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- EMDB-9905: RSC substrate-recruitment module -

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Basic information

Entry
Database: EMDB / ID: EMD-9905
TitleRSC substrate-recruitment module
Map dataRSC SRM region
Sample
  • Complex: RSC
    • Protein or peptide: x 12 types
  • Ligand: x 1 types
Keywordschromatin remodeler / SWI/SNF family / DNA BINDING PROTEIN
Function / homology
Function and homology information


regulation of sporulation resulting in formation of a cellular spore / chromatin remodeling at centromere / histone H4 reader activity / regulation of nuclear cell cycle DNA replication / plasmid maintenance / DNA translocase activity / nucleosome array spacer activity / RSC-type complex / ATP-dependent chromatin remodeler activity / UV-damage excision repair ...regulation of sporulation resulting in formation of a cellular spore / chromatin remodeling at centromere / histone H4 reader activity / regulation of nuclear cell cycle DNA replication / plasmid maintenance / DNA translocase activity / nucleosome array spacer activity / RSC-type complex / ATP-dependent chromatin remodeler activity / UV-damage excision repair / nucleosome disassembly / SWI/SNF complex / sister chromatid cohesion / sporulation resulting in formation of a cellular spore / nuclear chromosome / rRNA transcription / chromosome, centromeric region / nucleosome binding / cytoskeleton organization / meiotic cell cycle / : / helicase activity / positive regulation of transcription elongation by RNA polymerase II / chromosome segregation / transcription coregulator activity / transcription elongation by RNA polymerase II / double-strand break repair via homologous recombination / base-excision repair / chromatin DNA binding / double-strand break repair via nonhomologous end joining / G2/M transition of mitotic cell cycle / double-strand break repair / DNA helicase / histone binding / sequence-specific DNA binding / forked DNA-dependent helicase activity / single-stranded 3'-5' DNA helicase activity / four-way junction helicase activity / double-stranded DNA helicase activity / DNA-binding transcription factor activity, RNA polymerase II-specific / chromatin remodeling / chromatin binding / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / chromatin / positive regulation of DNA-templated transcription / structural molecule activity / positive regulation of transcription by RNA polymerase II / ATP hydrolysis activity / DNA binding / zinc ion binding / ATP binding / nucleus
Similarity search - Function
: / Rsc1/Rsc2, bromodomain / : / Rsc8/Ssr1/Ssr2, zinc finger, ZZ-type / : / RSC4 Ig-like domain / Chromatin-remodelling complex, RSC SWI/SNF subunit Rsc7/Swp82 / Chromatin remodelling complex Rsc7/Swp82 subunit / DNA-binding RFX-type winged-helix domain / RFX-type winged-helix DNA-binding domain profile. ...: / Rsc1/Rsc2, bromodomain / : / Rsc8/Ssr1/Ssr2, zinc finger, ZZ-type / : / RSC4 Ig-like domain / Chromatin-remodelling complex, RSC SWI/SNF subunit Rsc7/Swp82 / Chromatin remodelling complex Rsc7/Swp82 subunit / DNA-binding RFX-type winged-helix domain / RFX-type winged-helix DNA-binding domain profile. / : / Chromatin-remodeling complex component Sfh1/SNF5 / SMARCC, C-terminal / SWIRM-associated region 1 / SNF5/SMARCB1/INI1 / SNF5 / SMARCB1 / INI1 / Remodelling complex subunit Rsc/polybromo / Zn(2)-C6 fungal-type DNA-binding domain signature. / Fungal Zn(2)-Cys(6) binuclear cluster domain / Zn(2)-C6 fungal-type DNA-binding domain superfamily / Zn(2)-C6 fungal-type DNA-binding domain profile. / GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain / Zn(2)-C6 fungal-type DNA-binding domain / Snf2-ATP coupling, chromatin remodelling complex / Snf2, ATP coupling domain / Snf2-ATP coupling, chromatin remodelling complex / HSA domain / Helicase/SANT-associated domain / HSA domain profile. / SWIRM domain / SWIRM domain / SWIRM domain profile. / Bromo