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- PDB-1bh6: SUBTILISIN DY IN COMPLEX WITH THE SYNTHETIC INHIBITOR N-BENZYLOXY... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1bh6 | ||||||
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Title | SUBTILISIN DY IN COMPLEX WITH THE SYNTHETIC INHIBITOR N-BENZYLOXYCARBONYL-ALA-PRO-PHE-CHLOROMETHYL KETONE | ||||||
![]() | SUBTILISIN DY | ||||||
![]() | HYDROLASE / SUBTILISIN / PROTEIN DEGRADATION | ||||||
Function / homology | ![]() subtilisin / sporulation resulting in formation of a cellular spore / serine-type endopeptidase activity / extracellular region / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Eschenburg, S. / Genov, N. / Wilson, K.S. / Betzel, C. | ||||||
![]() | ![]() Title: Crystal structure of subtilisin DY, a random mutant of subtilisin Carlsberg. Authors: Eschenburg, S. / Genov, N. / Peters, K. / Fittkau, S. / Stoeva, S. / Wilson, K.S. / Betzel, C. #1: ![]() Title: Crystallization and Preliminary X-Ray Analysis of Subtilisin Dy, a Natural Mutant of Subtilisin Carlsberg Authors: Betzel, C. / Visanji, M. / Eschenburg, S. / Wilson, K.S. / Peters, K. / Fittkau, S. / Singh, T.P. / Genov, N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 64.6 KB | Display | ![]() |
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PDB format | ![]() | 49.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1cseS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 27499.416 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: X-IRRADIATED JAPANESE STRAIN OF BACILLUS LICHENIFORMIS Source: (natural) ![]() ![]() |
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#2: Chemical | ChemComp-CA / |
#3: Chemical | ChemComp-NA / |
#4: Chemical | ChemComp-1BH / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.1 Å3/Da / Density % sol: 41 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 6 / Details: AS GIVEN IN REFERENCE 1, pH 6.0 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 16 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 278 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Feb 1, 1993 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→10 Å / Num. obs: 22887 / % possible obs: 96.7 % / Observed criterion σ(I): 0 / Redundancy: 4.4 % / Rmerge(I) obs: 0.03 / Rsym value: 0.03 / Net I/σ(I): 23 |
Reflection shell | Resolution: 1.75→1.79 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.052 / Mean I/σ(I) obs: 14 / Rsym value: 0.052 / % possible all: 92.2 |
Reflection | *PLUS Num. measured all: 101587 |
Reflection shell | *PLUS % possible obs: 92.2 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1CSE Resolution: 1.75→10 Å / σ(F): 0 /
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Refine analyze | Luzzati sigma a obs: 0.12 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.75→10 Å
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Refine LS restraints |
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