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- PDB-1bhu: THE 3D STRUCTURE OF THE STREPTOMYCES METALLOPROTEINASE INHIBITOR,... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1bhu | ||||||
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Title | THE 3D STRUCTURE OF THE STREPTOMYCES METALLOPROTEINASE INHIBITOR, SMPI, ISOLATED FROM STREPTOMYCES NIGRESCENS TK-23, NMR, MINIMIZED AVERAGE STRUCTURE | ||||||
![]() | METALLOPROTEINASE INHIBITOR | ||||||
![]() | METALLOPROTEINASE INHIBITOR | ||||||
Function / homology | Antimicrobial/protein inhibitor, gamma-crystallin-like / Gamma-B Crystallin; domain 1 - #30 / Gamma-B Crystallin; domain 1 / metalloendopeptidase inhibitor activity / Gamma-crystallin-like / Sandwich / Mainly Beta / Metalloproteinase inhibitor![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Tate, S. / Ohno, A. / Seeram, S.S. / Hiraga, K. / Oda, K. / Kainosho, M. | ||||||
![]() | ![]() Title: NMR structure of the Streptomyces metalloproteinase inhibitor, SMPI, isolated from Streptomyces nigrescens TK-23: another example of an ancestral beta gamma-crystallin precursor structure. Authors: Ohno, A. / Tate, S. / Seeram, S.S. / Hiraga, K. / Swindells, M.B. / Oda, K. / Kainosho, M. #1: ![]() Title: Elucidation of the Mode of Interaction of Thermolysin with a Proteinaceous Metalloproteinase Inhibitor, Smpi, Based on a Model Complex Structure and a Structural Dynamics Analysis Authors: Tate, S. / Ohno, A. / Seeram, S.S. / Hiraga, K. / Oda, K. / Kainosho, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 39.1 KB | Display | ![]() |
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PDB format | ![]() | 30.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 243.8 KB | Display | ![]() |
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Full document | ![]() | 243.6 KB | Display | |
Data in XML | ![]() | 5.4 KB | Display | |
Data in CIF | ![]() | 6.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 10374.746 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: HETERONUCLEAR MULTI-DIMENSIONAL EXPERIMENT |
NMR details | Text: THE STRUCTURE WAS DETEMINED ON THE BASIS OF THE HETERO-NUCLEAR MUTLIDIMENSIONAL NMR TECHNIQUES USING 13C, 15N ENRICHED PROTEINS. |
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Sample preparation
Details | Contents: WATER |
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Sample conditions | Ionic strength: NO SALT / pH: 3.3 / Pressure: 1 atm / Temperature: 310 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DMX500 / Manufacturer: Bruker / Model: DMX500 / Field strength: 500 MHz |
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Processing
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NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | ||||||||||||
NMR ensemble | Conformer selection criteria: LEAST VIOLATION / Conformers calculated total number: 60 / Conformers submitted total number: 1 |