adjacent homology domain / BAH domain / Bromo adjacent homology (BAH) domain / Bromo adjacent homology (BAH) domain superfamily / BAH domain profile. / SWIB/MDM2 domain superfamily / SANT domain profile. / SANT domain / Zinc-binding domain, present in Dystrophin, CREB-binding protein. / Zinc finger, ZZ type / Zinc finger, ZZ-type / Zinc finger, ZZ-type superfamily / Zinc finger ZZ-type profile. / Myb-like DNA-binding domain / : / SNF2-like, N-terminal domain superfamily / SNF2, N-terminal / SNF2-related domain / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / SANT/Myb domain / Helicase conserved C-terminal domain / Homeobox-like domain superfamily / Bromodomain, conserved site / Bromodomain signature. / Bromodomain / Bromodomain profile. / bromo domain / Bromodomain / Bromodomain-like superfamily / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / Winged helix-like DNA-binding domain superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Chromatin structure-remodeling complex protein RSC6 / Nuclear protein STH1/NPS1 / Chromatin structure-remodeling complex subunit RSC7 / Chromatin structure-remodeling complex protein RSC30 / Chromatin structure-remodeling complex protein RSC8 / Chromatin structure-remodeling complex subunit RSC4 / Chromatin structure-remodeling complex subunit RSC9 / Chromatin structure-remodeling complex subunit SFH1 / Chromatin structure-remodeling complex subunit RSC2 / Chromatin structure-remodeling complex protein RSC3 ...Chromatin structure-remodeling complex protein RSC6 / Nuclear protein STH1/NPS1 / Chromatin structure-remodeling complex subunit RSC7 / Chromatin structure-remodeling complex protein RSC30 / Chromatin structure-remodeling complex protein RSC8 / Chromatin structure-remodeling complex subunit RSC4 / Chromatin structure-remodeling complex subunit RSC9 / Chromatin structure-remodeling complex subunit SFH1 / Chromatin structure-remodeling complex subunit RSC2 / Chromatin structure-remodeling complex protein RSC3 / Chromatin structure-remodeling complex protein RSC58 / High temperature lethal protein 1
Similarity search - Component
Biological speciesSaccharomyces cerevisiae S288c (yeast) / Saccharomyces cerevisiae S288C (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.4 Å
AuthorsYe YP / Wu H
CitationJournal: Science / Year: 2019
Title: Structure of the RSC complex bound to the nucleosome.
Authors: Youpi Ye / Hao Wu / Kangjing Chen / Cedric R Clapier / Naveen Verma / Wenhao Zhang / Haiteng Deng / Bradley R Cairns / Ning Gao / Zhucheng Chen /
Abstract: The RSC complex remodels chromatin structure and regulates gene transcription. We used cryo-electron microscopy to determine the structure of yeast RSC bound to the nucleosome. RSC is delineated into ...The RSC complex remodels chromatin structure and regulates gene transcription. We used cryo-electron microscopy to determine the structure of yeast RSC bound to the nucleosome. RSC is delineated into the adenosine triphosphatase motor, the actin-related protein module, and the substrate recruitment module (SRM). RSC binds the nucleosome mainly through the motor, with the auxiliary subunit Sfh1 engaging the H2A-H2B acidic patch to enable nucleosome ejection. SRM is organized into three substrate-binding lobes poised to bind their respective nucleosomal epitopes. The relative orientations of the SRM and the motor on the nucleosome explain the directionality of DNA translocation and promoter nucleosome repositioning by RSC. Our findings shed light on RSC assembly and functionality, and they provide a framework to understand the mammalian homologs BAF/PBAF and the Sfh1 ortholog INI1/BAF47, which are frequently mutated in cancers.
History
DepositionMay 9, 2019-
Header (metadata) releaseNov 13, 2019-
Map releaseNov 13, 2019-
UpdateMar 27, 2024-
Current statusMar 27, 2024Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6k15
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_9905.png

