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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1am9 | ||||||
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タイトル | HUMAN SREBP-1A BOUND TO LDL RECEPTOR PROMOTER | ||||||
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![]() | TRANSCRIPTION/DNA / STEROL REGULATORY ELEMENT BINDING PROTEIN / BASIC-HELIX-LOOP-HELIX-LEUCINE ZIPPER / SREBP / TRANSCRIPTION FACTOR / COMPLEX (TRANSCRIPTION REGULATION-DNA) / TRANSCRIPTION-DNA COMPLEX | ||||||
機能・相同性 | ![]() regulation of heart rate by chemical signal / lipid localization / : / sterol response element binding / regulation of protein targeting to mitochondrion / regulation of cholesterol biosynthetic process / regulation of lipid storage / : / SREBP signaling pathway / response to xenobiotic stimulus => GO:0009410 ...regulation of heart rate by chemical signal / lipid localization / : / sterol response element binding / regulation of protein targeting to mitochondrion / regulation of cholesterol biosynthetic process / regulation of lipid storage / : / SREBP signaling pathway / response to xenobiotic stimulus => GO:0009410 / regulation of autophagy of mitochondrion / positive regulation of triglyceride biosynthetic process / chromatin => GO:0000785 / regulation of fatty acid metabolic process / positive regulation of cholesterol biosynthetic process / NR1H2 & NR1H3 regulate gene expression linked to lipogenesis / Regulation of cholesterol biosynthesis by SREBP (SREBF) / response to glucagon / response to food / cellular response to fatty acid / lipid biosynthetic process / response to lipid / fat cell differentiation / negative regulation of insulin secretion / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / response to glucose / response to retinoic acid / response to cAMP / RORA activates gene expression / cellular response to starvation / cholesterol metabolic process / Activation of gene expression by SREBF (SREBP) / response to progesterone / RNA polymerase II transcription regulatory region sequence-specific DNA binding / lung development / mRNA transcription by RNA polymerase II / regulation of protein stability / ER to Golgi transport vesicle membrane / PPARA activates gene expression / lipid metabolic process / positive regulation of miRNA transcription / Transcriptional regulation of white adipocyte differentiation / circadian rhythm / nuclear receptor activity / sequence-specific double-stranded DNA binding / insulin receptor signaling pathway / nuclear envelope / DNA-binding transcription activator activity, RNA polymerase II-specific / response to ethanol / membrane => GO:0016020 / protein dimerization activity / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / Golgi membrane / chromatin binding / endoplasmic reticulum membrane / protein-containing complex binding / regulation of transcription by RNA polymerase II / protein kinase binding / negative regulation of transcription by RNA polymerase II / endoplasmic reticulum / positive regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / nucleoplasm / nucleus / cytosol / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Parraga, A. / Burley, S.K. | ||||||
![]() | ![]() タイトル: Co-crystal structure of sterol regulatory element binding protein 1a at 2.3 A resolution. 著者: Parraga, A. / Bellsolell, L. / Ferre-D'Amare, A.R. / Burley, S.K. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 123.5 KB | 表示 | ![]() |
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PDB形式 | ![]() | 91.9 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: DNA鎖 | 分子量: 5289.417 Da / 分子数: 2 / 由来タイプ: 合成 #2: DNA鎖 | 分子量: 6361.140 Da / 分子数: 2 / 由来タイプ: 合成 #3: タンパク質 | 分子量: 9492.990 Da / 分子数: 4 / Fragment: DNA BINDING DOMAIN / Mutation: C404S / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() #4: 化合物 | #5: 水 | ChemComp-HOH / | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 4.3 Å3/Da / 溶媒含有率: 74.21 % 解説: THE CCD DETECTOR WAS OFFSET IN TWO DIRECTIONS DURING DATA COLLECTION | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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結晶化 | 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 5.6 詳細: PROTEIN WAS CRYSTALLIZED FROM 20 % MPD, 100 MM KCL, 20 MM MGCL2, 100 MM HEPES, PH 5.6, VAPOR DIFFUSION, HANGING DROP | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 |
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結晶化 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | 検出器: CCD / 日付: 1996年10月24日 |
放射 | 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 相対比: 1 |
反射 | 解像度: 2.3→20 Å / Num. obs: 48155 / % possible obs: 87 % / Observed criterion σ(I): 0 / 冗長度: 12 % / Rsym value: 0.071 / Net I/σ(I): 36 |
反射 シェル | 解像度: 2.3→2.48 Å / 冗長度: 4 % / Rsym value: 0.34 / % possible all: 52.4 |
反射 | *PLUS 最高解像度: 2.3 Å / 最低解像度: 20 Å / Num. all: 633236 / % possible obs: 86.9 % / Rmerge(I) obs: 0.071 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: MAX-DNA STRUCTURE 解像度: 2.3→6 Å / σ(F): 2
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精密化ステップ | サイクル: LAST / 解像度: 2.3→6 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS 最高解像度: 2.3 Å / 最低解像度: 6 Å / σ(F): 2 / % reflection Rfree: 10 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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