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- EMDB-9918: Cryo-EM structure of the tetrameric photosystem I from a heterocy... -

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Basic information

Entry
Database: EMDB / ID: EMD-9918
TitleCryo-EM structure of the tetrameric photosystem I from a heterocyst-forming cyanobacterium Anabaena sp. PCC7120
Map data
Sample
  • Organelle or cellular component: Photosystem I complex of cyanobacterium Anabaena sp. PCC 7120
    • Protein or peptide: x 12 types
  • Ligand: x 8 types
KeywordsPhotosystem I / PSI / PCC7120 / PHOTOSYNTHESIS
Function / homology
Function and homology information


photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosystem I / chlorophyll binding / plasma membrane-derived thylakoid membrane / photosynthesis / 4 iron, 4 sulfur cluster binding / oxidoreductase activity / electron transfer activity ...photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosystem I / chlorophyll binding / plasma membrane-derived thylakoid membrane / photosynthesis / 4 iron, 4 sulfur cluster binding / oxidoreductase activity / electron transfer activity / magnesium ion binding / metal ion binding
Similarity search - Function
Photosystem I PsaX / Photosystem I PsaX superfamily / PsaX family / Photosystem I reaction center subunit PsaK / Photosystem I reaction centre subunit PsaK / Photosystem I PsaM, reaction centre superfamily / Photosystem I PsaM, reaction centre / Photosystem I protein M (PsaM) / Photosystem I reaction centre subunit PsaK superfamily / Photosystem I reaction center subunit V/PsaK ...Photosystem I PsaX / Photosystem I PsaX superfamily / PsaX family / Photosystem I reaction center subunit PsaK / Photosystem I reaction centre subunit PsaK / Photosystem I PsaM, reaction centre superfamily / Photosystem I PsaM, reaction centre / Photosystem I protein M (PsaM) / Photosystem I reaction centre subunit PsaK superfamily / Photosystem I reaction center subunit V/PsaK / Photosystem I psaG / psaK / Photosystem I PsaL, reaction centre subunit XI / Photosystem I, reaction centre subunit XI / Photosystem I PsaL, reaction centre subunit XI superfamily / Photosystem I reaction centre subunit XI / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII superfamily / Photosystem I PsaF, reaction centre subunit III / Photosystem I PsaF, reaction centre subunit III superfamily / Photosystem I reaction centre subunit III / Photosystem I PsaD / Photosystem I, reaction centre subunit PsaD superfamily / PsaD / Photosystem I PsaE, reaction centre subunit IV / Photosystem I PsaJ, reaction centre subunit IX superfamily / Photosystem I reaction centre subunit IV / PsaE / Photosystem I PsaJ, reaction centre subunit IX / Photosystem I reaction centre subunit IX / PsaJ / Photosystem I protein PsaC / Photosystem I PsaA / Photosystem I PsaB / Photosystem I PsaA/PsaB, conserved site / Photosystem I psaA and psaB proteins signature. / : / Photosystem I PsaA/PsaB / Photosystem I PsaA/PsaB superfamily / Photosystem I psaA/psaB protein / Electron transport accessory-like domain superfamily / 4Fe-4S dicluster domain / 4Fe-4S ferredoxin, iron-sulphur binding, conserved site / 4Fe-4S ferredoxin-type iron-sulfur binding region signature. / 4Fe-4S ferredoxin-type iron-sulfur binding domain profile. / 4Fe-4S ferredoxin-type, iron-sulphur binding domain
Similarity search - Domain/homology
Photosystem I iron-sulfur center / Photosystem I reaction center subunit VIII / Photosystem I reaction center subunit III / Photosystem I P700 chlorophyll a apoprotein A2 1 / Photosystem I 4.8 kDa protein / Photosystem I reaction center subunit IX / Photosystem I reaction center subunit II / Photosystem I reaction center subunit IV / Photosystem I P700 chlorophyll a apoprotein A1 / Photosystem I reaction center subunit XI ...Photosystem I iron-sulfur center / Photosystem I reaction center subunit VIII / Photosystem I reaction center subunit III / Photosystem I P700 chlorophyll a apoprotein A2 1 / Photosystem I 4.8 kDa protein / Photosystem I reaction center subunit IX / Photosystem I reaction center subunit II / Photosystem I reaction center subunit IV / Photosystem I P700 chlorophyll a apoprotein A1 / Photosystem I reaction center subunit XI / Photosystem I reaction center subunit PsaK 1 / Photosystem I reaction center subunit XII
Similarity search - Component
Biological speciesNostoc sp. PCC 7120 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.37 Å
AuthorsZheng L / Li Y
Funding support1 items
OrganizationGrant numberCountry
National Science Foundation (China)
CitationJournal: Nat Plants / Year: 2019
Title: Structural and functional insights into the tetrameric photosystem I from heterocyst-forming cyanobacteria.
Authors: Lvqin Zheng / Yanbing Li / Xiying Li / Qinglu Zhong / Ningning Li / Kun Zhang / Yuebin Zhang / Huiying Chu / Chengying Ma / Guohui Li / Jindong Zhao / Ning Gao /
Abstract: Two large protein-cofactor complexes, photosystem I and photosystem II, are the central components of photosynthesis in the thylakoid membranes. Here, we report the 2.37-Å structure of a tetrameric ...Two large protein-cofactor complexes, photosystem I and photosystem II, are the central components of photosynthesis in the thylakoid membranes. Here, we report the 2.37-Å structure of a tetrameric photosystem I complex from a heterocyst-forming cyanobacterium Anabaena sp. PCC 7120. Four photosystem I monomers, organized in a dimer of dimer, form two distinct interfaces that are largely mediated by specifically orientated polar lipids, such as sulfoquinovosyl diacylglycerol. The structure depicts a more closely connected network of chlorophylls across monomer interfaces than those seen in trimeric PSI from thermophilic cyanobacteria, possibly allowing a more efficient energy transfer between monomers. Our physiological data also revealed a functional link of photosystem I oligomerization to cyclic electron flow and thylakoid membrane organization.
History
DepositionMay 31, 2019-
Header (metadata) releaseOct 9, 2019-
Map releaseOct 9, 2019-
UpdateMar 27, 2024-
Current statusMar 27, 2024Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6k61
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6k61
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_9918.png

