+Open data
-Basic information
Entry | Database: PDB / ID: 1b9w | ||||||
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Title | C-TERMINAL MEROZOITE SURFACE PROTEIN 1 FROM PLASMODIUM CYNOMOLGI | ||||||
Components | PROTEIN (MEROZOITE SURFACE PROTEIN 1) | ||||||
Keywords | SURFACE PROTEIN / MSP-1 / CANDIDATE MALARIA VACCINE / SURFACE ANTIGEN | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Plasmodium cynomolgi (eukaryote) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SIRAS / Resolution: 1.8 Å | ||||||
Authors | Bentley, G.A. / Chitarra, V. / Holm, I. / Longacre, S. | ||||||
Citation | Journal: Mol.Cell / Year: 1999 Title: The crystal structure of C-terminal merozoite surface protein 1 at 1.8 A resolution, a highly protective malaria vaccine candidate. Authors: Chitarra, V. / Holm, I. / Bentley, G.A. / Petres, S. / Longacre, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1b9w.cif.gz | 30.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1b9w.ent.gz | 20.6 KB | Display | PDB format |
PDBx/mmJSON format | 1b9w.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1b9w_validation.pdf.gz | 421.9 KB | Display | wwPDB validaton report |
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Full document | 1b9w_full_validation.pdf.gz | 423.1 KB | Display | |
Data in XML | 1b9w_validation.xml.gz | 6.7 KB | Display | |
Data in CIF | 1b9w_validation.cif.gz | 8.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b9/1b9w ftp://data.pdbj.org/pub/pdb/validation_reports/b9/1b9w | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 10795.188 Da / Num. of mol.: 1 / Fragment: TWO C-TERMINAL EGF-LIKE DOMAINS Source method: isolated from a genetically manipulated source Source: (gene. exp.) Plasmodium cynomolgi (eukaryote) / Description: SYNTHETIC GENE / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q25659 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 40 % | ||||||||||||||||||||
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Crystal grow | pH: 5.5 / Details: pH 5.50 | ||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||
Crystal grow | *PLUS pH: 5.6 / Method: unknown | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: SYNCHROTRON / Site: LURE / Beamline: DW32 / Wavelength: 0.97 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Mar 1, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→15 Å / Num. obs: 9965 / % possible obs: 100 % / Redundancy: 13.9 % / Biso Wilson estimate: 20.7 Å2 / Rsym value: 0.058 |
Reflection | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 15 Å / % possible obs: 100 % / Redundancy: 13.9 % / Rmerge(I) obs: 0.058 / Biso Wilson estimate: 20.7 Å2 |
Reflection shell | *PLUS Rmerge(I) obs: 0.338 |
-Processing
Software |
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Refinement | Method to determine structure: SIRAS / Resolution: 1.8→15 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 27.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→15 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 15 Å / Rfactor obs: 0.212 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |