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- EMDB-9763: Cryo-EM Structure of an Extracellular Contractile Injection Syste... -

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Basic information

Entry
Database: EMDB / ID: EMD-9763
TitleCryo-EM Structure of an Extracellular Contractile Injection System, PVC sheath/tube terminator in extended state
Map data
Sample
  • Complex: sheath/tube terminator and the last layer of tube in contracted state
    • Protein or peptide: Pvc16
    • Protein or peptide: Pvc1
Keywordsassembly / Photorhabdus asymbiotica / PVC / contractile injection system / bacteriophage-like / PROTEIN TRANSPORT
Function / homologyPvc16, N-terminal / Pvc16 N-terminal domain / Conserved hypothetical protein CHP02241 / Bacteriophage T4, Gp19, tail tube / T4-like virus tail tube protein gp19 / structural molecule activity / Uncharacterized protein / Conserved hypothetical phage tail region protein
Function and homology information
Biological speciesPhotorhabdus asymbiotica (bacteria) / Photorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 / 3105-77) (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsJiang F / Li N
CitationJournal: Cell / Year: 2019
Title: Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System.
Authors: Feng Jiang / Ningning Li / Xia Wang / Jiaxuan Cheng / Yaoguang Huang / Yun Yang / Jianguo Yang / Bin Cai / Yi-Ping Wang / Qi Jin / Ning Gao /
Abstract: Contractile injection systems (CISs) are cell-puncturing nanodevices that share ancestry with contractile tail bacteriophages. Photorhabdus virulence cassette (PVC) represents one group of ...Contractile injection systems (CISs) are cell-puncturing nanodevices that share ancestry with contractile tail bacteriophages. Photorhabdus virulence cassette (PVC) represents one group of extracellular CISs that are present in both bacteria and archaea. Here, we report the cryo-EM structure of an intact PVC from P. asymbiotica. This over 10-MDa device resembles a simplified T4 phage tail, containing a hexagonal baseplate complex with six fibers and a capped 117-nanometer sheath-tube trunk. One distinct feature of the PVC is the presence of three variants for both tube and sheath proteins, indicating a functional specialization of them during evolution. The terminal hexameric cap docks onto the topmost layer of the inner tube and locks the outer sheath in pre-contraction state with six stretching arms. Our results on the PVC provide a framework for understanding the general mechanism of widespread CISs and pave the way for using them as delivery tools in biological or therapeutic applications.
History
DepositionDec 24, 2018-
Header (metadata) releaseApr 10, 2019-
Map releaseApr 10, 2019-
UpdateMar 27, 2024-
Current statusMar 27, 2024Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.14
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 0.14
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6j0f
  • Surface level: 0.14
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6j0f
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_9763.png

Downloads & links

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Map

FileDownload / File: emd_9763.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.121 Å
Density
Contour LevelBy AUTHOR: 0.14 / Movie #1: 0.14
Minimum - Maximum-0.42477575 - 0.6404571
Average (Standard dev.)0.00210028 (±0.024169866)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 286.976 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.1211.1211.121
M x/y/z256256256
origin x/y/z0.0000.0000.000
length x/y/z286.976286.976286.976
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS256256256
D min/max/mean-0.4250.6400.002

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Supplemental data

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Sample components

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Entire : sheath/tube terminator and the last layer of tube in contracted state

EntireName: sheath/tube terminator and the last layer of tube in contracted state
Components
  • Complex: sheath/tube terminator and the last layer of tube in contracted state
    • Protein or peptide: Pvc16
    • Protein or peptide: Pvc1

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Supramolecule #1: sheath/tube terminator and the last layer of tube in contracted state

SupramoleculeName: sheath/tube terminator and the last layer of tube in contracted state
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Photorhabdus asymbiotica (bacteria)

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Macromolecule #1: Pvc16

MacromoleculeName: Pvc16 / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Photorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 / 3105-77) (bacteria)
Strain: ATCC 43949 / 3105-77
Molecular weightTheoretical: 32.373434 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MLNTQTIIDV NKAMDAMLRA YLNQDIAIRF DLPELDTMQS DAMVSIFLYD IHEDLQLRSA ESRGFDVYAG RLLPGWVNIK CNYLITYWE ASKPATDASS PDSQPDNQAI QVMSQVLNAL INNRQLAGIP GAYTQVVPPK ESLNSLGNFW QSLGNRPRLS L NYSVTVPV ...String:
MLNTQTIIDV NKAMDAMLRA YLNQDIAIRF DLPELDTMQS DAMVSIFLYD IHEDLQLRSA ESRGFDVYAG RLLPGWVNIK CNYLITYWE ASKPATDASS PDSQPDNQAI QVMSQVLNAL INNRQLAGIP GAYTQVVPPK ESLNSLGNFW QSLGNRPRLS L NYSVTVPV SLNDGQDSAT PVTAVSSTVE QTASLSQEVV SHALRELLIT ELGGGEDNRL VLSKVELSAV KETMTQDSPA QM IILLSVS GITRQEYLKE IDNIFDRWVN NAEVITTIDD CGIRIESITK DNLVGI

UniProtKB: Uncharacterized protein

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Macromolecule #2: Pvc1

MacromoleculeName: Pvc1 / type: protein_or_peptide / ID: 2 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Photorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 / 3105-77) (bacteria)
Strain: ATCC 43949 / 3105-77
Molecular weightTheoretical: 16.532557 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MSTSTSQIAV EYPIPVYRFI VSVGDEKIPF NSVSGLDISY DTIEYRDGVG NWFKMPGQSQ STNITLRKGV FPGKTELFDW INSIQLNQV EKKDITISLT NDAGTELLMT WNVSNAFPTS LTSPSFDATS NDIAVQEITL MADRVIMQAV

UniProtKB: Conserved hypothetical phage tail region protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statehelical array

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Detector mode: INTEGRATING / Average electron dose: 46.4 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: PROJECTION MATCHING
Final reconstructionApplied symmetry - Point group: C6 (6 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 45000

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