Downloads & links

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Map

FileDownload / File: emd_9918.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.05 Å/pix.
x 400 pix.
= 420.8 Å		1.05 Å/pix.
x 400 pix.
= 420.8 Å		1.05 Å/pix.
x 400 pix.
= 420.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.052 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.019 / Movie #1: 0.025
Minimum - Maximum-0.07334861 - 0.17260951
Average (Standard dev.)-0.00004018816 (±0.007173404)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 420.80002 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0521.0521.052
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z420.800420.800420.800
α/β/γ90.00090.00090.000
start NX/NY/NZ929262
NX/NY/NZ290290360
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-0.0730.173-0.000

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Supplemental data

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Sample components

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Entire : Photosystem I complex of cyanobacterium Anabaena sp. PCC 7120

EntireName: Photosystem I complex of cyanobacterium Anabaena sp. PCC 7120
Components
  • Organelle or cellular component: Photosystem I complex of cyanobacterium Anabaena sp. PCC 7120
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A1
    • Protein or peptide: Photosystem I 4.8 kDa protein
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A2 1
    • Protein or peptide: Photosystem I iron-sulfur center
    • Protein or peptide: Photosystem I reaction center subunit II
    • Protein or peptide: Photosystem I reaction center subunit IV
    • Protein or peptide: Photosystem I reaction center subunit III
    • Protein or peptide: Photosystem I reaction center subunit IX
    • Protein or peptide: Photosystem I reaction center subunit PsaK 1
    • Protein or peptide: Photosystem I reaction center subunit VIII
    • Protein or peptide: Photosystem I reaction center subunit XI
    • Protein or peptide: Photosystem I reaction center subunit XII
  • Ligand: CHLOROPHYLL A ISOMER
  • Ligand: CHLOROPHYLL A
  • Ligand: PHYLLOQUINONE
  • Ligand: IRON/SULFUR CLUSTER
  • Ligand: BETA-CAROTENE
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

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Supramolecule #1: Photosystem I complex of cyanobacterium Anabaena sp. PCC 7120