Downloads & links

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Map

FileDownload / File: emd_9905.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationRSC SRM region
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 360 pix.
= 385.2 Å		1.07 Å/pix.
x 360 pix.
= 385.2 Å		1.07 Å/pix.
x 360 pix.
= 385.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.07 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.02 / Movie #1: 0.02
Minimum - Maximum-0.055208553 - 0.09196134
Average (Standard dev.)0.0001682169 (±0.0018277868)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 385.2 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.071.071.07
M x/y/z360360360
origin x/y/z0.0000.0000.000
length x/y/z385.200385.200385.200
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ300300300
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS360360360
D min/max/mean-0.0550.0920.000

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Supplemental data

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Additional map: RSC HB lobe

Fileemd_9905_additional_1.map
AnnotationRSC HB lobe
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: RSC DB lobe

Fileemd_9905_additional_2.map
AnnotationRSC DB lobe
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : RSC

EntireName: RSC
Components
  • Complex: RSC
    • Protein or peptide: Chromatin structure-remodeling complex subunit RSC7
    • Protein or peptide: Chromatin structure-remodeling complex protein RSC8
    • Protein or peptide: Chromatin structure-remodeling complex subunit RSC9
    • Protein or peptide: Chromatin structure-remodeling complex protein RSC6
    • Protein or peptide: Chromatin structure-remodeling complex subunit SFH1
    • Protein or peptide: Chromatin structure-remodeling complex protein RSC58
    • Protein or peptide: Nuclear protein STH1/NPS1
    • Protein or peptide: High temperature lethal protein 1
    • Protein or peptide: Chromatin structure-remodeling complex protein RSC30
    • Protein or peptide: Chromatin structure-remodeling complex protein RSC3
    • Protein or peptide: Chromatin structure-remodeling complex subunit RSC4
    • Protein or peptide: Chromatin structure-remodeling complex subunit RSC2
  • Ligand: ZINC ION

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Supramolecule #1: RSC

SupramoleculeName: RSC / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#12
Source (natural)Organism: Saccharomyces cerevisiae S288c (yeast)

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Macromolecule #1: Chromatin structure-remodeling complex subunit RSC7

MacromoleculeName: Chromatin structure-remodeling complex subunit RSC7 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: S288c
Molecular weightTheoretical: 49.71652 KDa
SequenceString: MSDSEGGLAS EVEHEKRSRS TSNRPNYAID TEDLDIDEND ENEDDDYREE EANEGVNEEE ISDEEEQINK SGRNKRRHVD EEEDLSEDK GVTRSRNRSK FKKPVFPGID DAEENLNPLK VVNEEYVLPD DPEGETKITA DGDLLGGREF LVRTFTLTEK G NRKFMLAT ...String:
MSDSEGGLAS EVEHEKRSRS TSNRPNYAID TEDLDIDEND ENEDDDYREE EANEGVNEEE ISDEEEQINK SGRNKRRHVD EEEDLSEDK GVTRSRNRSK FKKPVFPGID DAEENLNPLK VVNEEYVLPD DPEGETKITA DGDLLGGREF LVRTFTLTEK G NRKFMLAT EPARIVGFRD SYLFFQTHPN LYKFILNQTQ KNDLIDRGVL PYSYRNRQIA LVTARGVFKE FGAKIIRGGK HI TDDYYAS ELRTKGNVIE GKLAGDPIDK SARALETMMY PASENGINPA KNQVEFFEHR PHGHMSNSNI IASGSKLSST NWL YQHSAA CSRFNSDLFY DRVKVLLVDQ QGLRDAYTNI LHIPESTQST TVLGWRRSKN DSPSDTSIVY ETVIHDNDLN KPKT GLSEI PKEIYEDVVD EDVLRAITEQ QNFEKCNEYI

UniProtKB: Chromatin structure-remodeling complex subunit RSC7

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Macromolecule #2: Chromatin structure-remodeling complex protein RSC8

MacromoleculeName: Chromatin structure-remodeling complex protein RSC8 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: S288c
Molecular weightTheoretical: 63.253965 KDa
SequenceString: MSDTEKDKDV PMVDSHEATE EPPTTSTNTP SFPHLAQEQA KEESATLGAE VAHKKINYEQ EAQKLEEKAL RFLAKQTHPV IIPSFASWF DISKIHEIEK RSNPDFFNDS SRFKTPKAYK DTRNFIINTY RLSPYEYLTI TAVRRNVAMD VASIVKIHAF L EKWGLINY ...String:
MSDTEKDKDV PMVDSHEATE EPPTTSTNTP SFPHLAQEQA KEESATLGAE VAHKKINYEQ EAQKLEEKAL RFLAKQTHPV IIPSFASWF DISKIHEIEK RSNPDFFNDS SRFKTPKAYK DTRNFIINTY RLSPYEYLTI TAVRRNVAMD VASIVKIHAF L EKWGLINY QIDPRTKPSL IGPSFTGHFQ VVLDTPQGLK PFLPENVIKQ EVEGGDGAEP QVKKEFPVNL TIKKNVYDSA QD FNALQDE SRNSRQIHKV YICHTCGNES INVRYHNLRA RDTNLCSRCF QEGHFGANFQ SSDFIRLENN GNSVKKNWSD QEM LLLLEG IEMYEDQWEK IADHVGGHKR VEDCIEKFLS LPIEDNYIRE VVGSTLNGKG GDSRDGSVSG SKLMECVNDA VQTL LQGDD KLGKVSDKSR EISEKYIEES QAIIQELVKL TMEKLESKFT KLCDLETQLE MEKLKYVKES EKMLNDRLSL SKQIL DLNK SLEELNVSKK LVLISEQVDS GIQLVEKDQE GDDEDGNTAT GHGVKRVGKE GEEVGEGDSI AKLQPQVYKP WSL

