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- PDB-1a54: PHOSPHATE-BINDING PROTEIN MUTANT A197C LABELLED WITH A COUMARIN F... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1a54 | |||||||||
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Title | PHOSPHATE-BINDING PROTEIN MUTANT A197C LABELLED WITH A COUMARIN FLUOROPHORE AND BOUND TO DIHYDROGENPHOSPHATE ION | |||||||||
![]() | Phosphate-binding protein PstS | |||||||||
![]() | PHOSPHOTRANSFERASE / TRANSPORT / COUMARIN / FLUOROPHORE | |||||||||
Function / homology | ![]() phosphate ion transport / phosphate ion transmembrane transport / phosphate ion binding / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / ATP-binding cassette (ABC) transporter complex / response to radiation / outer membrane-bounded periplasmic space / DNA damage response / membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Hirshberg, M. / Henrick, K. / Lloyd-Haire, L. / Vasisht, N. / Brune, M. / Corrie, J.E.T. / Webb, M.R. | |||||||||
![]() | ![]() Title: Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate. Authors: Hirshberg, M. / Henrick, K. / Haire, L.L. / Vasisht, N. / Brune, M. / Corrie, J.E. / Webb, M.R. #1: ![]() Title: Mechanism of Inorganic Phosphate Interaction with Phosphate Binding Protein from Escherichia Coli Authors: Brune, M. / Hunter, J.L. / Howell, S.A. / Martin, S.R. / Hazlett, T.L. / Corrie, J.E. / Webb, M.R. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 77.9 KB | Display | ![]() |
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PDB format | ![]() | 60.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 471.9 KB | Display | ![]() |
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Full document | ![]() | 475.5 KB | Display | |
Data in XML | ![]() | 8.6 KB | Display | |
Data in CIF | ![]() | 14.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1a55C ![]() 2abhS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 34489.664 Da / Num. of mol.: 1 / Mutation: A197C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-2HP / |
#3: Chemical | ChemComp-MDC / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 46.74 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 4.5 / Details: pH 4.5 | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.6 / Method: vapor diffusion, hanging drop / Details: used to seeding | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 102 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Aug 1, 1997 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→12 Å / Num. obs: 345731 / % possible obs: 95.5 % / Observed criterion σ(I): 0 / Redundancy: 3.3 % / Rmerge(I) obs: 0.08 |
Reflection shell | Highest resolution: 1.6 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.21 / % possible all: 93.4 |
Reflection | *PLUS Num. obs: 40945 / Num. measured all: 345731 |
Reflection shell | *PLUS % possible obs: 93.4 % / Mean I/σ(I) obs: 3.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2ABH Resolution: 1.6→12 Å / Cross valid method: THROUGHOUT
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Refinement step | Cycle: LAST / Resolution: 1.6→12 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.177 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 14.9 Å2 |