SupramoleculeName: Photosystem I complex of cyanobacterium Anabaena sp. PCC 7120
type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1-#12
Source (natural)Organism: Nostoc sp. PCC 7120 (bacteria)
Molecular weightTheoretical: 1.3 MDa

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Macromolecule #1: Photosystem I P700 chlorophyll a apoprotein A1

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A1 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Nostoc sp. PCC 7120 (bacteria) / Strain: PCC 7120
Molecular weightTheoretical: 83.28968 KDa
SequenceString: MTISPPEREE KKARVIVDKD PVPTSFEKWA QPGHFDRTLA RGPKTTTWIW NLHALAHDFD THTSDLEDIS RKIFAAHFGH LAVVTIWLS GMIFHGAKFS NYEAWLSDPL NVRPSAQVVW PIVGQDILNG DVGGGFHGIQ ITSGLFQVWR GWGITNSFQL Y CTAIGGLV ...String:
MTISPPEREE KKARVIVDKD PVPTSFEKWA QPGHFDRTLA RGPKTTTWIW NLHALAHDFD THTSDLEDIS RKIFAAHFGH LAVVTIWLS GMIFHGAKFS NYEAWLSDPL NVRPSAQVVW PIVGQDILNG DVGGGFHGIQ ITSGLFQVWR GWGITNSFQL Y CTAIGGLV LAGLFLFAGW FHYHKRAPKL EWFQNVESML NHHLQVLLGC GSLGWAGHLI HVSAPINKLM DAGVAVKDIP LP HEFILNK SLLIDLFPGF AAGLTPFFTL NWGQYADFLT FKGGLNPVTG GLWMTDIAHH HLAIAVVFII AGHQYRTNWG IGH SIKEIL ENHKGPFTGE GHKGLYENLT TSWHAQLATN LAFLGSLTII IAHHMYAMPP YPYLATDYAT QLCIFTHHIW IGGF LIVGG AAHAAIFMVR DYDPVVNQNN VLDRVIRHRD AIISHLNWVC IFLGFHSFGL YIHNDTMRAL GRPQDMFSDT AIQLQ PVFA QWVQNLHTLA PGGTAPNALE PVSYAFGGGV LAVGGKVAMM PIALGTADFL IHHIHAFTIH VTVLILLKGV LFARSS RLI PDKANLGFRF PCDGPGRGGT CQVSGWDHVF LGLFWMYNSL SIVIFHFSWK MQSDVWGTVD AAGNVSHITG GNFAQSA IT INGWLRDFLW AQASQVINSY GSALSAYGLM FLGAHFVWAF SLMFLFSGRG YWQELIESIV WAHNKLKVAP AIQPRALS I TQGRAVGVAH YLLGGIATTW AFFHAHILSV G

UniProtKB: Photosystem I P700 chlorophyll a apoprotein A1

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Macromolecule #2: Photosystem I 4.8 kDa protein

MacromoleculeName: Photosystem I 4.8 kDa protein / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 (bacteria) / Strain: PCC 7120
Molecular weightTheoretical: 4.872792 KDa
SequenceString:
MAKAKISPVA NTGAKPPYTF RTGWALLLLA VNFLVAAYYF HIIQ

UniProtKB: Photosystem I 4.8 kDa protein

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Macromolecule #3: Photosystem I P700 chlorophyll a apoprotein A2 1

