protein-RNA complex assembly / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / structural constituent of chromatin / nucleosome / nucleosome assembly / large ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding ...protein-RNA complex assembly / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / structural constituent of chromatin / nucleosome / nucleosome assembly / large ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / DNA binding / RNA binding / zinc ion binding / membrane 類似検索 - 分子機能
Ribosomal protein L14, KOW motif / Linker histone H1/H5 / Ribosomal L15/L27a, N-terminal / Ribosomal protein L28e / : / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site ...Ribosomal protein L14, KOW motif / Linker histone H1/H5 / Ribosomal L15/L27a, N-terminal / Ribosomal protein L28e / : / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L44e signature. / Ribosomal protein L19, eukaryotic / Ribosomal protein L13e / Ribosomal protein L13e / 60S ribosomal protein L18a/ L20, eukaryotes / : / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L30e signature 1. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / : / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein 60S L18 and 50S L18e / Ribosomal Protein L6, KOW domain / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L30e, conserved site / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / : / Ribosomal protein L6e / Ribosomal protein L34Ae / Ribosomal protein L34e / 60S ribosomal protein L19 / Ribosomal protein L30/YlxQ / Ribosomal protein L7A/L8 / 60S ribosomal protein L6E / 60S ribosomal protein L35 / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L18e / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L7, eukaryotic / Ribosomal protein L30, N-terminal / Ribosomal L30 N-terminal domain / Ribosomal protein L37ae / Ribosomal L37ae protein family / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal_L19e / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e, N-terminal domain / Ribosomal protein L14e domain / Ribosomal protein L14 / Ribosomal protein L35A / Ribosomal protein L35Ae / Ribosomal protein L39e / Ribosomal protein L39e domain superfamily / Ribosomal protein L35A superfamily / Ribosomal L39 protein / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L14 / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L1e signature. / Ribosomal protein L31e / Ribosomal protein L31e domain superfamily / Ribosomal protein L31e / Ribosomal_L31e / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein L21e / Ribosomal protein L21e signature. / Ribosomal protein L24e-related / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L24e / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein L37e, conserved site / Ribosomal protein L37e signature. / Ribosomal protein L37e / Ribosomal protein L37e / Ribosomal_L15e / Ribosomal protein L4, eukaryotic and archaeal type / Ribosomal protein L15e 類似検索 - ドメイン・相同性
Large ribosomal subunit protein eL28 / 60S ribosomal protein L11, putative / 60S ribosomal protein L29 / 60S ribosomal protein L4, putative / Ribosomal protein L15 / 60S ribosomal protein L5, putative / 60S ribosomal protein L44, putative / 60S ribosomal protein L32, putative / 60S ribosomal protein L37a, putative / 60S ribosomal protein L34, putative ...Large ribosomal subunit protein eL28 / 60S ribosomal protein L11, putative / 60S ribosomal protein L29 / 60S ribosomal protein L4, putative / Ribosomal protein L15 / 60S ribosomal protein L5, putative / 60S ribosomal protein L44, putative / 60S ribosomal protein L32, putative / 60S ribosomal protein L37a, putative / 60S ribosomal protein L34, putative / 60S ribosomal protein L17, putative / Ribosomal protein L21E (60S), putative / 60S ribosomal protein L26, putative / 60S ribosomal protein L30, putative / 60S ribosomal protein L6, putative / 60S ribosomal protein L7, putative / 60S ribosomal protein L2, putative / 40S ribosomal protein L14, putative / Ribosomal protein L38, putative / Ribosomal protein L39, putative / Ribosomal protein L37 / 60S ribosomal protein L18a / Large ribosomal subunit protein eL22 / Large ribosomal subunit protein eL18 / Ribosomal protein L24, putative / 60S ribosomal protein L35, putative / 60S ribosomal protein L23a, putative / Large ribosomal subunit protein uL15 / 60S ribosomal protein L23, putative / Ribosomal protein L35A, putative / 60S ribosomal protein L13 / 60S ribosomal protein L7a / Ribosomal protein L19-like protein / Ribosomal protein L27 / Ribosomal protein L13 / Ribosomal protein L36 / 60S ribosomal subunit protein L31 / 60S ribosomal protein L13a 類似検索 - 構成要素
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM29169
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
35GM118093
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM028301
米国
National Science Foundation (NSF, United States)
GRFP DGE-11-44155
米国
Howard Hughes Medical Institute (HHMI)
HHMI
米国
引用
ジャーナル: Proc Natl Acad Sci U S A / 年: 2016 タイトル: Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit. 著者: Zheng Liu / Cristina Gutierrez-Vargas / Jia Wei / Robert A Grassucci / Madhumitha Ramesh / Noel Espina / Ming Sun / Beril Tutuncuoglu / Susan Madison-Antenucci / John L Woolford / Liang Tong / Joachim Frank / 要旨: Ribosomes of trypanosomatids, a family of protozoan parasites causing debilitating human diseases, possess multiply fragmented rRNAs that together are analogous to 28S rRNA, unusually large rRNA ...Ribosomes of trypanosomatids, a family of protozoan parasites causing debilitating human diseases, possess multiply fragmented rRNAs that together are analogous to 28S rRNA, unusually large rRNA expansion segments, and r-protein variations compared with other eukaryotic ribosomes. To investigate the architecture of the trypanosomatid ribosomes, we determined the 2.5-Å structure of the Trypanosoma cruzi ribosome large subunit by single-particle cryo-EM. Examination of this structure and comparative analysis of the yeast ribosomal assembly pathway allowed us to develop a stepwise assembly model for the eight pieces of the large subunit rRNAs and a number of ancillary "glue" proteins. This model can be applied to the characterization of Trypanosoma brucei and Leishmania spp. ribosomes as well. Together with other details, our atomic-level structure may provide a foundation for structure-based design of antitrypanosome drugs.