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- PDB-1bu3: REFINED CRYSTAL STRUCTURE OF CALCIUM-BOUND SILVER HAKE (PI 4.2) P... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1bu3 | ||||||
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Title | REFINED CRYSTAL STRUCTURE OF CALCIUM-BOUND SILVER HAKE (PI 4.2) PARVALBUMIN AT 1.65 A. | ||||||
![]() | CALCIUM-BINDING PROTEIN | ||||||
![]() | CALCIUM BINDING | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Richardson, R.C. / Nelson, D.J. / Royer, W.E. / Harrington, D.J. | ||||||
![]() | ![]() Title: X-Ray crystal structure and molecular dynamics simulations of silver hake parvalbumin (Isoform B). Authors: Richardson, R.C. / King, N.M. / Harrington, D.J. / Sun, H. / Royer, W.E. / Nelson, D.J. #1: ![]() Title: Isolation of an Unusual Parvalbumin from the White Muscle of the Silver Hake (Merluccius Bilinearis) Authors: Zhang, C.Y. / Speno, H. / Clairmont, C. / Nelson, D.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 31.5 KB | Display | ![]() |
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PDB format | ![]() | 23 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5cpvS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 11354.724 Da / Num. of mol.: 1 / Fragment: CALCIUM-BINDING DOMAIN / Source method: isolated from a natural source / Source: (natural) ![]() | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 51 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 6 / Details: pH 6.00 | ||||||||||||||||||||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, sitting drop / PH range low: 6.5 / PH range high: 5.5 | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Details: MIRRORS |
Radiation | Monochromator: NI FILTER / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.65→8 Å / Num. obs: 15685 / % possible obs: 99.9 % / Observed criterion σ(I): 2 / Redundancy: 6 % / Biso Wilson estimate: 19 Å2 / Rsym value: 0.071 |
Reflection | *PLUS Num. measured all: 129847 / Rmerge(I) obs: 0.071 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: 5CPV Resolution: 1.65→8 Å / Cross valid method: THROUGHOUT / σ(F): 2
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Displacement parameters | Biso mean: 16.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.25 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.65→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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