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TitleCryo-EM Structure and Assembly of an Extracellular Contractile Injection System.
Journal, issue, pagesCell, Vol. 177, Issue 2, Page 370-383.e15, Year 2019
Publish dateApr 4, 2019
AuthorsFeng Jiang / Ningning Li / Xia Wang / Jiaxuan Cheng / Yaoguang Huang / Yun Yang / Jianguo Yang / Bin Cai / Yi-Ping Wang / Qi Jin / Ning Gao /
PubMed AbstractContractile injection systems (CISs) are cell-puncturing nanodevices that share ancestry with contractile tail bacteriophages. Photorhabdus virulence cassette (PVC) represents one group of ...Contractile injection systems (CISs) are cell-puncturing nanodevices that share ancestry with contractile tail bacteriophages. Photorhabdus virulence cassette (PVC) represents one group of extracellular CISs that are present in both bacteria and archaea. Here, we report the cryo-EM structure of an intact PVC from P. asymbiotica. This over 10-MDa device resembles a simplified T4 phage tail, containing a hexagonal baseplate complex with six fibers and a capped 117-nanometer sheath-tube trunk. One distinct feature of the PVC is the presence of three variants for both tube and sheath proteins, indicating a functional specialization of them during evolution. The terminal hexameric cap docks onto the topmost layer of the inner tube and locks the outer sheath in pre-contraction state with six stretching arms. Our results on the PVC provide a framework for understanding the general mechanism of widespread CISs and pave the way for using them as delivery tools in biological or therapeutic applications.
External linksCell / PubMed:30905475
MethodsEM (helical sym.) / EM (single particle)
Resolution2.9 - 6.2 Å
Structure data

9760

6j0b

EMDB-9760, PDB-6j0b:
Cryo-EM Structure of an Extracellular Contractile Injection System, PVC sheath-tube complex in extended state
Method: EM (helical sym.) / Resolution: 2.9 Å

9761

6j0c

EMDB-9761, PDB-6j0c:
Cryo-EM Structure of an Extracellular Contractile Injection System, PVC sheath complex in contracted state
Method: EM (helical sym.) / Resolution: 3.7 Å

EMDB-9762:
Cryo-EM Structure of an Extracellular Contractile Injection System, full length PVC particle in extended state
Method: EM (single particle) / Resolution: 6.2 Å

9763

6j0f

EMDB-9763, PDB-6j0f:
Cryo-EM Structure of an Extracellular Contractile Injection System, PVC sheath/tube terminator in extended state
Method: EM (single particle) / Resolution: 3.8 Å

9764

6j0m

EMDB-9764, PDB-6j0m:
Cryo-EM Structure of an Extracellular Contractile Injection System, PVC baseplate in extended state (reconstructed with C3 symmetry)
Method: EM (single particle) / Resolution: 3.9 Å

9765

6j0n

EMDB-9765, PDB-6j0n:
Cryo-EM Structure of an Extracellular Contractile Injection System, baseplate in extended state, refined in C6 symmetry
Method: EM (single particle) / Resolution: 3.5 Å

Source
  • photorhabdus asymbiotica (bacteria)
  • photorhabdus asymbiotica subsp. asymbiotica (strain atcc 43949 / 3105-77) (bacteria)
KeywordsPROTEIN TRANSPORT / assembly / Photorhabdus asymbiotica / PVC / contractile injection system / bacteriophage-like

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