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Yorodumi- PDB-2kh9: Solution structure of yeast Prp24-RRM2 bound to a fragment of U6 RNA -
+Open data
-Basic information
Entry | Database: PDB / ID: 2kh9 | ||||||
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Title | Solution structure of yeast Prp24-RRM2 bound to a fragment of U6 RNA | ||||||
Components |
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Keywords | SPLICING/RNA / Protein/RNA / RRM / snRNP / Splicing / mRNA processing / mRNA splicing / Nucleus / Phosphoprotein / RNA-binding / SPLICING-RNA COMPLEX | ||||||
Function / homology | Function and homology information U6 snRNP / snRNA binding / spliceosomal complex assembly / spliceosomal tri-snRNP complex assembly / U6 snRNA binding / spliceosomal complex / mRNA splicing, via spliceosome / ribonucleoprotein complex / RNA binding / nucleus Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | SOLUTION NMR / simulated annealing, torsion angle dynamics | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Martin-Tumasz, S.A. / Butcher, S.E. | ||||||
Citation | Journal: Rna / Year: 2010 Title: Structure and functional implications of a complex containing a segment of U6 RNA bound by a domain of Prp24. Authors: Martin-Tumasz, S. / Reiter, N.J. / Brow, D.A. / Butcher, S.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kh9.cif.gz | 324.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kh9.ent.gz | 264.8 KB | Display | PDB format |
PDBx/mmJSON format | 2kh9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2kh9_validation.pdf.gz | 383.5 KB | Display | wwPDB validaton report |
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Full document | 2kh9_full_validation.pdf.gz | 521.1 KB | Display | |
Data in XML | 2kh9_validation.xml.gz | 22.2 KB | Display | |
Data in CIF | 2kh9_validation.cif.gz | 35.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kh/2kh9 ftp://data.pdbj.org/pub/pdb/validation_reports/kh/2kh9 | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10841.389 Da / Num. of mol.: 1 / Fragment: UNP residues 115-197 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: PRP24, YMR268C, YM8156.10C / Plasmid: pET21b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: P49960 |
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#2: RNA chain | Mass: 1939.237 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: Synthetic RNA |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 50 / pH: 7 / Pressure: ambient / Temperature: 298.15 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 1466 / NOE intraresidue total count: 230 / NOE long range total count: 427 / NOE medium range total count: 353 / NOE sequential total count: 456 / Hydrogen bond constraints total count: 43 / Protein chi angle constraints total count: 0 / Protein other angle constraints total count: 0 / Protein phi angle constraints total count: 43 / Protein psi angle constraints total count: 43 | ||||||||||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 / Maximum lower distance constraint violation: 0 Å / Maximum torsion angle constraint violation: 7.5 ° / Maximum upper distance constraint violation: 0.38 Å / Torsion angle constraint violation method: HADDOCK Output | ||||||||||||||||||||||||||||||||||||||||||||||||
NMR ensemble rms | Distance rms dev: 0.015 Å / Distance rms dev error: 0.002 Å |