UniProtKB: Chromatin structure-remodeling complex protein RSC8

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Macromolecule #3: Chromatin structure-remodeling complex subunit RSC9

MacromoleculeName: Chromatin structure-remodeling complex subunit RSC9 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: S288c
Molecular weightTheoretical: 65.289309 KDa
SequenceString: MNSLASNTPL NGTPVSEAPA TSSEPVNMFE TMVANPIKVS RLQSNGVLTG PAANTKSIHY SLANFNVFQS LPKETARGVD DLTRMEMAL LSGIPEEIKW SLKKYLTYSN KAPYMISLRT LPDLLPLFKT FILPLERIVE GLNKSSICDS KAMDSLQMGL N ALLILRNL ...String:
MNSLASNTPL NGTPVSEAPA TSSEPVNMFE TMVANPIKVS RLQSNGVLTG PAANTKSIHY SLANFNVFQS LPKETARGVD DLTRMEMAL LSGIPEEIKW SLKKYLTYSN KAPYMISLRT LPDLLPLFKT FILPLERIVE GLNKSSICDS KAMDSLQMGL N ALLILRNL AQDTDSVQIL VKDREIKSFI LFILKKFQCV ATGDNKWQLY EGNATFFNEL THYTLDLMEA ISSYIAPAMK DD HYFQTLV SILNYTKDRY MVISILRSLS RLLVRSKANE ESAADNLDHK TLSLIVSFLL LECDSELIIA SLDFLYQYIL PGS QRITEL FKSKECSLIL EATLPNLLSY NIATPDYHLL QKHKIRLIKR LKPPAPKEPP NLSEDLFQQL FKLNEPLRST AWLR CCFEP VQEAEFTQIS LWRSYESKFG QPVRESGRKL LPAVEFIKNV SNAFNNAAAI VITDPVTGKK RFVIKGIQPR FKALG IADG ERESQVPISA LKSKFLNDSK EITPARQNSI PEVKFPQELS DVSKVACTFL CLLSNDTDDG AGSAFCQRIR PLVLHK LAD IPPLTLALSE YMENTSGL

UniProtKB: Chromatin structure-remodeling complex subunit RSC9

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Macromolecule #4: Chromatin structure-remodeling complex protein RSC6

MacromoleculeName: Chromatin structure-remodeling complex protein RSC6 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: S288c
Molecular weightTheoretical: 54.222691 KDa
SequenceString: MVTQTNPVPV TYPTDAYIPT YLPDDKVSNL ADLKKLIEMD SRLDLYLTRR RLDTSINLPT NTKTKDHPPN KEMLRIYVYN TTESSPRSD SGTPADSGKT TWTLRIEGKL LHESANGKHP FSEFLEGVAV DFKRLKPLGM GKKRKRDSSL SLPLNLQQPE Y NDQDSTMG ...String:
MVTQTNPVPV TYPTDAYIPT YLPDDKVSNL ADLKKLIEMD SRLDLYLTRR RLDTSINLPT NTKTKDHPPN KEMLRIYVYN TTESSPRSD SGTPADSGKT TWTLRIEGKL LHESANGKHP FSEFLEGVAV DFKRLKPLGM GKKRKRDSSL SLPLNLQQPE Y NDQDSTMG DNDNGEDEDS AEAESREEIV DALEWNYDEN NVVEFDGIDI KRQGKDNLRC SITIQLRGVD GGKVQYSPNL AT LIGMQTG SVNDAVYSIY KYILINNLFV TEQTEAQDGS NDAEDSSNEN NNKNGAGDDD GVEGSTPKDK PELGEVKLDS LLQ KVLDTN AAHLPLMNVV QTVNKLVSPL PPIILDYTID LSKDTTYGAT TLDVDVSHIL HQPQPQPNLQ KEEETDAEDT AKLR EITKL ALQLNSSAQK YQFFHELSLH PRETLTHYLW SSKQNELVLQ GDQYFNEDAA RTSDIYSNNN NDRSLMGNIS LLYSQ GRL

UniProtKB: Chromatin structure-remodeling complex protein RSC6

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Macromolecule #5: Chromatin structure-remodeling complex subunit SFH1