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A2 1 / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Nostoc sp. PCC 7120 (bacteria) / Strain: PCC 7120
Molecular weightTheoretical: 83.457016 KDa
SequenceString: MATKFPKFSQ DLAQDPTTRR IWYAMAMGND FESHDGMTEE NLYQKIFATH FGHLAIIFLW ASSLLFHVAW QGNFEQWIKD PLHVRPIAH AIWDPHFGKP AIEAFTQAGA NGPVNIAYSG VYHWWYTIGM RTNTELYTGS VFLLLFASLF LFAGWLHLQP K FRPSLAWF ...String:
MATKFPKFSQ DLAQDPTTRR IWYAMAMGND FESHDGMTEE NLYQKIFATH FGHLAIIFLW ASSLLFHVAW QGNFEQWIKD PLHVRPIAH AIWDPHFGKP AIEAFTQAGA NGPVNIAYSG VYHWWYTIGM RTNTELYTGS VFLLLFASLF LFAGWLHLQP K FRPSLAWF KSAESRLNHH LAGLFGVSSL AWAGHLIHVA IPESRGQHVG WDNFLSTAPH PAGLQPFFTG NWGVYAQNPD TA GHIFSTS QGAGTAILTF LGGFHPQTES LWLTDMAHHH LAIAVLFIVA GHMYRTNFGI GHSIKEMMNA KTFFGKPVEG PFN MPHQGI YDTYNNSLHF QLGWHLACLG VVTSWVAQHM YSLPSYAFIA KDYTTQAALY THHQYIAIFL MVGAFAHGAI FLVR DYDPE QNKGNVLERV LQHKEAIISH LSWVSLFLGF HTLGLYVHND VVVAFGTPEK QILIEPVFAQ FIQAAHGKVL YGLDT LLSN PDSVAYTAYP NYANVWLPGW LDAINSGTNS LFLTIGPGDF LVHHAIALGL HTTTLILVKG ALDARGSKLM PDKKDF GYA FPCDGPGRGG TCDISAWDSF YLSLFWALNT VGWVTFYWHW KHLGIWQGNV AQFNENSTYL MGWFRDYLWA NSAQLIN GY NPYGVNNLSV WAWMFLFGHL VWATGFMFLI SWRGYWQELI ETLVWAHERT PIANLVRWKD KPVALSIVQA RVVGLAHF T VGYVLTYAAF LIASTAGKFG

UniProtKB: Photosystem I P700 chlorophyll a apoprotein A2 1

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Macromolecule #4: Photosystem I iron-sulfur center

MacromoleculeName: Photosystem I iron-sulfur center / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Nostoc sp. PCC 7120 (bacteria) / Strain: PCC 7120
Molecular weightTheoretical: 8.825206 KDa
SequenceString:
MSHTVKIYDT CIGCTQCVRA CPTDVLEMVP WDGCKAAQVA SSPRTEDCVG CKRCETACPT DFLSIRVYLG AETTRSMGLA Y

UniProtKB: Photosystem I iron-sulfur center

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Macromolecule #5: Photosystem I reaction center subunit II

MacromoleculeName: Photosystem I reaction center subunit II / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 (bacteria) / Strain: PCC 7120
Molecular weightTheoretical: 15.17624 KDa
SequenceString:
MAETLSGKTP LFAGSTGGLL TKAVEEEKYA ITWTSPKAQV FELPTGGAAT MHEGENLLYI ARKEYGIALG GQLRKFKITN YKIYRILPS GETTFIHPAD GVFPEKVNAG REKVRFNARS IGENPNPSQV KFSGKATYDA

UniProtKB: Photosystem I reaction center subunit II

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Macromolecule #6: Photosystem I reaction center subunit IV

MacromoleculeName: Photosystem I reaction center subunit IV / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 (bacteria) / Strain: PCC 7120
Molecular weightTheoretical: 7.897051 KDa
SequenceString:
MVQRGSKVRI LRPESYWFQD VGTVASVDQS GIKYPVIVRF DKVNYAGINT NNFAVDELIE VEAPKAKAKK

UniProtKB: Photosystem I reaction center subunit IV

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Macromolecule #7: Photosystem I reaction center subunit III

MacromoleculeName: Photosystem I reaction center subunit III / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 (bacteria) / Strain: PCC 7120
Molecular weightTheoretical: 17.850568 KDa
SequenceString:
MRRLFALILV ICLSFSFAPP AKALGADLTP CAENPAFQAL AKNARNTTAD PQSGQKRFER YSQALCGPEG YPHLIVDGRL DRAGDFLIP SILFLYIAGW IGWVGRAYLQ AIKKDSDTEQ KEIQLDLGIA LPIIATGFAW PAAAVKELLS GELTAKDSEI T VSPR

UniProtKB: Photosystem I reaction center subunit III

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Macromolecule #8: Photosystem I reaction center subunit IX

