+Open data
-Basic information
Entry | Database: PDB / ID: 1a55 | ||||||
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Title | PHOSPHATE-BINDING PROTEIN MUTANT A197C | ||||||
Components | PHOSPHATE-BINDING PROTEIN | ||||||
Keywords | PHOSPHOTRANSFERASE / TRANSPORT | ||||||
Function / homology | Function and homology information regulation of phosphatase activity / phosphate ion transport / phosphate ion transmembrane transport / phosphate ion binding / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / response to radiation / outer membrane-bounded periplasmic space / DNA damage response / membrane Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / molecular replacement / Resolution: 2.4 Å | ||||||
Authors | Hirshberg, M. / Henrick, K. / Lloyd-Haire, L. / Vasisht, N. / Brune, M. / Corrie, J.E.T. / Webb, M.R. | ||||||
Citation | Journal: Biochemistry / Year: 1998 Title: Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate. Authors: Hirshberg, M. / Henrick, K. / Haire, L.L. / Vasisht, N. / Brune, M. / Corrie, J.E. / Webb, M.R. #1: Journal: Biochemistry / Year: 1998 Title: Mechanism of Inorganic Phosphate Interaction with Phosphate Binding Protein from Escherichia Coli Authors: Brune, M. / Hunter, J.L. / Howell, S.A. / Martin, S.R. / Hazlett, T.L. / Corrie, J.E. / Webb, M.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1a55.cif.gz | 79.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1a55.ent.gz | 59.3 KB | Display | PDB format |
PDBx/mmJSON format | 1a55.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1a55_validation.pdf.gz | 384.2 KB | Display | wwPDB validaton report |
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Full document | 1a55_full_validation.pdf.gz | 394.6 KB | Display | |
Data in XML | 1a55_validation.xml.gz | 9.4 KB | Display | |
Data in CIF | 1a55_validation.cif.gz | 15.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a5/1a55 ftp://data.pdbj.org/pub/pdb/validation_reports/a5/1a55 | HTTPS FTP |
-Related structure data
Related structure data | 1a54C 2abhS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 34489.664 Da / Num. of mol.: 1 / Mutation: A197C Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: PAN92 / Cell line: AN2538 / Cellular location: PERIPLASM / Gene: PHO-S / Cell line (production host): AN2538 / Cellular location (production host): PERIPLASM / Production host: Escherichia coli (E. coli) / Strain (production host): PIBI24 / References: UniProt: P06128, UniProt: P0AG82*PLUS |
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#2: Chemical | ChemComp-2HP / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.43 Å3/Da / Density % sol: 49.39 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 4.5 / Details: pH 4.5 | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.6 / Method: vapor diffusion, hanging drop / Details: used to seeding | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: Aug 1, 1997 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→25.4 Å / Num. obs: 70788 / % possible obs: 86.6 % / Observed criterion σ(I): 0 / Redundancy: 2.9 % / Rmerge(I) obs: 0.047 |
Reflection shell | Highest resolution: 2.4 Å / Redundancy: 2.1 % / Rmerge(I) obs: 0.19 / % possible all: 69.2 |
Reflection | *PLUS Num. obs: 10913 / Num. measured all: 70788 |
Reflection shell | *PLUS % possible obs: 69.2 % / Mean I/σ(I) obs: 4.2 |
-Processing
Software |
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Refinement | Method to determine structure: molecular replacement Starting model: PDB ENTRY 2ABH Resolution: 2.4→12 Å / Cross valid method: THROUGHOUT
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Refinement step | Cycle: LAST / Resolution: 2.4→12 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.173 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 20.2 Å2 |