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-Structure paper
Title | Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit. |
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Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 113, Issue 43, Page 12174-12179, Year 2016 |
Publish date | Oct 25, 2016 |
Authors | Zheng Liu / Cristina Gutierrez-Vargas / Jia Wei / Robert A Grassucci / Madhumitha Ramesh / Noel Espina / Ming Sun / Beril Tutuncuoglu / Susan Madison-Antenucci / John L Woolford / Liang Tong / Joachim Frank / |
PubMed Abstract | Ribosomes of trypanosomatids, a family of protozoan parasites causing debilitating human diseases, possess multiply fragmented rRNAs that together are analogous to 28S rRNA, unusually large rRNA ...Ribosomes of trypanosomatids, a family of protozoan parasites causing debilitating human diseases, possess multiply fragmented rRNAs that together are analogous to 28S rRNA, unusually large rRNA expansion segments, and r-protein variations compared with other eukaryotic ribosomes. To investigate the architecture of the trypanosomatid ribosomes, we determined the 2.5-Å structure of the Trypanosoma cruzi ribosome large subunit by single-particle cryo-EM. Examination of this structure and comparative analysis of the yeast ribosomal assembly pathway allowed us to develop a stepwise assembly model for the eight pieces of the large subunit rRNAs and a number of ancillary "glue" proteins. This model can be applied to the characterization of Trypanosoma brucei and Leishmania spp. ribosomes as well. Together with other details, our atomic-level structure may provide a foundation for structure-based design of antitrypanosome drugs. |
External links | Proc Natl Acad Sci U S A / PubMed:27791004 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.54 Å |
Structure data | |
Chemicals | ChemComp-MG: ChemComp-ZN: ChemComp-HOH: |
Source |
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Keywords | RIBOSOME / Trypanosoma cruzi / ribosomal subunit / assmebly / multiply fragmented rRNAs / 2.5 angstrom cryo-EM structure |