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- EMDB-70732: Structure of the sweet receptor bound to sucralose in the compact... -

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Basic information

Entry
Database: EMDB / ID: EMD-70732
TitleStructure of the sweet receptor bound to sucralose in the compact state, transmembrane domain
Map data
Sample
  • Complex: Human TAS1R2 and mouse TAS1R3 sweet receptor bound to sucralose
    • Protein or peptide: Taste receptor type 1 member 2
    • Protein or peptide: Taste receptor type 1 member 3
KeywordsMembrane protein / GPCR / SIGNALING PROTEIN
Function / homology
Function and homology information


Class C/3 (Metabotropic glutamate/pheromone receptors) / Sensory perception of sweet, bitter, and umami (glutamate) taste / sweet taste receptor complex / detection of chemical stimulus involved in sensory perception of sweet taste / sweet taste receptor activity / taste receptor activity / sensory perception of umami taste / sensory perception of sweet taste / G alpha (i) signalling events / Class C/3 (Metabotropic glutamate/pheromone receptors) ...Class C/3 (Metabotropic glutamate/pheromone receptors) / Sensory perception of sweet, bitter, and umami (glutamate) taste / sweet taste receptor complex / detection of chemical stimulus involved in sensory perception of sweet taste / sweet taste receptor activity / taste receptor activity / sensory perception of umami taste / sensory perception of sweet taste / G alpha (i) signalling events / Class C/3 (Metabotropic glutamate/pheromone receptors) / positive regulation of cytokinesis / G protein-coupled receptor activity / Sensory perception of sweet, bitter, and umami (glutamate) taste / G alpha (i) signalling events / receptor complex / G protein-coupled receptor signaling pathway / Golgi apparatus / membrane / plasma membrane
Similarity search - Function
GPCR, family 3, extracellular calcium-sensing receptor-related / G-protein coupled receptors family 3 signature 2. / GPCR, family 3, nine cysteines domain / GPCR, family 3, nine cysteines domain superfamily / Nine Cysteines Domain of family 3 GPCR / GPCR, family 3, conserved site / GPCR, family 3 / G-protein coupled receptors family 3 profile. / GPCR family 3, C-terminal / 7 transmembrane sweet-taste receptor of 3 GCPR ...GPCR, family 3, extracellular calcium-sensing receptor-related / G-protein coupled receptors family 3 signature 2. / GPCR, family 3, nine cysteines domain / GPCR, family 3, nine cysteines domain superfamily / Nine Cysteines Domain of family 3 GPCR / GPCR, family 3, conserved site / GPCR, family 3 / G-protein coupled receptors family 3 profile. / GPCR family 3, C-terminal / 7 transmembrane sweet-taste receptor of 3 GCPR / Receptor, ligand binding region / Receptor family ligand binding region / Periplasmic binding protein-like I
Similarity search - Domain/homology
Taste receptor type 1 member 2 / Taste receptor type 1 member 3
Similarity search - Component
Biological speciesHomo sapiens (human) / Mus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.67 Å
AuthorsWang H / Chen X / Dai Y / Lee CH
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM143282 United States
CitationJournal: Cell Res / Year: 2025
Title: Structure and activation mechanism of human sweet taste receptor.
Authors: Haolan Wang / Xiao Chen / Yaxin Dai / Shabareesh Pidathala / Yiming Niu / Chen Zhao / Siyu Li / Liang Wang / Chia-Hsueh Lee /
History
DepositionMay 20, 2025-
Header (metadata) releaseAug 27, 2025-
Map releaseAug 27, 2025-
UpdateNov 19, 2025-
Current statusNov 19, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_70732.png

Downloads & links

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Map

FileDownload / File: emd_70732.map.gz / Format: CCP4 / Size: 28.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.59 Å/pix.
x 196 pix.
= 311.522 Å		1.59 Å/pix.
x 196 pix.
= 311.522 Å		1.59 Å/pix.
x 196 pix.
= 311.522 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.5894 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.817
Minimum - Maximum-3.6424954 - 5.384679
Average (Standard dev.)-0.0044867992 (±0.0888737)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions196196196
Spacing196196196
CellA=B=C: 311.5224 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_70732_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_70732_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Human TAS1R2 and mouse TAS1R3 sweet receptor bound to sucralose

EntireName: Human TAS1R2 and mouse TAS1R3 sweet receptor bound to sucralose
Components
  • Complex: Human TAS1R2 and mouse TAS1R3 sweet receptor bound to sucralose
    • Protein or peptide: Taste receptor type 1 member 2
    • Protein or peptide: Taste receptor type 1 member 3

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Supramolecule #1: Human TAS1R2 and mouse TAS1R3 sweet receptor bound to sucralose

SupramoleculeName: Human TAS1R2 and mouse TAS1R3 sweet receptor bound to sucralose
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Taste receptor type 1 member 2

MacromoleculeName: Taste receptor type 1 member 2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 31.102051 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: RQLVFLEWHE APTIAVALLA ALGFLSTLAI LVIFWRHFQT PIVRSAGGPM CFLMLTLLLV AYMVVPVYVG PPKVSTCLCR QALFPLCFT ICISCIAVRS FQIVCAFKMA SRFPRAYSYW VRYQGPYVSM AFITVLKMVI VVIGMLATGL SPTTRTDPDD P KITIVSCN ...String:
RQLVFLEWHE APTIAVALLA ALGFLSTLAI LVIFWRHFQT PIVRSAGGPM CFLMLTLLLV AYMVVPVYVG PPKVSTCLCR QALFPLCFT ICISCIAVRS FQIVCAFKMA SRFPRAYSYW VRYQGPYVSM AFITVLKMVI VVIGMLATGL SPTTRTDPDD P KITIVSCN PNYRNSLLFN TSLDLLLSVV GFSFAYMGKE LPTNYNEAKF ITLSMTFYFT SSVSLCTFMS AYSGVLVTIV DL LVTVLNL LAISLGYFGP KCYMILFYPE RNTPAYFNSM I

UniProtKB: Taste receptor type 1 member 2

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Macromolecule #2: Taste receptor type 1 member 3

MacromoleculeName: Taste receptor type 1 member 3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 30.411141 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: PRRPKFLAWG EPVVLSLLLL LCLVLGLALA ALGLSVHHWD SPLVQASGGS QFCFGLICLG LFCLSVLLFP GRPSSASCLA QQPMAHLPL TGCLSTLFLQ AAETFVESEL PLSWANWLCS YLRGLWAWLV VLLATFVEAA LCAWYLIAFP PEVVTDWSVL P TEVLEHCH ...String:
PRRPKFLAWG EPVVLSLLLL LCLVLGLALA ALGLSVHHWD SPLVQASGGS QFCFGLICLG LFCLSVLLFP GRPSSASCLA QQPMAHLPL TGCLSTLFLQ AAETFVESEL PLSWANWLCS YLRGLWAWLV VLLATFVEAA LCAWYLIAFP PEVVTDWSVL P TEVLEHCH VRSWVSLGLV HITNAMLAFL CFLGTFLVQS QPGRYNRARG LTFAMLAYFI TWVSFVPLLA NVQVAYQPAV QM GAILVCA LGILVTFHLP KCYVLLWLPK LNTQEFFL

UniProtKB: Taste receptor type 1 member 3

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 61.6 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.1 µm / Nominal defocus min: 1.1 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.67 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 168365
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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