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- PDB-1bjz: TETRACYCLINE CHELATED MG2+-ION INITIATES HELIX UNWINDING FOR TET ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1bjz | ||||||
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Title | TETRACYCLINE CHELATED MG2+-ION INITIATES HELIX UNWINDING FOR TET REPRESSOR INDUCTION | ||||||
![]() | TETRACYCLINE REPRESSOR | ||||||
![]() | TRANSCRIPTION REGULATION / DNA-BINDING PROTEIN / HTH-MOTIF | ||||||
Function / homology | ![]() transcription cis-regulatory region binding / DNA-binding transcription factor activity / response to antibiotic / negative regulation of DNA-templated transcription / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Orth, P. / Saenger, W. / Hinrichs, W. | ||||||
![]() | ![]() Title: Tetracycline-chelated Mg2+ ion initiates helix unwinding in Tet repressor induction. Authors: Orth, P. / Saenger, W. / Hinrichs, W. #1: ![]() Title: Conformational Changes of the Tet Repressor Induced by Tetracycline Trapping Authors: Orth, P. / Cordes, F. / Schnappinger, D. / Hillen, W. / Saenger, W. / Hinrichs, W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 49.3 KB | Display | ![]() |
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PDB format | ![]() | 36 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 422.3 KB | Display | ![]() |
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Full document | ![]() | 426.2 KB | Display | |
Data in XML | ![]() | 9.5 KB | Display | |
Data in CIF | ![]() | 11.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2tctS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 23288.334 Da / Num. of mol.: 1 / Mutation: A2S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 40 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 8 / Details: pH 8.0 | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 287 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Aug 1, 1996 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→23.6 Å / Num. obs: 32245 / % possible obs: 89 % / Redundancy: 3.1 % / Biso Wilson estimate: 47.3 Å2 / Rsym value: 0.083 / Net I/σ(I): 8.2 |
Reflection shell | Resolution: 2.2→2.28 Å / Mean I/σ(I) obs: 4.1 / Rsym value: 0.36 / % possible all: 93.1 |
Reflection | *PLUS Num. obs: 10487 / % possible obs: 89.5 % / Rmerge(I) obs: 0.083 |
Reflection shell | *PLUS Lowest resolution: 2.3 Å / Rmerge(I) obs: 0.363 / Mean I/σ(I) obs: 3.8 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2TCT Resolution: 2.2→23.6 Å / Data cutoff high absF: 10000000 / Data cutoff low absF: 0.001 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT
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Displacement parameters | Biso mean: 57.6 Å2
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Refinement step | Cycle: LAST / Resolution: 2.2→23.6 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.28 Å / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.383 |