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- PDB-1bl5: ISOCITRATE DEHYDROGENASE FROM E. COLI SINGLE TURNOVER LAUE STRUCT... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1bl5 | ||||||
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Title | ISOCITRATE DEHYDROGENASE FROM E. COLI SINGLE TURNOVER LAUE STRUCTURE OF RATE-LIMITED PRODUCT COMPLEX, 10 MSEC TIME RESOLUTION | ||||||
![]() | ISOCITRATE DEHYDROGENASE | ||||||
![]() | OXIDOREDUCTASE / NAD(A)-CHOH(D) / PHOSPHORYLATION | ||||||
Function / homology | ![]() isocitrate dehydrogenase (NADP+) / isocitrate dehydrogenase (NADP+) activity / glyoxylate cycle / guanosine tetraphosphate binding / tricarboxylic acid cycle / electron transport chain / NAD binding / response to oxidative stress / magnesium ion binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Stoddard, B.L. / Cohen, B. / Brubaker, M. / Mesecar, A. / Koshland Junior, D.E. | ||||||
![]() | ![]() Title: Millisecond Laue structures of an enzyme-product complex using photocaged substrate analogs. Authors: Stoddard, B.L. / Cohen, B.E. / Brubaker, M. / Mesecar, A.D. / Koshland Jr., D.E. #1: ![]() Title: Catalytic Mechanism of Nadp(+)-Dependent Isocitrate Dehydrogenase: Implications from the Structures of Magnesium-Isocitrate and Nadp+ Complexes Authors: Hurley, J.H. / Dean, A.M. / Koshland Junior, D.E. / Stroud, R.M. #2: ![]() Title: Regulation of Isocitrate Dehydrogenase by Phosphorylation Involves No Long-Range Conformational Change in the Free Enzyme Authors: Hurley, J.H. / Dean, A.M. / Thorsness, P.E. / Koshland Junior, D.E. / Stroud, R.M. #3: ![]() Title: Regulation of an Enzyme by Phosphorylation at the Active Site Authors: Hurley, J.H. / Dean, A.M. / Sohl, J.L. / Koshland Junior, D.E. / Stroud, R.M. #4: ![]() Title: Structure of a Bacterial Enzyme Regulated by Phosphorylation, Isocitrate Dehydrogenase Authors: Hurley, J.H. / Thorsness, P.E. / Ramalingam, V. / Helmers, N.H. / Koshland Junior, D.E. / Stroud, R.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 96.3 KB | Display | ![]() |
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PDB format | ![]() | 72 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 464.1 KB | Display | ![]() |
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Full document | ![]() | 477.9 KB | Display | |
Data in XML | ![]() | 11.6 KB | Display | |
Data in CIF | ![]() | 17.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 45549.258 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: RATE-LIMITED ENOLATE INTERMEDIATE / Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P08200, isocitrate dehydrogenase (NADP+) |
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#2: Chemical | ChemComp-MG / |
#3: Chemical | ChemComp-AKG / |
#4: Chemical | ChemComp-NAP / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.7 Å3/Da / Density % sol: 74 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 / Details: pH 7.5 | ||||||||||||||||||||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 5.4 / Method: unknownDetails: Hurley, J.H., (1989) Proc.Nat.Acad.Sci.USA, 86, 8635. | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 274 K | |||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||
Detector | Type: FUJI / Detector: IMAGE PLATE / Date: Jan 1, 1995 | |||||||||
Radiation | Protocol: LAUE / Monochromatic (M) / Laue (L): L / Scattering type: neutron | |||||||||
Radiation wavelength |
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Reflection | Resolution: 2.5→50 Å / Num. obs: 21751 / % possible obs: 90.8 % / Redundancy: 12.9 % / Rmerge(I) obs: 0.102 / Net I/σ(I): 17.2 | |||||||||
Reflection shell | Resolution: 2.4→2.5 Å / Redundancy: 16.4 % / Rmerge(I) obs: 0.237 / Mean I/σ(I) obs: 7.5 / % possible all: 83.8 | |||||||||
Reflection | *PLUS Num. measured all: 360784 |
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Processing
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Refinement | Method to determine structure: OTHER / Resolution: 2.5→50 Å / Rfactor Rfree error: 0.02 / Cross valid method: THROUGHOUT / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.5→50 Å
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Refine LS restraints |
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