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- PDB-1a8j: IMMUNOGLOBULIN LAMBDA LIGHT CHAIN DIMER (MCG) COMPLEX WITH ASPARTAME -
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Open data
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Basic information
Entry | Database: PDB / ID: 1a8j | |||||||||
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Title | IMMUNOGLOBULIN LAMBDA LIGHT CHAIN DIMER (MCG) COMPLEX WITH ASPARTAME | |||||||||
![]() | IMMUNOGLOBULIN LAMBDA LIGHT CHAIN DIMER (MCG) | |||||||||
![]() | IMMUNOGLOBULIN / ASPARTAME / LIGAND BINDING / IMMUNOTHERAPEUTIC AGENT / SURROGATE RECEPTOR / MCG(HUMAN) BENCE-JONES DIMER | |||||||||
Function / homology | ![]() CD22 mediated BCR regulation / immunoglobulin complex / Fc epsilon receptor (FCERI) signaling / Classical antibody-mediated complement activation / Initial triggering of complement / FCGR activation / Role of phospholipids in phagocytosis / Role of LAT2/NTAL/LAB on calcium mobilization / Scavenging of heme from plasma / FCERI mediated Ca+2 mobilization ...CD22 mediated BCR regulation / immunoglobulin complex / Fc epsilon receptor (FCERI) signaling / Classical antibody-mediated complement activation / Initial triggering of complement / FCGR activation / Role of phospholipids in phagocytosis / Role of LAT2/NTAL/LAB on calcium mobilization / Scavenging of heme from plasma / FCERI mediated Ca+2 mobilization / FCGR3A-mediated IL10 synthesis / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / Regulation of Complement cascade / Cell surface interactions at the vascular wall / FCERI mediated MAPK activation / FCGR3A-mediated phagocytosis / antigen binding / Regulation of actin dynamics for phagocytic cup formation / FCERI mediated NF-kB activation / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / adaptive immune response / Potential therapeutics for SARS / immune response / extracellular space / extracellular region / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() | |||||||||
![]() | Edmundson, A.B. / Manion, C.V. | |||||||||
![]() | ![]() Title: Treatment of osteoarthritis with aspartame. Authors: Edmundson, A.B. / Manion, C.V. #1: ![]() Title: Three-Dimensional Structure of a Light Chain Dimer Crystallized in Water. Conformational Flexibility of a Molecule in Two Crystal Forms Authors: Ely, K.R. / Herron, J.N. / Harker, M. / Edmundson, A.B. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 78.6 KB | Display | ![]() |
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PDB format | ![]() | 62.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 500.9 KB | Display | ![]() |
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Full document | ![]() | 540.5 KB | Display | |
Data in XML | ![]() | 15.5 KB | Display | |
Data in CIF | ![]() | 21.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 22833.064 Da / Num. of mol.: 2 / Source method: isolated from a natural source Details: FOUND IN THE URINE OF PATIENT MCG WITH A MULTIPLE MYELOMA AND AMYLOIDOSIS Source: (natural) ![]() #2: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.07 Å3/Da / Density % sol: 61 % | ||||||||||||||||||||
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Crystal grow | pH: 6.2 Details: PROTEIN WAS CRYSTALLIZED IN FRESH 1.9M AMMONIUM SULFATE BUFFERED AT PH 6.2 WITH PHOSPHATE AND THE MOTHER LIQUOR WAS SATURATED WITH ASPARTAME. | ||||||||||||||||||||
Crystal grow | *PLUS Method: unknown | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 286 K |
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Diffraction source | Source: SEALED TUBE / Type: OTHER / Wavelength: 1.5418 |
Detector | Type: SIEMENS / Detector: DIFFRACTOMETER / Date: Nov 1, 1988 |
Radiation | Monochromator: NI FILTER / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Highest resolution: 2.7 Å / Num. obs: 10570 / % possible obs: 62 % / Observed criterion σ(I): 2 / Biso Wilson estimate: 17 Å2 / Net I/σ(I): 20 |
Reflection shell | Resolution: 2.7→2.82 Å / Mean I/σ(I) obs: 7 / % possible all: 30 |
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Processing
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Refinement | Method to determine structure: DIFFERENCE MAP / Resolution: 2.7→8 Å / Data cutoff high absF: 1000000 / Data cutoff low absF: 0.001 / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 17 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→8 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.7→2.82 Å / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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