+Open data
-Basic information
Entry | Database: PDB / ID: 1bg2 | ||||||
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Title | HUMAN UBIQUITOUS KINESIN MOTOR DOMAIN | ||||||
Components | KINESIN | ||||||
Keywords | MOTOR PROTEIN / ATPASE / MICROTUBULE ASSOCIATED | ||||||
Function / homology | Function and homology information cytoplasm organization / cytolytic granule membrane / plus-end-directed vesicle transport along microtubule / anterograde neuronal dense core vesicle transport / anterograde dendritic transport of neurotransmitter receptor complex / mitocytosis / retrograde neuronal dense core vesicle transport / anterograde axonal protein transport / vesicle transport along microtubule / lysosome localization ...cytoplasm organization / cytolytic granule membrane / plus-end-directed vesicle transport along microtubule / anterograde neuronal dense core vesicle transport / anterograde dendritic transport of neurotransmitter receptor complex / mitocytosis / retrograde neuronal dense core vesicle transport / anterograde axonal protein transport / vesicle transport along microtubule / lysosome localization / positive regulation of potassium ion transport / natural killer cell mediated cytotoxicity / Kinesins / plus-end-directed microtubule motor activity / RHO GTPases activate KTN1 / stress granule disassembly / COPI-dependent Golgi-to-ER retrograde traffic / mitochondrion transport along microtubule / ciliary rootlet / centrosome localization / kinesin complex / synaptic vesicle transport / microtubule motor activity / microtubule-based movement / Insulin processing / centriolar satellite / Signaling by ALK fusions and activated point mutants / Nuclear events stimulated by ALK signaling in cancer / phagocytic vesicle / axon cytoplasm / MHC class II antigen presentation / dendrite cytoplasm / regulation of membrane potential / axon guidance / positive regulation of synaptic transmission, GABAergic / positive regulation of protein localization to plasma membrane / cellular response to type II interferon / microtubule binding / vesicle / microtubule / cadherin binding / protein-containing complex binding / perinuclear region of cytoplasm / ATP hydrolysis activity / mitochondrion / ATP binding / membrane / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIR / Resolution: 1.8 Å | ||||||
Authors | Kull, F.J. / Sablin, E.P. / Lau, R. / Fletterick, R.J. / Vale, R.D. | ||||||
Citation | Journal: Nature / Year: 1996 Title: Crystal structure of the kinesin motor domain reveals a structural similarity to myosin. Authors: Kull, F.J. / Sablin, E.P. / Lau, R. / Fletterick, R.J. / Vale, R.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1bg2.cif.gz | 80.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1bg2.ent.gz | 60.1 KB | Display | PDB format |
PDBx/mmJSON format | 1bg2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bg/1bg2 ftp://data.pdbj.org/pub/pdb/validation_reports/bg/1bg2 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 36405.070 Da / Num. of mol.: 1 / Fragment: MOTOR DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Cell line: BL21 / Organ: UBIQUITOUS / Plasmid: PET / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P33176 | ||||
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#2: Chemical | ChemComp-MG / | ||||
#3: Chemical | #4: Chemical | ChemComp-ADP / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.54 Å3/Da / Density % sol: 51.9 % |
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Crystal grow | pH: 4.6 Details: 5 MG/ML PROTEIN, 50 MM NA ACETATE PH 4.6, 75 MM KCL, 3.5% PEG 4K, 2.5 MM ATP, 10 MM MGCL2 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL7-1 / Wavelength: 1.08 |
Detector | Type: MAR scanner 180 mm plate / Detector: IMAGE PLATE / Date: Jun 1, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Highest resolution: 1.8 Å / Num. obs: 30582 / % possible obs: 82.4 % / Observed criterion σ(I): 2 / Rsym value: 0.061 / Net I/σ(I): 12.5 |
Reflection shell | Resolution: 1.77→1.84 Å / Mean I/σ(I) obs: 2.1 / Rsym value: 0.303 / % possible all: 49.2 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 1.8→6 Å / Data cutoff high absF: 10000000 / Data cutoff low absF: 0.001 / Cross valid method: THROUGHOUT / σ(F): 2
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Displacement parameters | Biso mean: 31.5 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→6 Å
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LS refinement shell | Resolution: 1.8→1.88 Å / Total num. of bins used: 8
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