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- PDB-1aqk: THREE-DIMENSIONAL STRUCTURE OF A HUMAN FAB WITH HIGH AFFINITY FOR... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1aqk | |||||||||
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Title | THREE-DIMENSIONAL STRUCTURE OF A HUMAN FAB WITH HIGH AFFINITY FOR TETANUS TOXOID | |||||||||
![]() | (FAB B7-15A2) x 2 | |||||||||
![]() | ![]() ![]() | |||||||||
Function / homology | ![]() IgD immunoglobulin complex / IgM immunoglobulin complex / IgA immunoglobulin complex / IgE immunoglobulin complex / CD22 mediated BCR regulation / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Faber, C. / Fan, Z. / Edmundson, A.B. | |||||||||
![]() | ![]() Title: Three-dimensional structure of a human Fab with high affinity for tetanus toxoid. Authors: Faber, C. / Shan, L. / Fan, Z. / Guddat, L.W. / Furebring, C. / Ohlin, M. / Borrebaeck, C.A. / Edmundson, A.B. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 98.6 KB | Display | ![]() |
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PDB format | ![]() | 77.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2fb4S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 22699.068 Da / Num. of mol.: 1 / Fragment: FAB / Source method: isolated from a natural source / Details: IMMUNOGLOBULIN HUMAN FAB / Source: (natural) ![]() ![]() |
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#2: Antibody | Mass: 24290.426 Da / Num. of mol.: 1 / Fragment: FAB / Source method: isolated from a natural source / Details: IMMUNOGLOBULIN HUMAN FAB / Source: (natural) ![]() ![]() |
#3: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.46 Å3/Da / Density % sol: 49.98 % | ||||||||||||||||||||||||||||||
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Crystal grow![]() | pH: 8.5 / Details: pH 8.5 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: unknown / Details: used to seeding | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 286 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: May 1, 1992 / Details: MIRRORS |
Radiation | Monochromator: NO / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.84→20 Å / Num. obs: 32194 / % possible obs: 81 % / Observed criterion σ(I): 2 / Redundancy: 2.5 % / Biso Wilson estimate: 20 Å2 / Rmerge(I) obs: 0.046 / Net I/σ(I): 33.1 |
Reflection shell | Resolution: 1.84→1.96 Å / Redundancy: 1.4 % / Rmerge(I) obs: 0.14 / Mean I/σ(I) obs: 8.5 / % possible all: 49 |
Reflection | *PLUS Num. all: 39263 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 2FB4 Resolution: 1.84→20 Å / Isotropic thermal model: TNT BCORREL / σ(F): 0 / Stereochemistry target values: TNT PROTGEO
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Solvent computation | Solvent model: BABINET SCALING / Bsol: 150 Å2 / ksol: 0.7 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.84→20 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Version: 5E / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.185 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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