+Open data
-Basic information
Entry | Database: PDB / ID: 1b7e | ||||||
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Title | TRANSPOSASE INHIBITOR | ||||||
Components | PROTEIN (TRANSPOSASE INHIBITOR PROTEIN FROM TN5) | ||||||
Keywords | TRANSFERASE INHIBITOR / TRANSPOSASE / POLYNUCLEOTIDYL TRANSFERASE / HYDROLASE | ||||||
Function / homology | Function and homology information transposase activity / DNA transposition / endonuclease activity / Hydrolases; Acting on ester bonds / DNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.9 Å | ||||||
Authors | Davies, D.R. / Braam, L.M. / Reznikoff, W.S. / Rayment, I. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 1999 Title: The three-dimensional structure of a Tn5 transposase-related protein determined to 2.9A resolution. Authors: Davies, D.R. / Braam, L.M. / Reznikoff, W.S. / Rayment, I. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1b7e.cif.gz | 88.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1b7e.ent.gz | 65.4 KB | Display | PDB format |
PDBx/mmJSON format | 1b7e.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1b7e_validation.pdf.gz | 515.1 KB | Display | wwPDB validaton report |
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Full document | 1b7e_full_validation.pdf.gz | 568.5 KB | Display | |
Data in XML | 1b7e_validation.xml.gz | 16.4 KB | Display | |
Data in CIF | 1b7e_validation.cif.gz | 23.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b7/1b7e ftp://data.pdbj.org/pub/pdb/validation_reports/b7/1b7e | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 47575.547 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / References: GenBank: 622948, UniProt: Q46731*PLUS | ||||
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#2: Chemical | #3: Water | ChemComp-HOH / | Sequence details | THE FOLLOWING RESIDUES WERE DISORDERED BEYOND THE BETA-CARBON AND THUS WERE MODELED AS ALANINE: ...THE FOLLOWING RESIDUES WERE DISORDERED | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.84 Å3/Da / Density % sol: 57 % / Description: STRUCTURE WAS INDEPENDENTLY SOLVED USING MIR | |||||||||||||||||||||||||
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Crystal grow | pH: 6 / Details: pH 6.0 | |||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 57 % | |||||||||||||||||||||||||
Crystal grow | *PLUS Method: batch method | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 113 K | ||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 1.0273,1.0720,1.1201 | ||||||||||||
Detector | Type: BRUKER / Detector: CCD / Details: MIRROR | ||||||||||||
Radiation | Monochromator: DOUBLE CRYSTAL MONOCHROMATOR / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.9→30 Å / Num. all: 13121 / Num. obs: 13121 / % possible obs: 94.3 % / Redundancy: 2.98 % / Rsym value: 0.05 / Net I/σ(I): 30.9 | ||||||||||||
Reflection shell | Resolution: 2.9→3 Å / Mean I/σ(I) obs: 7.5 / Rsym value: 0.204 / % possible all: 87 | ||||||||||||
Reflection | *PLUS Num. measured all: 69105 / Rmerge(I) obs: 0.05 | ||||||||||||
Reflection shell | *PLUS % possible obs: 87 % / Rmerge(I) obs: 0.204 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 2.9→30 Å / σ(F): 0 / Stereochemistry target values: TNT PROTGEO
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Refinement step | Cycle: LAST / Resolution: 2.9→30 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.9 Å / Lowest resolution: 30 Å / σ(F): 0 / Rfactor obs: 0.195 | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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