MacromoleculeName: Chromatin structure-remodeling complex subunit SFH1 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: S288c
Molecular weightTheoretical: 48.83318 KDa
SequenceString: MSHQNQLIPQ AYISNFHNRL TNEDDGIPIF TMAQQTRQHK RAKVVNYAEY DNDLFDEFNM NGSNFNNADT HYKDNAVSHE NTPALTNGV TMDGSEYNVL ENMNGADSII SNNKYDAGSN MVVESLSGLN SNNNASNGPS NKAQAQDIGN AVLPDLQDQH H NPFNILRY ...String:
MSHQNQLIPQ AYISNFHNRL TNEDDGIPIF TMAQQTRQHK RAKVVNYAEY DNDLFDEFNM NGSNFNNADT HYKDNAVSHE NTPALTNGV TMDGSEYNVL ENMNGADSII SNNKYDAGSN MVVESLSGLN SNNNASNGPS NKAQAQDIGN AVLPDLQDQH H NPFNILRY PKIRDTFING KVVSPYRLNT DQETKANANS GEAIMIPITL DIEHMGHTIK DQFLWNYNDD SISPEEFASI YC KDLDMTS ATLQTQIANI IKEQLKDLEN IAATEIMSDL HVIINLTCNL QDRFFEDNFQ WNLNDKSLTP ERFATSIVQD LGL TREFIP LISQSLHETI LKIKKDWVDG HLIQDHVPND AAFGYLSGIR LDIDELGSNW CPRVEILTKE EIQKREIEKE RNLR RLKRE TDRLSRRGRR RLDDLETTMR M

UniProtKB: Chromatin structure-remodeling complex subunit SFH1

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Macromolecule #6: Chromatin structure-remodeling complex protein RSC58

MacromoleculeName: Chromatin structure-remodeling complex protein RSC58 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: S288c
Molecular weightTheoretical: 57.871309 KDa
SequenceString: MTESVGGNKL VDFLVNVQSI LNAASVKCHV VDESFPAKFF EKNPDKIYES YCKFIKNRSN SEGLIRNEDK LVLTTINKRF ENGEYEPIQ GGFYKLYHDI KLVCTILIHF YPQGTRNYQL VDKFYKFSSE LLLRECCRIG IALTQTNNIK SRSGKLLSGN E MDEYDDDD ...String:
MTESVGGNKL VDFLVNVQSI LNAASVKCHV VDESFPAKFF EKNPDKIYES YCKFIKNRSN SEGLIRNEDK LVLTTINKRF ENGEYEPIQ GGFYKLYHDI KLVCTILIHF YPQGTRNYQL VDKFYKFSSE LLLRECCRIG IALTQTNNIK SRSGKLLSGN E MDEYDDDD ATELDKIISY DFIKISMNYT VPISQTYQIR TKDMDLFSSI ISKSNLDKRP HELPNTNFKI NNVLPQTDIE NE APRLGFV GANTSNIPDP TLPPTEMMTR FLHPNWYALP TTVWLKYGNY NSWAPSFNEN GTVVDSTTRG LIWLERIGYM DLY EKNEKK VKQEELLNTN EEGINRKQND ENNKNVDGKS NGVQDDGGDN DNDATIASAN SESTENKEQF IIKLQNLYNW TPSN YIGDD EIENFRNGTP DKLVSDSLLK LKRLRKERIL NKVLKPTTEE RELYFKVKRI LKEVILAKKV SKVPINNVRA FPVLQ TNYN GSIPVVRAQP GRKRKHKK

UniProtKB: Chromatin structure-remodeling complex protein RSC58

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Macromolecule #7: Nuclear protein STH1/NPS1

