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Structure paper

TitleCrystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate.
Journal, issue, pagesBiochemistry, Vol. 37, Page 10381-10385, Year 1998
Publish dateFeb 19, 1998 (structure data deposition date)
AuthorsHirshberg, M. / Henrick, K. / Haire, L.L. / Vasisht, N. / Brune, M. / Corrie, J.E. / Webb, M.R.
External linksBiochemistry / PubMed:9671506
MethodsX-ray diffraction
Resolution1.6 - 2.4 Å
Structure data

PDB-1a54:
PHOSPHATE-BINDING PROTEIN MUTANT A197C LABELLED WITH A COUMARIN FLUOROPHORE AND BOUND TO DIHYDROGENPHOSPHATE ION
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-1a55:
PHOSPHATE-BINDING PROTEIN MUTANT A197C
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

Chemicals

ChemComp-2HP:
DIHYDROGENPHOSPHATE ION

ChemComp-MDC:
N-[2-(1-MALEIMIDYL)ETHYL]-7-DIETHYLAMINOCOUMARIN-3-CARBOXAMIDE

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
KeywordsPHOSPHOTRANSFERASE / TRANSPORT / COUMARIN / FLUOROPHORE

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