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Yorodumi- PDB-1a03: THE THREE-DIMENSIONAL STRUCTURE OF CA2+-BOUND CALCYCLIN: IMPLICAT... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1a03 | ||||||
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Title | THE THREE-DIMENSIONAL STRUCTURE OF CA2+-BOUND CALCYCLIN: IMPLICATIONS FOR CA2+-SIGNAL TRANSDUCTION BY S100 PROTEINS, NMR, 20 STRUCTURES | ||||||
Components | CALCYCLIN (RABBIT, CA2+) | ||||||
Keywords | CALCIUM-BINDING PROTEIN / EF-HAND / S-100 PROTEIN | ||||||
Function / homology | Function and homology information cytoplasmic side of plasma membrane / nuclear envelope / calcium ion binding / cytosol Similarity search - Function | ||||||
Biological species | Oryctolagus cuniculus (rabbit) | ||||||
Method | SOLUTION NMR / METRIC MATRIX DISTANCE GEOMETRY, , RESTRAINED MOLECULAR DYNAMICS SIMULATED ANNEALING | ||||||
Authors | Sastry, M. / Ketchem, R.R. / Crescenzi, O. / Weber, C. / Lubienski, M.J. / Hidaka, H. / Chazin, W.J. | ||||||
Citation | Journal: Structure / Year: 1998 Title: The three-dimensional structure of Ca(2+)-bound calcyclin: implications for Ca(2+)-signal transduction by S100 proteins. Authors: Sastry, M. / Ketchem, R.R. / Crescenzi, O. / Weber, C. / Lubienski, M.J. / Hidaka, H. / Chazin, W.J. #1: Journal: Protein Sci. / Year: 1996 Title: 1H NMR Assignments of Apo Calcyclin and Comparative Structural Analysis with Calbindin D9K and S100 Beta Authors: Potts, B.C. / Carlstrom, G. / Okazaki, K. / Hidaka, H. / Chazin, W.J. #2: Journal: Nat.Struct.Biol. / Year: 1995 Title: The Structure of Calcyclin Reveals a Novel Homodimeric Fold for S100 Ca(2+)-Binding Proteins Authors: Potts, B.C. / Smith, J. / Akke, M. / Macke, T.J. / Okazaki, K. / Hidaka, H. / Case, D.A. / Chazin, W.J. #3: Journal: Nat.Struct.Biol. / Year: 1995 Title: Erratum. The Structure of Calcyclin Reveals a Novel Homodimeric Fold for S100 Ca(2+)-Binding Proteins Authors: Potts, B.C. / Smith, J. / Akke, M. / Macke, T.J. / Okazaki, K. / Hidaka, H. / Case, D.A. / Chazin, W.J. #4: Journal: Arch.Biochem.Biophys. / Year: 1991 Title: A Calcium-Binding Protein from Rabbit Lung Cytosol Identified as the Product of Growth-Regulated Gene (2A9) and its Binding Proteins Authors: Tokumitsu, H. / Kobayashi, R. / Hidaka, H. #5: Journal: Arch.Biochem.Biophys. / Year: 1991 Title: Erratum. A Calcium-Binding Protein from Rabbit Lung Cytosol Identified as the Product of Growth-Regulated Gene (2A9) and its Binding Proteins Authors: Tokumitsu, H. / Kobayashi, R. / Hidaka, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1a03.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1a03.ent.gz | 962.7 KB | Display | PDB format |
PDBx/mmJSON format | 1a03.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a0/1a03 ftp://data.pdbj.org/pub/pdb/validation_reports/a0/1a03 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10167.729 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: RABBIT, CA2+ / Source: (gene. exp.) Oryctolagus cuniculus (rabbit) / Cell line: BL21 / Gene: R-S100A6 / Organ: LUNG / Plasmid: PET1120 / Cell line (production host): BL21 / Cellular location (production host): CYTOPLASM / Gene (production host): R-S100A6 / Production host: Escherichia coli (E. coli) / Strain (production host): DE3 / References: UniProt: P30801 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: SEE MANUSCRIPT |
-Sample preparation
Details | Contents: 95% H2O, 5% D2O |
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Sample conditions | Ionic strength: 50mM TRIS, 20-38mM CACL2 / pH: 7.0 / Temperature: 300 K |
Crystal grow | *PLUS Method: other |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: METRIC MATRIX DISTANCE GEOMETRY, , RESTRAINED MOLECULAR DYNAMICS SIMULATED ANNEALING Software ordinal: 1 | ||||||||||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATION ENERGY Conformers calculated total number: 192 / Conformers submitted total number: 20 |