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- EMDB-7595: Promotion of Virus Assembly and Organization by the Measles Virus... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-7595 | |||||||||||||||||||||
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Title | Promotion of Virus Assembly and Organization by the Measles Virus Matrix Protein: RNP average (Class 1) | |||||||||||||||||||||
![]() | RNP average of measles virus, Class 1 | |||||||||||||||||||||
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Biological species | ![]() | |||||||||||||||||||||
Method | subtomogram averaging / cryo EM / Resolution: 35.0 Å | |||||||||||||||||||||
![]() | Ke Z / Strauss JD / Hampton CM / Dillard RS / Wright ER | |||||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Promotion of virus assembly and organization by the measles virus matrix protein. Authors: Zunlong Ke / Joshua D Strauss / Cheri M Hampton / Melinda A Brindley / Rebecca S Dillard / Fredrick Leon / Kristen M Lamb / Richard K Plemper / Elizabeth R Wright / ![]() Abstract: Measles virus (MeV) remains a major human pathogen, but there are presently no licensed antivirals to treat MeV or other paramyxoviruses. Here, we use cryo-electron tomography (cryo-ET) to elucidate ...Measles virus (MeV) remains a major human pathogen, but there are presently no licensed antivirals to treat MeV or other paramyxoviruses. Here, we use cryo-electron tomography (cryo-ET) to elucidate the principles governing paramyxovirus assembly in MeV-infected human cells. The three-dimensional (3D) arrangement of the MeV structural proteins including the surface glycoproteins (F and H), matrix protein (M), and the ribonucleoprotein complex (RNP) are characterized at stages of virus assembly and budding, and in released virus particles. The M protein is observed as an organized two-dimensional (2D) paracrystalline array associated with the membrane. A two-layered F-M lattice is revealed suggesting that interactions between F and M may coordinate processes essential for MeV assembly. The RNP complex remains associated with and in close proximity to the M lattice. In this model, the M lattice facilitates the well-ordered incorporation and concentration of the surface glycoproteins and the RNP at sites of virus assembly. | |||||||||||||||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 1.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14.9 KB 14.9 KB | Display Display | ![]() |
Images | ![]() | 26 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 79.1 KB | Display | ![]() |
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Full document | ![]() | 78.2 KB | Display | |
Data in XML | ![]() | 494 B | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7565C ![]() 7566C ![]() 7587C ![]() 7588C ![]() 7590C ![]() 7591C ![]() 7594C ![]() 7596C ![]() 7597C C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | RNP average of measles virus, Class 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 5.88 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Measles virus
Entire | Name: ![]() |
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Components |
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-Supramolecule #1: Measles virus
Supramolecule | Name: Measles virus / type: virus / ID: 1 / Parent: 0 / Details: Measles virus RNP average, global / NCBI-ID: 11234 / Sci species name: Measles virus / Sci species strain: Edmonston / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: Yes / Virus empty: No |
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-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
Aggregation state | cell |
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Sample preparation
Buffer | pH: 7.4 |
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Grid | Model: Quantifoil R2/1 / Material: GOLD / Mesh: 200 / Support film - Material: CARBON |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 85 % / Chamber temperature: 293 K / Instrument: GATAN CRYOPLUNGE 3 |
Details | measles virus infected cells |
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Electron microscopy
Microscope | JEOL 2200FS |
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Specialist optics | Energy filter - Name: In-column Omega Filter / Energy filter - Lower energy threshold: 0 eV / Energy filter - Upper energy threshold: 20 eV |
Image recording | Film or detector model: DIRECT ELECTRON DE-20 (5k x 3k) / Digitization - Dimensions - Width: 5080 pixel / Digitization - Dimensions - Height: 3768 pixel / Digitization - Frames/image: 1-8 / Average exposure time: 0.4 sec. / Average electron dose: 2.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 2.0 mm / Nominal defocus max: 8.0 µm / Nominal defocus min: 4.0 µm / Nominal magnification: 20000 |
Sample stage | Specimen holder model: GATAN 914 HIGH TILT LIQUID NITROGEN CRYO TRANSFER TOMOGRAPHY HOLDER Cooling holder cryogen: NITROGEN |