. / Year: 1991
Title: Refinement of the Three-Dimensional Solution Structure of Barley Serine Proteinase Inhibitor 2 and Comparison with the Structures in Crystals
Authors: Ludvigsen, S. / Shen, H. / Kjaer, M. / Madsen, J.C. / Poulsen, F.M.
#2: Journal: Eur.J.Biochem. / Year: 1987
Title: The High-Resolution X-Ray Crystal Structure of the Complex Formed between Subtilisin Carlsberg and Eglin C, an Elastase Inhibitor from the Leech Hirudo Medicinalis: Structural Analysis, ...Title: The High-Resolution X-Ray Crystal Structure of the Complex Formed between Subtilisin Carlsberg and Eglin C, an Elastase Inhibitor from the Leech Hirudo Medicinalis: Structural Analysis, Subtilisin Structure and Interface Geometry
Authors: Bode, W. / Papamokos, E. / Musil, D.
History
DepositionApr 23, 1993Processing site: BNL
Revision 1.0Oct 31, 1993Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Nov 29, 2017Group: Derived calculations / Other
Category: pdbx_database_status / pdbx_struct_assembly ...pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_conf / struct_conf_type
Item: _pdbx_database_status.process_site
Revision 1.4Apr 10, 2024Group: Data collection / Database references
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ref_seq_dif.details

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Assembly

Deposited unit
A: HYBRID PROTEIN FORMED FROM CHYMOTRYPSIN INHIBITOR-2


Theoretical massNumber of molelcules
Total (without water)7,5301
Polymers7,5301
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)15 / -
Representative

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Components

#1: Protein HYBRID PROTEIN FORMED FROM CHYMOTRYPSIN INHIBITOR-2


Mass: 7529.688 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Hordeum vulgare, Bacillus licheniformis
Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P01053

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Crystal grow
*PLUS
Method: other / Details: NMR

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Processing

Software
NameClassification
X-PLORmodel building
X-PLORrefinement
X-PLORphasing
NMR software
NameVersionDeveloperClassification
DIANA2GUNTERT,BRAUN,WUTHRICHrefinement
X-PLOR3BRUNGERrefinement
NMR ensembleConformers submitted total number: 15

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