+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-7328 | |||||||||
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Title | CryoEM structure of mouse PCDH15-1EC-LHFPL5 complex | |||||||||
Map data | primary map | |||||||||
Sample |
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Function / homology | Function and homology information detection of mechanical stimulus involved in equilibrioception / detection of mechanical stimulus involved in sensory perception / stereocilium tip / inner ear receptor cell stereocilium organization / righting reflex / detection of mechanical stimulus involved in sensory perception of sound / stereocilium bundle / inner ear auditory receptor cell differentiation / stereocilium / non-motile cilium assembly ...detection of mechanical stimulus involved in equilibrioception / detection of mechanical stimulus involved in sensory perception / stereocilium tip / inner ear receptor cell stereocilium organization / righting reflex / detection of mechanical stimulus involved in sensory perception of sound / stereocilium bundle / inner ear auditory receptor cell differentiation / stereocilium / non-motile cilium assembly / adult walking behavior / auditory receptor cell stereocilium organization / startle response / homophilic cell adhesion via plasma membrane adhesion molecules / inner ear development / photoreceptor outer segment / monoatomic ion transport / visual perception / actin filament organization / locomotory behavior / morphogenesis of an epithelium / sensory perception of sound / response to calcium ion / multicellular organism growth / membrane => GO:0016020 / cell adhesion / apical plasma membrane / calcium ion binding / extracellular region / membrane / plasma membrane / cytoplasm Similarity search - Function | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.5 Å | |||||||||
Authors | Gouaux E / Elferich J / Ge J | |||||||||
Citation | Journal: Elife / Year: 2018 Title: Structure of mouse protocadherin 15 of the stereocilia tip link in complex with LHFPL5. Authors: Jingpeng Ge / Johannes Elferich / April Goehring / Huaying Zhao / Peter Schuck / Eric Gouaux / Abstract: Hearing and balance involve the transduction of mechanical stimuli into electrical signals by deflection of bundles of stereocilia linked together by protocadherin 15 (PCDH15) and cadherin 23 'tip ...Hearing and balance involve the transduction of mechanical stimuli into electrical signals by deflection of bundles of stereocilia linked together by protocadherin 15 (PCDH15) and cadherin 23 'tip links'. PCDH15 transduces tip link tension into opening of a mechano-electrical transduction (MET) ion channel. PCDH15 also interacts with LHFPL5, a candidate subunit of the MET channel. Here we illuminate the PCDH15-LHFPL5 structure, showing how the complex is composed of PCDH15 and LHFPL5 subunit pairs related by a 2-fold axis. The extracellular cadherin domains define a mobile tether coupled to a rigid, 2-fold symmetric 'collar' proximal to the membrane bilayer. LHFPL5 forms extensive interactions with the PCDH15 transmembrane helices and stabilizes the overall PCDH15-LHFPL5 assembly. Our studies illuminate the architecture of the PCDH15-LHFPL5 complex, localize mutations associated with deafness, and shed new light on how forces in the PCDH15 tether may be transduced into the stereocilia membrane. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_7328.map.gz | 95.6 MB | EMDB map data format | |
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Header (meta data) | emd-7328-v30.xml emd-7328.xml | 12 KB 12 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_7328_fsc.xml | 10.6 KB | Display | FSC data file |
Images | emd_7328.png | 110 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-7328 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-7328 | HTTPS FTP |
-Validation report
Summary document | emd_7328_validation.pdf.gz | 555.2 KB | Display | EMDB validaton report |
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Full document | emd_7328_full_validation.pdf.gz | 554.8 KB | Display | |
Data in XML | emd_7328_validation.xml.gz | 12.1 KB | Display | |
Data in CIF | emd_7328_validation.cif.gz | 15.9 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-7328 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-7328 | HTTPS FTP |
-Related structure data
Related structure data | 6c14MC 7327C 6c10C 6c13C C: citing same article (ref.) M: atomic model generated by this map |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_7328.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | primary map | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.04 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : membrane protein complex
Entire | Name: membrane protein complex |
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Components |
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-Supramolecule #1: membrane protein complex
Supramolecule | Name: membrane protein complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Mus musculus (house mouse) |
Recombinant expression | Organism: Homo sapiens (human) |
-Macromolecule #1: Protocadherin-15
Macromolecule | Name: Protocadherin-15 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 37.458129 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: GQYDDHPPVF QKKFYIGGVS EDARMFASVL RVKATDRDTG NYSAMAYRLI IPPIKEGKEG FVVETYTGLI KTAMLFHNMR RSYFKFQVI ATDDYGKGLS GKADVLVSVV NQLDMQVIVS NVPPTLVEKK IEDLTEILDR YVQEQIPGAK VVVESIGARR H GDAYSLED ...String: GQYDDHPPVF QKKFYIGGVS EDARMFASVL RVKATDRDTG NYSAMAYRLI IPPIKEGKEG FVVETYTGLI KTAMLFHNMR RSYFKFQVI ATDDYGKGLS GKADVLVSVV NQLDMQVIVS NVPPTLVEKK IEDLTEILDR YVQEQIPGAK VVVESIGARR H GDAYSLED YSKCDLTVYA IDPQTNRAID RNELFKFLDG KLLDINKDFQ PYYGEGGRIL EIRTPEAVTS IKKRGESLGY TE GALLALA FIIILCCIPA ILVVLVSYRQ FKVRQAECTK TARIQSAMPA AKPAAPVPAA PAPPPPPPPP PPGAHLYEEL GES AMHKYE TALFESRLVP R |
-Macromolecule #2: LHFPL tetraspan subfamily member 5 protein
Macromolecule | Name: LHFPL tetraspan subfamily member 5 protein / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 25.06732 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: GSGGRATMVK LLPAQEAAKI YHTNYVRNSR AVGVMWGTLT ICFSVLVMAL FIQPYWIGDS VSTPQAGYFG LFSYCVGNVL SSELICKGG PLDFSSIPSR AFKTAMFFVA LAMFLIIGSI ICFSLFFVCN TATVYKICAW MQLAAATGLM IGCLVYPDGW D SSEVRRMC ...String: GSGGRATMVK LLPAQEAAKI YHTNYVRNSR AVGVMWGTLT ICFSVLVMAL FIQPYWIGDS VSTPQAGYFG LFSYCVGNVL SSELICKGG PLDFSSIPSR AFKTAMFFVA LAMFLIIGSI ICFSLFFVCN TATVYKICAW MQLAAATGLM IGCLVYPDGW D SSEVRRMC GEQTGKYTLG HCTIRWAFML AILSIGDALI LSFLAFVLGY RQDKLLPDDY KADGNEEVFE |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 Component:
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Grid | Model: Quantifoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE | ||||||||||||
Vitrification | Cryogen name: ETHANE-PROPANE / Chamber humidity: 100 % / Chamber temperature: 285 K / Instrument: FEI VITROBOT MARK III |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 74.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |