+Open data
-Basic information
Entry | Database: PDB / ID: 1bl8 | ||||||
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Title | POTASSIUM CHANNEL (KCSA) FROM STREPTOMYCES LIVIDANS | ||||||
Components | PROTEIN (POTASSIUM CHANNEL PROTEIN) | ||||||
Keywords | MEMBRANE PROTEIN / POTASSIUM CHANNEL / INTEGRAL MEMBRANE PROTEIN | ||||||
Function / homology | Function and homology information delayed rectifier potassium channel activity / voltage-gated potassium channel complex / identical protein binding Similarity search - Function | ||||||
Biological species | Streptomyces lividans (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIR / Resolution: 3.2 Å | ||||||
Authors | Doyle, D.A. / Cabral, J.M. / Pfuetzner, R.A. / Kuo, A. / Gulbis, J.M. / Cohen, S.L. / Chait, B.T. / Mackinnon, R. | ||||||
Citation | Journal: Science / Year: 1998 Title: The structure of the potassium channel: molecular basis of K+ conduction and selectivity. Authors: Doyle, D.A. / Morais Cabral, J. / Pfuetzner, R.A. / Kuo, A. / Gulbis, J.M. / Cohen, S.L. / Chait, B.T. / MacKinnon, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1bl8.cif.gz | 74.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1bl8.ent.gz | 58.3 KB | Display | PDB format |
PDBx/mmJSON format | 1bl8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1bl8_validation.pdf.gz | 449.6 KB | Display | wwPDB validaton report |
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Full document | 1bl8_full_validation.pdf.gz | 479.6 KB | Display | |
Data in XML | 1bl8_validation.xml.gz | 19.3 KB | Display | |
Data in CIF | 1bl8_validation.cif.gz | 24.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bl/1bl8 ftp://data.pdbj.org/pub/pdb/validation_reports/bl/1bl8 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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-Components
#1: Protein | Mass: 10252.959 Da / Num. of mol.: 4 / Mutation: L90C Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces lividans (bacteria) / Plasmid: PQE60 / Gene (production host): KCSA / Production host: Escherichia coli (E. coli) / Strain (production host): XL-1 BLUE / References: UniProt: P0A334 #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 6 |
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-Sample preparation
Crystal | Density Matthews: 4.99 Å3/Da / Density % sol: 75.34 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 Details: ONE-TO-MIXTURE OF PROTEIN SOLUTION AND RESERVOIR (200 MM CACL2, 100 MM HEPES PH 7.5, 48% PEG 400). | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / Method: vapor diffusion, sitting dropDetails: drop consists of equal volume of protein and reservoir solutions | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: CHESS / Beamline: A1 / Wavelength: 0.908 |
Detector | Type: PRINCETON 2K / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.908 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→30 Å / Num. obs: 12603 / % possible obs: 93.3 % / Observed criterion σ(I): 2 / Redundancy: 6.1 % / Biso Wilson estimate: 90 Å2 / Rmerge(I) obs: 0.086 |
Reflection shell | Resolution: 3.2→3.3 Å / Redundancy: 2.3 % / % possible all: 66.6 |
Reflection shell | *PLUS % possible obs: 66.6 % / Rmerge(I) obs: 0.286 / Mean I/σ(I) obs: 3.9 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 3.2→10 Å / Isotropic thermal model: GROUP / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 3.2→10 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: RESTRAINTS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: X-PLOR / Version: 3.851 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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