+Open data
-Basic information
Entry | Database: PDB / ID: 1b4o | ||||||
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Title | NMR STUDY OF SSO7D MUTANT (F31A) MINIMIZED AVERAGE STRUCTURE | ||||||
Components | ENDORIBONUCLEASE P2 | ||||||
Keywords | DNA BINDING PROTEIN / RNASE AND DNA-BINDING PROTEIN / THERMOSTABLE RIBONUCLEASE / SULFOLOBUS SOLFATARICUS / DNA-BINDING PROTEIN | ||||||
Function / homology | Function and homology information Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters / RNA endonuclease activity / DNA binding Similarity search - Function | ||||||
Biological species | Sulfolobus solfataricus (archaea) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Consonni, R. / Santomo, L. / Zetta, L. | ||||||
Citation | Journal: Biochemistry / Year: 1999 Title: A single-point mutation in the extreme heat- and pressure-resistant sso7d protein from sulfolobus solfataricus leads to a major rearrangement of the hydrophobic core. Authors: Consonni, R. / Santomo, L. / Fusi, P. / Tortora, P. / Zetta, L. #1: Journal: Biochemistry / Year: 1998 Title: Differential Scanning Calorimetry Study of the Thermodynamic Stability of Some Mutants of Sso7D from Sulfolobus Solfataricus Authors: Catanzano, F. / Graziano, G. / Fusi, P. / Tortora, P. / Barone, G. #2: Journal: Proteins / Year: 1997 Title: Extreme Heat-and Pressure-Resistant 7-kDa Protein P2 from the Archaeon Sulfolobus Solfataricus is Dramatically Destabilized by a Single-Point Amino Acid Substitution Authors: Fusi, P. / Goossens, K. / Consonni, R. / Grisa, M. / Puricelli, P. / Vecchio, G. / Vanoni, M. / Zetta, L. / Heremans, K. / Tortora, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1b4o.cif.gz | 26.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1b4o.ent.gz | 20.4 KB | Display | PDB format |
PDBx/mmJSON format | 1b4o.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b4/1b4o ftp://data.pdbj.org/pub/pdb/validation_reports/b4/1b4o | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6958.127 Da / Num. of mol.: 1 / Mutation: F31A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sulfolobus solfataricus (archaea) / Plasmid: PT7-7 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) PLYSE References: UniProt: P61991, Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: THE MINIMIZED AVERAGE STRUCTURE WAS DETERMINED USING 22 SELECTED CONFORMERS |
-Sample preparation
Details | Contents: H2O |
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Sample conditions | pH: 4.5 / Temperature: 300 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST ENERGY TERM / Conformers calculated total number: 50 / Conformers submitted total number: 1 |