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- PDB-1a7m: LEUKAEMIA INHIBITORY FACTOR CHIMERA (MH35-LIF), NMR, 20 STRUCTURES -

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Basic information

Entry
Database: PDB / ID: 1a7m
TitleLEUKAEMIA INHIBITORY FACTOR CHIMERA (MH35-LIF), NMR, 20 STRUCTURES
ComponentsLEUKEMIA INHIBITORY FACTOR
KeywordsCYTOKINE / FOUR HELICAL BUNDLE
Function / homology
Function and homology information


leukemia inhibitory factor receptor binding / spongiotrophoblast differentiation / meiotic nuclear division / positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis / transdifferentiation / IL-6-type cytokine receptor ligand interactions / negative regulation of meiotic nuclear division / positive regulation of corticotropin secretion / muscle organ morphogenesis / cell surface receptor signaling pathway via STAT ...leukemia inhibitory factor receptor binding / spongiotrophoblast differentiation / meiotic nuclear division / positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis / transdifferentiation / IL-6-type cytokine receptor ligand interactions / negative regulation of meiotic nuclear division / positive regulation of corticotropin secretion / muscle organ morphogenesis / cell surface receptor signaling pathway via STAT / leukemia inhibitory factor signaling pathway / lung vasculature development / regulation of metanephric nephron tubule epithelial cell differentiation / negative regulation of hormone secretion / trophoblast giant cell differentiation / positive regulation of peptidyl-serine phosphorylation of STAT protein / lung lobe morphogenesis / positive regulation of macrophage differentiation / positive regulation of astrocyte differentiation / astrocyte differentiation / positive regulation of cell adhesion mediated by integrin / lung alveolus development / regulation of cell differentiation / somatic stem cell population maintenance / decidualization / neuron development / maternal process involved in female pregnancy / blood vessel remodeling / animal organ regeneration / positive regulation of tyrosine phosphorylation of STAT protein / embryo implantation / negative regulation of angiogenesis / cytokine activity / stem cell differentiation / lung development / growth factor activity / cell morphogenesis / negative regulation of ERK1 and ERK2 cascade / positive regulation of neuron projection development / positive regulation of fibroblast proliferation / positive regulation of peptidyl-tyrosine phosphorylation / retina development in camera-type eye / positive regulation of peptidyl-serine phosphorylation / gene expression / fibroblast proliferation / cell population proliferation / positive regulation of MAPK cascade / response to hypoxia / immune response / negative regulation of cell population proliferation / signaling receptor binding / positive regulation of cell population proliferation / positive regulation of gene expression / positive regulation of transcription by RNA polymerase II / extracellular space / cytosol
Similarity search - Function
Leukemia inhibitory factor / Leukemia inhibitory factor /oncostatin / Leukemia inhibitory factor /oncostatin, conserved site / LIF / OSM family / LIF / OSM family signature. / leukemia inhibitory factor / Growth Hormone; Chain: A; - #10 / Four-helical cytokine-like, core / Growth Hormone; Chain: A; / Up-down Bundle / Mainly Alpha
Similarity search - Domain/homology
Leukemia inhibitory factor
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodSOLUTION NMR / distance geometry
AuthorsHinds, M.G. / Maurer, T. / Zhang, J.-G. / Nicola, N.A. / Norton, R.S.
CitationJournal: J.Biol.Chem. / Year: 1998
Title: Solution structure of leukemia inhibitory factor.
Authors: Hinds, M.G. / Maurer, T. / Zhang, J.G. / Nicola, N.A. / Norton, R.S.
History
DepositionMar 16, 1998Processing site: BNL
Revision 1.0Apr 20, 1999Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 16, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site / _struct_ref_seq_dif.details

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: LEUKEMIA INHIBITORY FACTOR


Theoretical massNumber of molelcules
Total (without water)19,8231
Polymers19,8231
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 1000LEAST RESTRAINT VIOLATION
RepresentativeModel #1

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Components

#1: Protein LEUKEMIA INHIBITORY FACTOR / / LIF


Mass: 19822.939 Da / Num. of mol.: 1 / Mutation: T107S, Q112H, V113S, A155V, R158K
Source method: isolated from a genetically manipulated source
Details: MURINE-HUMAN CHIMERA / Source: (gene. exp.) Mus musculus (house mouse) / Fragment: RESIDUES 48-81 / Production host: Escherichia coli (E. coli) / Strain (production host): NM522 / References: UniProt: P09056

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
11115N-NOESY-HSQC
12115N-TOCSY-HSQC
13113C-NOESY-HSQC
NMR detailsText: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N LABELLED MH35 LEUKAEMIA INHIBITORY FACTOR

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Sample preparation

DetailsContents: H2O
Sample conditionsIonic strength: 1 mM / pH: 4.4 / Pressure: AMBIENT / Temperature: 310 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

NMR spectrometerType: Bruker AMX 600 / Manufacturer: Bruker / Model: AMX 600 / Field strength: 600 MHz

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Processing

Software
NameClassification
DYANAmodel building
X-PLORmodel building
DYANArefinement
X-PLORrefinement
NMR software
NameDeveloperClassification
X-PLORBRUNGERrefinement
DYANAstructure solution
X-PLORstructure solution
RefinementMethod: distance geometry / Software ordinal: 1 / Details: RESTRAINED SIMULATED ANNEALING OF DYANA STRUCTURES
NMR ensembleConformer selection criteria: LEAST RESTRAINT VIOLATION / Conformers calculated total number: 1000 / Conformers submitted total number: 20

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