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- PDB-1apf: ANTHOPLEURIN-B, NMR, 20 STRUCTURES -

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Basic information

Entry
Database: PDB / ID: 1apf
TitleANTHOPLEURIN-B, NMR, 20 STRUCTURES
ComponentsANTHOPLEURIN-B
KeywordsCARDIAC STIMULANT / TOXIN / SEA ANEMONE
Function / homology
Function and homology information


nematocyst / sodium channel regulator activity / regulation of signal transduction / toxin activity / extracellular region
Similarity search - Function
Sea anemone toxin / Anenome neurotoxin / Anthopleurin-A / Myotoxin/Anemone neurotoxin domain superfamily / Anthopleurin-A / Single Sheet / Mainly Beta
Similarity search - Domain/homology
Delta-actitoxin-Axm1b
Similarity search - Component
Biological speciesAnthopleura xanthogrammica (giant green sea anemone)
MethodSOLUTION NMR
AuthorsMonks, S.A. / Pallaghy, P.K. / Scanlon, M.J. / Norton, R.S.
Citation
Journal: Structure / Year: 1995
Title: Solution structure of the cardiostimulant polypeptide anthopleurin-B and comparison with anthopleurin-A.
Authors: Monks, S.A. / Pallaghy, P.K. / Scanlon, M.J. / Norton, R.S.
#1: Journal: Biochemistry / Year: 1995
Title: Three-Dimensional Structure in Solution of the Polypeptide Cardiac Stimulant Anthopleurin-A
Authors: Pallaghy, P.K. / Scanlon, M.J. / Monks, S.A. / Norton, R.S.
#2: Journal: Toxicon / Year: 1991
Title: Structure and Structure-Function Relationships of Sea Anemone Proteins that Interact with the Sodium Channel
Authors: Norton, R.S.
#3: Journal: The Biology of Nematocysts / Year: 1988
Title: Sea Anemone Toxins: Structure and Action
Authors: Kem, W.R.
History
DepositionMay 30, 1995Processing site: BNL
Revision 1.0Jul 11, 1996Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Nov 29, 2017Group: Derived calculations / Other
Category: pdbx_database_status / pdbx_struct_assembly ...pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_conf
Item: _pdbx_database_status.process_site
Revision 1.4Oct 23, 2024Group: Data collection / Database references / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: ANTHOPLEURIN-B


Theoretical massNumber of molelcules
Total (without water)5,2811
Polymers5,2811
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / -
Representative

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Components

#1: Protein/peptide ANTHOPLEURIN-B / AP-B / AXII


Mass: 5281.069 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Anthopleura xanthogrammica (giant green sea anemone)
Organ: WHOLE ANIMAL / References: UniProt: P01531
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Sample conditionspH: 4.5 / Temperature: 298 K
Crystal grow
*PLUS
Method: other / Details: NMR

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Processing

Software
NameVersionClassification
X-PLOR3.1model building
X-PLOR3.1refinement
X-PLOR3.1phasing
NMR softwareName: X-PLOR / Version: 3.1 / Developer: BRUNGER / Classification: refinement
NMR ensembleConformers submitted total number: 20

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