MacromoleculeName: Nuclear protein STH1/NPS1 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: S288c
Molecular weightTheoretical: 156.982406 KDa
SequenceString: MLQEQSELMS TVMNNTPTTV AALAAVAAAS ETNGKLGSEE QPEITIPKPR SSAQLEQLLY RYRAIQNHPK ENKLEIKAIE DTFRNISRD QDIYETKLDT LRKSIDKGFQ YDEDLLNKHL VALQLLEKDT DVPDYFLDLP DTKNDNTTAI EVDYSEKKPI K ISADFNAK ...String:
MLQEQSELMS TVMNNTPTTV AALAAVAAAS ETNGKLGSEE QPEITIPKPR SSAQLEQLLY RYRAIQNHPK ENKLEIKAIE DTFRNISRD QDIYETKLDT LRKSIDKGFQ YDEDLLNKHL VALQLLEKDT DVPDYFLDLP DTKNDNTTAI EVDYSEKKPI K ISADFNAK AKSLGLESKF SNATKTALGD PDTEIRISAR ISNRINELER LPANLGTYSL DDCLEFITKD DLSSRMDTFK IK ALVELKS LKLLTKQKSI RQKLINNVAS QAHHNIPYLR DSPFTAAAQR SVQIRSKVIV PQTVRLAEEL ERQQLLEKRK KER NLHLQK INSIIDFIKE RQSEQWSRQE RCFQFGRLGA SLHNQMEKDE QKRIERTAKQ RLAALKSNDE EAYLKLLDQT KDTR ITQLL RQTNSFLDSL SEAVRAQQNE AKILHGEEVQ PITDEEREKT DYYEVAHRIK EKIDKQPSIL VGGTLKEYQL RGLEW MVSL YNNHLNGILA DEMGLGKTIQ SISLITYLYE VKKDIGPFLV IVPLSTITNW TLEFEKWAPS LNTIIYKGTP NQRHSL QHQ IRVGNFDVLL TTYEYIIKDK SLLSKHDWAH MIIDEGHRMK NAQSKLSFTI SHYYRTRNRL ILTGTPLQNN LPELWAL LN FVLPKIFNSA KTFEDWFNTP FANTGTQEKL ELTEEETLLI IRRLHKVLRP FLLRRLKKEV EKDLPDKVEK VIKCKLSG L QQQLYQQMLK HNALFVGAGT EGATKGGIKG LNNKIMQLRK ICNHPFVFDE VEGVVNPSRG NSDLLFRVAG KFELLDRVL PKFKASGHRV LMFFQMTQVM DIMEDFLRMK DLKYMRLDGS TKTEERTEML NAFNAPDSDY FCFLLSTRAG GLGLNLQTAD TVIIFDTDW NPHQDLQAQD RAHRIGQKNE VRILRLITTD SVEEVILERA MQKLDIDGKV IQAGKFDNKS TAEEQEAFLR R LIESETNR DDDDKAELDD DELNDTLARS ADEKILFDKI DKERMNQERA DAKAQGLRVP PPRLIQLDEL PKVFREDIEE HF KKEDSEP LGRIRQKKRV YYDDGLTEEQ FLEAVEDDNM SLEDAIKKRR EARERRRLRQ NGTKENEIET LENTPEASET SLI ENNSFT AAVDEETNAD KETTASRSKR RSSRKKRTIS IVTAEDKENT QEESTSQENG GAKVEEEVKS SSVEIINGSE SKKK KPKLT VKIKLNKTTV LENNDGKRAE EKPESKSPAK KTAAKKTKTK SKSLGIFPTV EKLVEEMREQ LDEVDSHPRT SIFEK LPSK RDYPDYFKVI EKPMAIDIIL KNCKNGTYKT LEEVRQALQT MFENARFYNE EGSWVYVDAD KLNEFTDEWF KEHSS

UniProtKB: Nuclear protein STH1/NPS1

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Macromolecule #8: High temperature lethal protein 1

MacromoleculeName: High temperature lethal protein 1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: S288c
Molecular weightTheoretical: 9.192524 KDa
SequenceString:
MSQNNTISSM NPERAYNNVT LKNLTAFQLL SQRENICELL NLVESTERHN SIINPERQRM SLEEMKKMLD ALKNERKK

UniProtKB: High temperature lethal protein 1

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Macromolecule #9: Chromatin structure-remodeling complex protein RSC30

MacromoleculeName: Chromatin structure-remodeling complex protein RSC30 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: S288c
Molecular weightTheoretical: 101.448211 KDa
SequenceString: MMDMQVRKVR KPPACTQCRK RKIGCDRAKP ICGNCVKYNK PDCFYPDGPG KMVAVPSASG MSTHGNGQGS NHFSQGNGVN QKNVMIQTQ YPIMQTSIEA FNFSFNPSVD TAMQWTKAAS YQNNNTNNNT APRQNSSTVS SNVHGNTIVR SDSPDVPSMD Q IREYNTRL ...String:
MMDMQVRKVR KPPACTQCRK RKIGCDRAKP ICGNCVKYNK PDCFYPDGPG KMVAVPSASG MSTHGNGQGS NHFSQGNGVN QKNVMIQTQ YPIMQTSIEA FNFSFNPSVD TAMQWTKAAS YQNNNTNNNT APRQNSSTVS SNVHGNTIVR SDSPDVPSMD Q IREYNTRL QLVNAQSFDY TDNPYSFNVG INQDSAVFDL MTSPFTQEEV LIKEIDFLKN KLLDLQSLQL KSLKEKSNLN AD NTTANKI NKTGENSKKG KVDGKRAGFD HQTSRTSQSS QKYFTALTIT DVQSLVQVKP LKDTPNYLFT KNFIIFRDHY LFK FYNILH DICHINQFKV SPPNNKNHQQ YMEVCKVNFP PKAIIIETLN SESLNNLNIE EFLPIFDKTL LLEFVHNSFP NGDT CPSFS TVDLPLSQLT KLGELTVLLL LLNDSMTLFN KQAINNHVSA LMNNLRLIRS QITLINLEYY DQETIKFIAI TKFYE SLYM HDDHKSSLDE DLSCLLSFQI KDFKLFHFLK KMYYSRHSLL GQSSFMVPAA ENLSPIPASI DTNDIPLIAN DLKLLE TQA KLINILQGVP FYLPVNLTKI ESLLETLTMG VSNTVDLYFH DNEVRKEWKD TLNFINTIVY TNFFLFVQNE SSLSMAV QH SSNNNKTSNS ERCAKDLMKI ISNMHIFYSI TFNFIFPIKS IKSFSSGNNR FHSNGKEFLF ANHFIEILQN FIAITFAI F QRCEVILYDE FYKNLSNEEI NVQLLLIHDK ILEILKKIEI IVSFLRDEMN SNGSFKSIKG FNKVLNLIKY MLRFSKKKQ NFARNSDNNN VTDYSQSAKN KNVLLKFPVS ELNRIYLKFK EISDFLMERE VVQRSIIIDK DLESDNLGIT TANFNDFYDA FYN

