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Open data
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Basic information
Entry | Database: PDB / ID: 1boh | ||||||
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Title | SULFUR-SUBSTITUTED RHODANESE (ORTHORHOMBIC FORM) | ||||||
![]() | RHODANESE | ||||||
![]() | TRANSFERASE / RHODANESE / SULFURTRANSFERASE | ||||||
Function / homology | ![]() rRNA transport / 3-mercaptopyruvate sulfurtransferase activity / thiosulfate sulfurtransferase / thiosulfate sulfurtransferase activity / rRNA import into mitochondrion / 5S rRNA binding / mitochondrial matrix / mitochondrion Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Gliubich, F. / Berni, R. / Cianci, M. / Trevino, R.J. / Horowitz, P.M. / Zanotti, G. | ||||||
![]() | ![]() Title: NH2-terminal sequence truncation decreases the stability of bovine rhodanese, minimally perturbs its crystal structure, and enhances interaction with GroEL under native conditions. Authors: Trevino, R.J. / Gliubich, F. / Berni, R. / Cianci, M. / Chirgwin, J.M. / Zanotti, G. / Horowitz, P.M. #1: ![]() Title: Structure of Sulfur-Substituted Rhodanese at 1.36 A Resolution Authors: Gliubich, F. / Berni, R. / Colapietro, M. / Barba, L. / Zanotti, G. #2: ![]() Title: Active Site Structural Features for Chemically Modified Forms of Rhodanese Authors: Gliubich, F. / Gazerro, M. / Zanotti, G. / Delbono, S. / Bombieri, G. / Berni, R. #3: ![]() Title: The Structure of Bovine Liver Rhodanese. II. The Active Site in the Sulfur-Substituted and the Sulfur-Free Enzyme Authors: Ploegman, J.H. / Drent, G. / Kalk, K.H. / Hol, W.G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 67.2 KB | Display | ![]() |
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PDB format | ![]() | 52.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 426.8 KB | Display | ![]() |
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Full document | ![]() | 435 KB | Display | |
Data in XML | ![]() | 13.9 KB | Display | |
Data in CIF | ![]() | 18 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1boiC ![]() 1rhsS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 33240.668 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: SULFUR SUBSTITUTED AT RESIDUE CYS 247 / Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 37 % | ||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.3 / Details: pH 7.3 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 297 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: Oct 1, 1997 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→55 Å / Num. obs: 10362 / % possible obs: 78.8 % / Observed criterion σ(I): 0 / Redundancy: 5.9 % / Rmerge(I) obs: 0.11 / Rsym value: 0.11 |
Reflection shell | Resolution: 2.3→2.42 Å / Redundancy: 1.3 % / Rmerge(I) obs: 0.34 / Rsym value: 0.24 / % possible all: 13.4 |
Reflection | *PLUS Num. measured all: 60362 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1RHS Resolution: 2.3→55 Å / Data cutoff high absF: 1000000 / Data cutoff low absF: 0.0001 / Cross valid method: FREE R-VALUE / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.3→55 Å
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Refine LS restraints |
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Xplor file | Serial no: 1 / Param file: PARAM19X.PRO / Topol file: TOPH19X.PRO | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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