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- PDB-1bte: CRYSTAL STRUCTURE OF THE EXTRACELLULAR DOMAIN OF THE TYPE II ACTI... -

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Basic information

Entry
Database: PDB / ID: 1bte
TitleCRYSTAL STRUCTURE OF THE EXTRACELLULAR DOMAIN OF THE TYPE II ACTIVIN RECEPTOR
ComponentsPROTEIN (ACTIVIN RECEPTOR TYPE II)
KeywordsTRANSFERASE / RECEPTOR / SERINE KINASE / LIGAND BINDING DOMAIN / THREE-FINGER TOXIN
Function / homology
Function and homology information


penile erection / TGFBR3 regulates activin signaling / Signaling by Activin / Sertoli cell proliferation / inhibin binding / inhibin-betaglycan-ActRII complex / activin receptor activity / activin receptor activity, type II / positive regulation of activin receptor signaling pathway / Signaling by BMP ...penile erection / TGFBR3 regulates activin signaling / Signaling by Activin / Sertoli cell proliferation / inhibin binding / inhibin-betaglycan-ActRII complex / activin receptor activity / activin receptor activity, type II / positive regulation of activin receptor signaling pathway / Signaling by BMP / positive regulation of follicle-stimulating hormone secretion / gastrulation with mouth forming second / embryonic skeletal system development / sperm ejaculation / cellular response to oxygen-glucose deprivation / BMP receptor activity / pattern specification process / activin receptor activity, type I / activin receptor complex / anterior/posterior pattern specification / SMAD protein signal transduction / receptor protein serine/threonine kinase / cellular response to BMP stimulus / activin binding / determination of left/right symmetry / activin receptor signaling pathway / negative regulation of ossification / odontogenesis of dentin-containing tooth / regulation of nitric oxide biosynthetic process / positive regulation of bone mineralization / growth factor binding / mesoderm development / positive regulation of osteoblast differentiation / positive regulation of SMAD protein signal transduction / BMP signaling pathway / regulation of signal transduction / coreceptor activity / positive regulation of erythrocyte differentiation / male gonad development / PDZ domain binding / cellular response to growth factor stimulus / positive regulation of protein phosphorylation / osteoblast differentiation / autophagy / spermatogenesis / signaling receptor complex / protein serine/threonine kinase activity / cell surface / positive regulation of transcription by RNA polymerase II / ATP binding / metal ion binding / identical protein binding / plasma membrane / cytoplasm
Similarity search - Function
CD59 / CD59 / Ser/Thr protein kinase, TGFB receptor / Snake toxin-like superfamily / Ribbon / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase domain profile. ...CD59 / CD59 / Ser/Thr protein kinase, TGFB receptor / Snake toxin-like superfamily / Ribbon / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / Mainly Beta
Similarity search - Domain/homology
Activin receptor type-2A
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MIRAS / Resolution: 1.5 Å
AuthorsGreenwald, J. / Fischer, W. / Vale, W. / Choe, S.
CitationJournal: Nat.Struct.Biol. / Year: 1999
Title: Three-finger toxin fold for the extracellular ligand-binding domain of the type II activin receptor serine kinase.
Authors: Greenwald, J. / Fischer, W.H. / Vale, W.W. / Choe, S.
History
DepositionSep 1, 1998Deposition site: BNL / Processing site: RCSB
Revision 1.0Feb 9, 1999Provider: repository / Type: Initial release
Revision 1.1Oct 16, 2007Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Non-polymer description / Version format compliance
Revision 2.0Dec 11, 2019Group: Advisory / Atomic model / Derived calculations
Category: atom_site / pdbx_struct_assembly ...atom_site / pdbx_struct_assembly / pdbx_struct_assembly_gen / pdbx_struct_special_symmetry / pdbx_validate_symm_contact / struct_conn
Item: _atom_site.occupancy / _struct_conn.pdbx_leaving_atom_flag
Revision 2.1Jul 29, 2020Group: Data collection / Derived calculations / Structure summary
Category: chem_comp / entity ...chem_comp / entity / pdbx_chem_comp_identifier / pdbx_entity_nonpoly / struct_conn / struct_site / struct_site_gen
Item: _chem_comp.name / _chem_comp.type ..._chem_comp.name / _chem_comp.type / _entity.pdbx_description / _pdbx_entity_nonpoly.name / _struct_conn.pdbx_role
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.2Oct 30, 2024Group: Data collection / Database references / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI ..._chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: PROTEIN (ACTIVIN RECEPTOR TYPE II)
B: PROTEIN (ACTIVIN RECEPTOR TYPE II)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)23,9006
Polymers23,0162
Non-polymers8854
Water3,639202
1
A: PROTEIN (ACTIVIN RECEPTOR TYPE II)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,9503
Polymers11,5081
Non-polymers4422
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
2
B: PROTEIN (ACTIVIN RECEPTOR TYPE II)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,9503
Polymers11,5081
Non-polymers4422
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)71.630, 71.630, 37.380
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number143
Space group name H-MP3
Components on special symmetry positions
IDModelComponents
11A-154-

HOH

21A-156-

HOH

31B-168-

HOH

Noncrystallographic symmetry (NCS)NCS oper: (Code: given
Matrix: (0.8945, -0.4458, -0.0349), (0.4445, 0.895, -0.0382), (0.0482, 0.0187, 0.9987)
Vector: -19.7137, -37.4157, -14.0347)

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Components

#1: Protein PROTEIN (ACTIVIN RECEPTOR TYPE II)