UniProtKB: Chromatin structure-remodeling complex protein RSC30

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Macromolecule #10: Chromatin structure-remodeling complex protein RSC3

MacromoleculeName: Chromatin structure-remodeling complex protein RSC3 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: S288c
Molecular weightTheoretical: 101.833961 KDa
SequenceString: MDIRGRKMKK PPACVQCRKR KIGCDRVKPI CGNCMKHNKM DCFYPDVPGQ YVPSSSSSSN TRQVANGPYL NSYYASRRVS KETAALLQK NPELASLEQI REYNTRLQLL NAQNQLNNRS SAANATLNQQ HTQYIPKSVP SLESKPVTSA NESSTPLNWV Q GPAIFHML ...String:
MDIRGRKMKK PPACVQCRKR KIGCDRVKPI CGNCMKHNKM DCFYPDVPGQ YVPSSSSSSN TRQVANGPYL NSYYASRRVS KETAALLQK NPELASLEQI REYNTRLQLL NAQNQLNNRS SAANATLNQQ HTQYIPKSVP SLESKPVTSA NESSTPLNWV Q GPAIFHML TSPYTQDEII NHEMNFLKGR LLELQEITGK KITGVNLDLK QDSSAQMQSS HSNRNQEEFL TIKKRKLSED GV TDGDGKP IPESERRPHL NEFKDLDPQF LDTNKVFNVF NSAISEEGRN RLWLLPKNIN KSSIFQIQYL IERDPFLFKF FND LNILIE TQFNGPLHDL VASRNSIERN SGISQILKFP SQSITQTLIN KYLSTITETN SILPILKPKR LLPIVEQLFP SNTI NKPNS KDFETIFQVF SVTNDQLLNL GFITLCLLIL FESLNSTVLI PLRDDEHLQL FNVLFNYLPL LKSNLTTLRF EIEKR SMCN IETLRFISLW KYYQFVMDTS SSSSFVIDYD EDMHMACLLS LNHETQNQSH ILTWNFIFKN YCWRHLFLGQ LPLLMS EPF TNSTPIIDPL LNNDFELIDF EVNLMKYLQS KDQQLSIDKI IQLIKLLKNK NIEVSQGCLT TPSIINNIMD SLIYRNS ML YLNFYLLLQF ETLKNYAKFN EILEDFLELS RETLFFVFSN LANIKFAGHE FTFINKSIVV LQTLVLMLLA LYQRSFDS S KRTNDANEIS EQTDIHSNND NSKRIKNKNV IHLIINKIAM LLSDYTKNCK KQNKLIENLI IKIKTISKYI KNLEENKVT TSADSNYSIN NGFSGISAEQ LIKLNHELSK ISESLIKTDF YEQRKNSTVS NGVLGAAAPV DSDANSDTFG LTKENFNEVF EAIRS

UniProtKB: Chromatin structure-remodeling complex protein RSC3

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Macromolecule #11: Chromatin structure-remodeling complex subunit RSC4

