|Entry||Database: PDB / ID: 1b2g|
|Title||PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 9.00 COORDINATES)|
|Keywords||HORMONE/GROWTH FACTOR / HORMONE / HORMONE-GROWTH FACTOR COMPLEX|
|Function / homology|
Function and homology information
glycoprotein biosynthetic process / lactate biosynthetic process / positive regulation of lipoprotein lipase activity / positive regulation of fatty acid biosynthetic process / response to L-arginine / lipoprotein biosynthetic process / positive regulation of glucose metabolic process / lipid biosynthetic process / alpha-beta T cell activation / negative regulation of glycogen catabolic process ...glycoprotein biosynthetic process / lactate biosynthetic process / positive regulation of lipoprotein lipase activity / positive regulation of fatty acid biosynthetic process / response to L-arginine / lipoprotein biosynthetic process / positive regulation of glucose metabolic process / lipid biosynthetic process / alpha-beta T cell activation / negative regulation of glycogen catabolic process / negative regulation of NAD(P)H oxidase activity / nitric oxide-cGMP-mediated signaling pathway / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / positive regulation of respiratory burst / negative regulation of respiratory burst involved in inflammatory response / negative regulation of gluconeogenesis / regulation of cellular amino acid metabolic process / negative regulation of acute inflammatory response / negative regulation of reactive oxygen species biosynthetic process / regulation of protein localization to plasma membrane / positive regulation of dendritic spine maintenance / positive regulation of glycogen biosynthetic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of protein secretion / negative regulation of lipid catabolic process / fatty acid homeostasis / positive regulation of insulin receptor signaling pathway / neuron projection maintenance / insulin-like growth factor receptor binding / positive regulation of DNA replication / positive regulation of protein autophosphorylation / positive regulation of glycolytic process / regulation of transmembrane transporter activity / positive regulation of mitotic nuclear division / positive regulation of cytokine production / acute-phase response / activation of protein kinase B activity / positive regulation of glucose import / hormone activity / negative regulation of proteolysis / negative regulation of protein catabolic process / insulin receptor binding / positive regulation of protein localization to nucleus / insulin receptor signaling pathway / vasodilation / glucose metabolic process / glucose homeostasis / wound healing / positive regulation of peptidyl-tyrosine phosphorylation / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase signaling / protease binding / positive regulation of NF-kappaB transcription factor activity / positive regulation of protein kinase B signaling / positive regulation of cell migration / G protein-coupled receptor signaling pathway / positive regulation of cell population proliferation / extracellular space / identical protein binding
Similarity search - Function
Insulin / Insulin family / Insulin-like / Insulin/IGF/Relaxin family / Insulin / insulin-like growth factor / relaxin family. / Insulin, conserved site / Insulin-like superfamily / Insulin family signature.
Similarity search - Domain/homology
|Biological species||Sus scrofa (pig)|
|Method||X-RAY DIFFRACTION / OTHER / Resolution: 1.8 Å|
|Authors||Diao, J.S. / Caspar, D.L.D.|
Journal: Acta Crystallogr.,Sect.D / Year: 2003
Title: Crystallographic titration of cubic insulin crystals: pH affects GluB13 switching and sulfate binding.
Authors: Diao, J.
#1: Journal: Biophys.J. / Year: 1992
Title: Conformational Changes in Cubic Insulin Crystals in the Ph Range 7-11
Authors: Gursky, O. / Badger, J. / Li, Y. / Caspar, D.L.
#2: Journal: Biophys.J. / Year: 1992
Title: Monovalent Cation Binding in Cubic Insulin Crystals
Authors: Gursky, O. / Li, Y. / Badger, J. / Caspar, D.L.
#4: Journal: Acta Crystallogr.,Sect.B / Year: 1991
Title: Structure of the Pig Insulin Dimer in the Cubic Crystal
Authors: Badger, J. / Harris, M.R. / Reynolds, C.D. / Evans, A.C. / Dodson, E.J. / Dodson, G.G. / North, A.C.T.
#5: Journal: Proc.Natl.Acad.Sci.USA / Year: 1991
Title: Water Structure in Cubic Insulin Crystals
Authors: Badger, J. / Caspar, D.L.
|Structure viewer||Molecule: |
Downloads & links
A: PROTEIN (INSULIN a chain)
B: PROTEIN (INSULIN b chain)
|Components on special symmetry positions|
|#1: Protein/peptide|| |
Mass: 2383.698 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / Organ: PANCREAS / References: UniProt: P01315
|#2: Protein/peptide|| |
Mass: 3403.927 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / Organ: PANCREAS / References: UniProt: P01315
|#3: Water|| ChemComp-HOH / |
|Experiment||Method: X-RAY DIFFRACTION / Number of used crystals: 1|
|Crystal||Density Matthews: 3.53 Å3/Da / Density % sol: 65 %|
|Crystal grow||pH: 9 / Details: pH 9.00|
|Components of the solutions||Name: 1M SODIUM SULFATE SOLUTION AT PH 9.00|
*PLUSpH: 9.2 / Method: microdialysis
|Components of the solutions|
|Diffraction||Mean temperature: 287 K|
|Diffraction source||Wavelength: 1.5418|
|Radiation||Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray|
|Radiation wavelength||Wavelength: 1.5418 Å / Relative weight: 1|
|Reflection||Resolution: 1.7→55 Å / Num. obs: 8672 / % possible obs: 94.8 % / Redundancy: 4 % / Biso Wilson estimate: 21.33 Å2 / Rsym value: 0.065|
*PLUSHighest resolution: 1.8 Å / % possible obs: 94.85 % / Rmerge(I) obs: 0.065
|Refinement||Method to determine structure: OTHER / Resolution: 1.8→10 Å / Data cutoff high absF: 1000000 / Data cutoff low absF: 0.001 / Cross valid method: THROUGHOUT / σ(F): 2 |
|Displacement parameters||Biso mean: 23.81 Å2|
|Refinement step||Cycle: LAST / Resolution: 1.8→10 Å|
|Refine LS restraints|
|LS refinement shell||Resolution: 1.8→1.84 Å / Total num. of bins used: 15 |
*PLUSHighest resolution: 1.8 Å / Lowest resolution: 10 Å / Rfactor Rfree: 0.224 / Rfactor Rwork: 0.2023
|Refine LS restraints|
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