Mass: 11507.803 Da / Num. of mol.: 2 / Fragment: LIGAND-BINDING DOMAIN
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Plasmid: PPIC9K / Production host: Pichia pastoris (fungus) / Strain (production host): SMD1168
References: UniProt: P27038, Transferases; Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor
#2: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 202 / Source method: isolated from a natural source / Formula: H2O
Compound detailsGLYCOSYLATION FROM HOST EXPRESSION SYSTEM WAS REMOVED USING ENDOGLYCOSIDASE H, LEAVING BEHIND THE N- ...GLYCOSYLATION FROM HOST EXPRESSION SYSTEM WAS REMOVED USING ENDOGLYCOSIDASE H, LEAVING BEHIND THE N-ACETYL GLUCOSAMINE (NAG) RESIDUES ON THE ASN.
Has protein modificationY
Nonpolymer detailsO6 OF NAG A 124 AND NAG B 124 IS MODELED IN TWO ALTERNATIVE CONFORMATIONS.
Sequence detailsTHE NUMBERING OF RESIDUES IN THE PDB FILE IS BASED ON THE SEQUENCE WITHOUT THE SIGNAL PEPTIDE. THE ...THE NUMBERING OF RESIDUES IN THE PDB FILE IS BASED ON THE SEQUENCE WITHOUT THE SIGNAL PEPTIDE. THE MOLECULE IN THE CRYSTAL IS LACKING THE 14 C-TERMINAL RESIDUES WHICH HAVE BEEN REMOVED BY TREATMENT WITH ENDOPROTEINASE GLUC, MAKING GLU 102, THE NEW C-TERMINUS

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.4 Å3/Da / Density % sol: 42 %
Crystal growpH: 4.5
Details: 100 MM SODIUM ACETATE, PH 4.5, 5% PEG 8000, 0.5M NACL
Crystal
*PLUS
Density % sol: 43 %
Crystal grow
*PLUS
Method: unknown
Components of the solutions
*PLUS
IDConc.Common nameCrystal-IDSol-IDChemical formula
15 %PEG11
20.5 M11NaCl
30.1 M11NaOAc

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ALS / Beamline: 5.0.2 / Wavelength: 0.97
DetectorType: ADSC / Detector: CCD / Date: Jan 15, 1998
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97 Å / Relative weight: 1
ReflectionResolution: 1.5→50 Å / Num. obs: 30005 / % possible obs: 83.9 % / Redundancy: 4.2 % / Biso Wilson estimate: 18.1 Å2 / Rsym value: 0.034 / Net I/σ(I): 11.6
Reflection shellResolution: 1.5→1.54 Å / Redundancy: 2.9 % / Mean I/σ(I) obs: 0.3 / Rsym value: 0.295 / % possible all: 37.3
Reflection
*PLUS
Rmerge(I) obs: 0.034

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Processing

Software
NameVersionClassification
MOSFLMdata reduction
SCALAdata scaling
MLPHAREphasing
ARPmodel building
DMmodel building
REFMACrefinement
CCP4(SCALA)data scaling
ARP/wARPmodel building
DMphasing
RefinementMethod to determine structure: MIRAS / Resolution: 1.5→20 Å / SU ML: 0.063 / σ(F): 0 / ESU R: 0.086 / ESU R Free: 0.092
RfactorNum. reflection% reflectionSelection details
Rfree0.222 1500 5 %RANDOM
Rwork0.181 ---
obs-30005 83.9 %-
Displacement parametersBiso mean: 23.6 Å2
Refinement stepCycle: LAST / Resolution: 1.5→20 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms1484 0 56 202 1742
Refine LS restraints
Refine-IDTypeDev idealDev ideal target
X-RAY DIFFRACTIONp_bond_d0.0130.02
X-RAY DIFFRACTIONp_angle_d0.0290.04
X-RAY DIFFRACTIONp_angle_deg
X-RAY DIFFRACTIONp_planar_d0.0320.05
X-RAY DIFFRACTIONp_hb_or_metal_coord
X-RAY DIFFRACTIONp_mcbond_it1.6132
X-RAY DIFFRACTIONp_mcangle_it2.6783
X-RAY DIFFRACTIONp_scbond_it2.0072
X-RAY DIFFRACTIONp_scangle_it2.9893
X-RAY DIFFRACTIONp_plane_restr
X-RAY DIFFRACTIONp_chiral_restr0.1410.15
X-RAY DIFFRACTIONp_singtor_nbd0.1710.3
X-RAY DIFFRACTIONp_multtor_nbd0.2560.3
X-RAY DIFFRACTIONp_xhyhbond_nbd
X-RAY DIFFRACTIONp_xyhbond_nbd
X-RAY DIFFRACTIONp_planar_tor5.57
X-RAY DIFFRACTIONp_staggered_tor16.515
X-RAY DIFFRACTIONp_orthonormal_tor
X-RAY DIFFRACTIONp_transverse_tor20.620
X-RAY DIFFRACTIONp_special_tor
Software
*PLUS
Name: REFMAC / Classification: refinement
Refinement
*PLUS
Highest resolution: 1.5 Å / σ(F): 0 / % reflection Rfree: 5 % / Rfactor obs: 0.181
Solvent computation
*PLUS
Displacement parameters
*PLUS
Biso mean: 23.6 Å2
Refine LS restraints
*PLUS
Refine-IDTypeDev idealDev ideal target
X-RAY DIFFRACTIONp_bond_d0.0130.02
X-RAY DIFFRACTIONp_angle_d0.0290.04
X-RAY DIFFRACTIONp_planar_d0.0320.05
X-RAY DIFFRACTIONp_chiral_restr0.1410.15

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