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Open data
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Basic information
Entry | Database: PDB / ID: 1a6z | ||||||
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Title | HFE (HUMAN) HEMOCHROMATOSIS PROTEIN | ||||||
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![]() | MHC CLASS I COMPLEX / HFE / HEREDITARY HEMOCHROMATOSIS / MHC CLASS I | ||||||
Function / homology | ![]() negative regulation of antigen processing and presentation of endogenous peptide antigen via MHC class I / response to iron ion starvation / negative regulation of T cell cytokine production / hormone biosynthetic process / negative regulation of CD8-positive, alpha-beta T cell activation / co-receptor binding / Transferrin endocytosis and recycling / transferrin receptor binding / regulation of protein localization to cell surface / basal part of cell ...negative regulation of antigen processing and presentation of endogenous peptide antigen via MHC class I / response to iron ion starvation / negative regulation of T cell cytokine production / hormone biosynthetic process / negative regulation of CD8-positive, alpha-beta T cell activation / co-receptor binding / Transferrin endocytosis and recycling / transferrin receptor binding / regulation of protein localization to cell surface / basal part of cell / positive regulation of signaling receptor activity / response to iron ion / positive regulation of peptide hormone secretion / positive regulation of SMAD protein signal transduction / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / beta-2-microglobulin binding / BMP signaling pathway / negative regulation of signaling receptor activity / negative regulation of ubiquitin-dependent protein catabolic process / positive regulation of ferrous iron binding / positive regulation of transferrin receptor binding / positive regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / negative regulation of receptor binding / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / antigen processing and presentation of endogenous peptide antigen via MHC class Ib / cellular response to iron ion / Endosomal/Vacuolar pathway / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / cellular response to iron(III) ion / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / negative regulation of forebrain neuron differentiation / regulation of erythrocyte differentiation / ER to Golgi transport vesicle membrane / peptide antigen assembly with MHC class I protein complex / regulation of iron ion transport / response to molecule of bacterial origin / MHC class I peptide loading complex / HFE-transferrin receptor complex / T cell mediated cytotoxicity / recycling endosome / antigen processing and presentation of endogenous peptide antigen via MHC class I / positive regulation of T cell cytokine production / MHC class I protein complex / multicellular organismal-level iron ion homeostasis / negative regulation of neurogenesis / peptide antigen assembly with MHC class II protein complex / positive regulation of receptor-mediated endocytosis / MHC class II protein complex / cellular response to nicotine / positive regulation of T cell mediated cytotoxicity / specific granule lumen / recycling endosome membrane / phagocytic vesicle membrane / positive regulation of cellular senescence / peptide antigen binding / negative regulation of epithelial cell proliferation / antigen processing and presentation of exogenous peptide antigen via MHC class II / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / Interferon gamma signaling / positive regulation of immune response / Modulation by Mtb of host immune system / sensory perception of smell / positive regulation of T cell activation / positive regulation of protein binding / tertiary granule lumen / apical part of cell / DAP12 signaling / negative regulation of neuron projection development / MHC class II protein complex binding / late endosome membrane / iron ion transport / ER-Phagosome pathway / early endosome membrane / T cell differentiation in thymus / cytoplasmic vesicle / protein refolding / protein-containing complex assembly / protein homotetramerization / intracellular iron ion homeostasis / amyloid fibril formation / learning or memory / early endosome / Amyloid fiber formation / endoplasmic reticulum lumen / lysosomal membrane / external side of plasma membrane / Golgi membrane / focal adhesion / signaling receptor binding / Neutrophil degranulation / positive regulation of gene expression / SARS-CoV-2 activates/modulates innate and adaptive immune responses / structural molecule activity / perinuclear region of cytoplasm / Golgi apparatus / endoplasmic reticulum / protein homodimerization activity Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lebron, J.A. / Bennett, M.J. / Vaughn, D.E. / Chirino, A.J. / Snow, P.M. / Mintier, G.A. / Feder, J.N. / Bjorkman, P.J. | ||||||
![]() | ![]() Title: Crystal structure of the hemochromatosis protein HFE and characterization of its interaction with transferrin receptor. Authors: Lebron, J.A. / Bennett, M.J. / Vaughn, D.E. / Chirino, A.J. / Snow, P.M. / Mintier, G.A. / Feder, J.N. / Bjorkman, P.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 156.2 KB | Display | ![]() |
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PDB format | ![]() | 127.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 393.1 KB | Display | ![]() |
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Full document | ![]() | 419.8 KB | Display | |
Data in XML | ![]() | 17.7 KB | Display | |
Data in CIF | ![]() | 26.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2clrS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 32339.434 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 11748.160 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.89 Å3/Da / Density % sol: 57 % | |||||||||||||||||||||||||
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Crystal grow | pH: 6.5 / Details: pH 6.5 | |||||||||||||||||||||||||
Crystal grow | *PLUS pH: 5.9 / Method: vapor diffusion, hanging dropDetails: drop contained 1:1 mixture of protein and reservoir solution | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 123 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: FUJI / Detector: IMAGE PLATE / Date: Jul 1, 1997 / Details: MIRRORS |
Radiation | Monochromator: SI(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.07 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→15 Å / Num. obs: 187780 / % possible obs: 98 % / Observed criterion σ(I): -3 / Redundancy: 5 % / Biso Wilson estimate: 67.7 Å2 / Rmerge(I) obs: 0.059 / Net I/σ(I): 24 |
Reflection shell | Resolution: 2.6→2.7 Å / Redundancy: 3.5 % / Rmerge(I) obs: 0.421 / Mean I/σ(I) obs: 3 / % possible all: 97 |
Reflection | *PLUS Num. obs: 32145 / Num. measured all: 188780 |
Reflection shell | *PLUS % possible obs: 97 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2CLR Resolution: 2.6→15 Å / Rfactor Rfree error: 0.007 / Data cutoff high absF: 10000000 / Data cutoff low absF: 0.001 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Details: BULK SOLVENT MODEL USED
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Displacement parameters | Biso mean: 63.2 Å2
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Refinement step | Cycle: LAST / Resolution: 2.6→15 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: RESTRAINED / Rms dev Biso : 7.4 Å2 / Rms dev position: 0.02 Å / Weight Biso : 2 / Weight position: 300 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.6→2.71 Å / Rfactor Rfree error: 0.03 / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.399 |