MacromoleculeName: Photosystem I reaction center subunit IX / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 (bacteria) / Strain: PCC 7120
Molecular weightTheoretical: 5.499422 KDa
SequenceString:
MADKADQSSY LIKFISTAPV AATIWLTITA GILIEFNRFF PDLLFHPLP

UniProtKB: Photosystem I reaction center subunit IX

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Macromolecule #9: Photosystem I reaction center subunit PsaK 1

MacromoleculeName: Photosystem I reaction center subunit PsaK 1 / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 (bacteria) / Strain: PCC 7120
Molecular weightTheoretical: 8.852457 KDa
SequenceString:
MLTSTLLAAA TTPLEWSPTV GIIMVIANVI AITFGRQTIK YPSAEPALPS AKFFGGFGAP ALLATTAFGH ILGVGLVLGL HNLGRI

UniProtKB: Photosystem I reaction center subunit PsaK 1

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Macromolecule #10: Photosystem I reaction center subunit VIII

MacromoleculeName: Photosystem I reaction center subunit VIII / type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 (bacteria) / Strain: PCC 7120
Molecular weightTheoretical: 5.066925 KDa
SequenceString:
MATAFLPSIL ADASFLSSIF VPVIGWVVPI ATFSFLFLYI EREDVA

UniProtKB: Photosystem I reaction center subunit VIII

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Macromolecule #11: Photosystem I reaction center subunit XI

MacromoleculeName: Photosystem I reaction center subunit XI / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 (bacteria) / Strain: PCC 7120
Molecular weightTheoretical: 18.130725 KDa
SequenceString:
MAQAVDASKN LPSDPRNREV VFPAGRDPQW GNLETPVNAS PLVKWFINNL PAYRPGLTPF RRGLEVGMAH GYFLFGPFAK LGPLRDAAN ANLAGLLGAI GLVVLFTLAL SLYANSNPPT ALASVTVPNP PDAFQSKEGW NNFASAFLIG GIGGAVVAYF L TSNLALIQ GLVG

UniProtKB: Photosystem I reaction center subunit XI

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Macromolecule #12: Photosystem I reaction center subunit XII

MacromoleculeName: Photosystem I reaction center subunit XII / type: protein_or_peptide / ID: 12 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 (bacteria) / Strain: PCC 7120
Molecular weightTheoretical: 3.538204 KDa
SequenceString:
MSSISDTQVY IALVVALIPG LLAWRLATEL YK

UniProtKB: Photosystem I reaction center subunit XII

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Macromolecule #13: CHLOROPHYLL A ISOMER

MacromoleculeName: CHLOROPHYLL A ISOMER / type: ligand / ID: 13 / Number of copies: 2 / Formula: CL0
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CL0:
CHLOROPHYLL A ISOMER

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Macromolecule #14: CHLOROPHYLL A

MacromoleculeName: CHLOROPHYLL A / type: ligand / ID: 14 / Number of copies: 188 / Formula: CLA
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CLA:
CHLOROPHYLL A

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Macromolecule #15: PHYLLOQUINONE

MacromoleculeName: PHYLLOQUINONE / type: ligand / ID: 15 / Number of copies: 4 / Formula: PQN
Molecular weightTheoretical: 450.696 Da
Chemical component information

ChemComp-PQN:
PHYLLOQUINONE

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Macromolecule #16: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 16 / Number of copies: 6 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

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Macromolecule #17: BETA-CAROTENE

MacromoleculeName: BETA-CAROTENE / type: ligand / ID: 17 / Number of copies: 46 / Formula: BCR
Molecular weightTheoretical: 536.873 Da
Chemical component information

ChemComp-BCR:
BETA-CAROTENE

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Macromolecule #18: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 18 / Number of copies: 6 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

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Macromolecule #19: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

MacromoleculeName: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
type: ligand / ID: 19 / Number of copies: 4 / Formula: SQD
Molecular weightTheoretical: 795.116 Da
Chemical component information

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

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Macromolecule #20: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 20 / Number of copies: 3 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statehelical array

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Sample preparation

BufferpH: 6.5
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 58.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

1jb0

Final reconstructionApplied symmetry - Point group: C2 (2 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 2.37 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 71600
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: PROJECTION MATCHING

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Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

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