MacromoleculeName: Chromatin structure-remodeling complex subunit RSC4 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: S288c
Molecular weightTheoretical: 72.372375 KDa
SequenceString: MVVKKRKLAT EAGGSDERPK YLPGKHPKNQ EKTPHVDYNA PLNPKSELFL DDWHIPKFNR FISFTLDVLI DKYKDIFKDF IKLPSRKFH PQYYYKIQQP MSINEIKSRD YEYEDGPSNF LLDVELLTKN CQAYNEYDSL IVKNSMQVVM LIEFEVLKAK N LKRNYLIN ...String:
MVVKKRKLAT EAGGSDERPK YLPGKHPKNQ EKTPHVDYNA PLNPKSELFL DDWHIPKFNR FISFTLDVLI DKYKDIFKDF IKLPSRKFH PQYYYKIQQP MSINEIKSRD YEYEDGPSNF LLDVELLTKN CQAYNEYDSL IVKNSMQVVM LIEFEVLKAK N LKRNYLIN SEVKAKLLHY LNKLVDATEK KINQALLGAS SPKNLDDKVK LSEPFMELVD KDELPEYYEI VHSPMALSIV KQ NLEIGQY SKIYDFIIDM LLVFQNAHIF NDPSALIYKD ATTLTNYFNY LIQKEFFPEL QDLNERGEIN LEFDKFEFEN YLA IGGGGP AAAGALAISA LDNDIEPESN REDLIDQADY DFNHFEGLGN GYNRSLLTED YLLNPNNFKK LIAKPETVQS EVKN ERSTT SDIEKTNSLE SEHLKIPKYN VIKSMQKEMQ SLSEQHTMEY KPYKLIQQIY IFSSKNLYSQ ATKPLLGSRP SCNQN WVEY IFNGNELSQN ENAFSFMLQP MQTFLTLQSH LTSSLKDTET LLTINKEPVK SRTSNVNSNL SQPQQQENDV IGNDTK QDI ENLTIGGGNN NDIVGNDNDK RNNITEIFDI RLSEGLNHLM FRCEDKISHE TEFMNFWINV LP

UniProtKB: Chromatin structure-remodeling complex subunit RSC4

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Macromolecule #12: Chromatin structure-remodeling complex subunit RSC2

MacromoleculeName: Chromatin structure-remodeling complex subunit RSC2 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: S288c
Molecular weightTheoretical: 102.443664 KDa
SequenceString: MMPDDNSNSS TQNSSALYKD LRKEYESLFT LKEDSGLEIS PIFNVLPPKK DYPDYYAVIK NPVSFNTLKK RIPHYTDAQQ FMNDVVQIP WNAKTYNTRD SGIYKYALVL EKYLKDTIYP NLKEKYPQLV YPDLGPLPDE PGYEEFQQKL REKAEEVARA N AARAESSS ...String:
MMPDDNSNSS TQNSSALYKD LRKEYESLFT LKEDSGLEIS PIFNVLPPKK DYPDYYAVIK NPVSFNTLKK RIPHYTDAQQ FMNDVVQIP WNAKTYNTRD SGIYKYALVL EKYLKDTIYP NLKEKYPQLV YPDLGPLPDE PGYEEFQQKL REKAEEVARA N AARAESSS SMNSTEAARR LRKTRTSVKR ESEPGTDTNN DEDYEATDMD IDNPKDADFP DLIRKPLINI NPYTRKPLRD NR STTPSHS GTPQPLGPRH RQVSRTQVKR GRPPIIDLPY IQRMKNVMKV LKKEVLDSGI GLTDLFERLP DRHRDANYYI MIA NPISLQ DINKKVKTRR YKTFQEFQND FNLMLTNFRI SHRGDPESIK ISNILEKTFT SLARFELSKP DRSFIPEGEL RYPL DEVIV NNISYHVGDW ALLRNQNDPQ KPIVGQIFRL WKTPDGKQWL NACWYYRPEQ TVHRVDRLFY KNEVMKTGQY RDHLV SNLV GKCYVIHFTR YQRGNPDMKL EGPLFVCEFR YNESDKIFNK IRTWKACLPE EIRDLDEATI PVNGRKFFKY PSPIRH LLP ANATPHDRVP EPTMGSPDAP PLVGAVYMRP KMQRDDLGEY ATSDDCPRYI IRPNDSPEEG QVDIETGTIT TNTPTAN AL PKTGYSSSKL SSLRYNRSSM SLENQNAIGQ QQIPLSRVGS PGAGGPLTVQ GLKQHQLQRL QQQQHQYQQQ KRSQASRY N IPTIIDDLTS QASRGNLGNI MIDAASSFVL PISITKNVDV LQRTDLHSQT KRSGREEMFP WKKTKGEILW FRGPSVIVN ERIINSGDPH LSLPLNRWFT TNKKRKLEYE EVEETMEDVT GKDKDDDGLE PDVENEKESL PGPFVLGLRP SAKFTAHRLS MLRPPSSSS

UniProtKB: Chromatin structure-remodeling complex subunit RSC2

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Macromolecule #13: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 13 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statecell

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 2.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: DARK FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: EMDB MAP
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 280000
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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