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- PDB-2orc: CRO REPRESSOR INSERTION MUTANT K56-[DGEVK], NMR, 32 STRUCTURES -

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Basic information

Entry
Database: PDB / ID: 2orc
TitleCRO REPRESSOR INSERTION MUTANT K56-[DGEVK], NMR, 32 STRUCTURES
ComponentsCRO REPRESSOR
KeywordsGENE REGULATING PROTEIN
Function / homology
Function and homology information


latency-replication decision / release from viral latency / negative regulation of transcription by competitive promoter binding / negative regulation of viral transcription / core promoter sequence-specific DNA binding / response to UV / protein homodimerization activity / DNA binding
Similarity search - Function
CRO Repressor / Regulatory protein cro superfamily / Cro / Regulatory protein cro / CRO Repressor / Lambda repressor-like, DNA-binding domain superfamily / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Regulatory protein cro
Similarity search - Component
Biological speciesEnterobacteria phage lambda (virus)
MethodSOLUTION NMR / DISTANCE GEOMETRY, SIMULATED ANNEALING
AuthorsMossing, M.C.
Citation
Journal: Protein Sci. / Year: 1998
Title: Solution structure and dynamics of a designed monomeric variant of the lambda Cro repressor.
Authors: Mossing, M.C.
#1: Journal: Science / Year: 1990
Title: Stable, Monomeric Variants of Lambda Cro Obtained by Insertion of a Designed Beta-Hairpin Sequence
Authors: Mossing, M.C. / Sauer, R.T.
History
DepositionJan 20, 1998Processing site: BNL
Revision 1.0May 27, 1998Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 16, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site / _struct_ref_seq_dif.details
Revision 1.4May 22, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: CRO REPRESSOR


Theoretical massNumber of molelcules
Total (without water)7,9051
Polymers7,9051
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)32 / 50NO DISTANCE VIOLATION > 0.5A, NO DIHEDRAL VIOLATION > 5 DEGREES
Representative

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Components

#1: Protein CRO REPRESSOR


Mass: 7905.048 Da / Num. of mol.: 1 / Mutation: INS(K56-DGEVK)
Source method: isolated from a genetically manipulated source
Details: RESULTS IN A 71-RESIDUE STABLE MONOMER MUTANT / Source: (gene. exp.) Enterobacteria phage lambda (virus) / Genus: Lambda-like viruses / Gene: CRO MUTANT K56-[DGEVK] / Plasmid: PUCRO.MDG / Gene (production host): CRO MUTANT K56-[DGEVK] / Production host: Escherichia coli (E. coli) / Strain (production host): X90 / References: UniProt: P03040

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
111NOESY
121COSY
131TOCSY
141HSQC-NOESY
151HSQC-TOCSY

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Sample preparation

Sample conditionspH: 4.6 / Temperature: 298 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Varian UNITYVarianUNITY5001
Varian UNITYPLUSVarianUNITYPLUS6002

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Processing

Software
NameClassification
X-PLORmodel building
X-PLORrefinement
X-PLORphasing
NMR software
NameDeveloperClassification
X-PLORBRUNGERrefinement
X-PLORstructure solution
RefinementMethod: DISTANCE GEOMETRY, SIMULATED ANNEALING / Software ordinal: 1
Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL REFERENCE.
NMR ensembleConformer selection criteria: NO DISTANCE VIOLATION > 0.5A, NO DIHEDRAL VIOLATION > 5 DEGREES
Conformers calculated total number: 50 / Conformers submitted total number: 32

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