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- EMDB-70731: Structure of the sweet receptor bound to sucralose in the compact... -

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Basic information

Entry
Database: EMDB / ID: EMD-70731
TitleStructure of the sweet receptor bound to sucralose in the compact state, extracellular domain
Map data
Sample
  • Complex: Human TAS1R2 and mouse TAS1R3 sweet receptor bound to sucralose
    • Protein or peptide: Taste receptor type 1 member 2
    • Protein or peptide: Taste receptor type 1 member 3
KeywordsMembrane protein / GPCR / SIGNALING PROTEIN
Function / homology
Function and homology information


Class C/3 (Metabotropic glutamate/pheromone receptors) / Sensory perception of sweet, bitter, and umami (glutamate) taste / sweet taste receptor complex / detection of chemical stimulus involved in sensory perception of sweet taste / sweet taste receptor activity / taste receptor activity / sensory perception of umami taste / G alpha (i) signalling events / sensory perception of sweet taste / Class C/3 (Metabotropic glutamate/pheromone receptors) ...Class C/3 (Metabotropic glutamate/pheromone receptors) / Sensory perception of sweet, bitter, and umami (glutamate) taste / sweet taste receptor complex / detection of chemical stimulus involved in sensory perception of sweet taste / sweet taste receptor activity / taste receptor activity / sensory perception of umami taste / G alpha (i) signalling events / sensory perception of sweet taste / Class C/3 (Metabotropic glutamate/pheromone receptors) / positive regulation of cytokinesis / G protein-coupled receptor activity / Sensory perception of sweet, bitter, and umami (glutamate) taste / G alpha (i) signalling events / receptor complex / G protein-coupled receptor signaling pathway / Golgi apparatus / membrane / plasma membrane
Similarity search - Function
GPCR, family 3, extracellular calcium-sensing receptor-related / G-protein coupled receptors family 3 signature 2. / GPCR, family 3, nine cysteines domain / GPCR, family 3, nine cysteines domain superfamily / Nine Cysteines Domain of family 3 GPCR / GPCR, family 3, conserved site / GPCR, family 3 / G-protein coupled receptors family 3 profile. / GPCR family 3, C-terminal / 7 transmembrane sweet-taste receptor of 3 GCPR ...GPCR, family 3, extracellular calcium-sensing receptor-related / G-protein coupled receptors family 3 signature 2. / GPCR, family 3, nine cysteines domain / GPCR, family 3, nine cysteines domain superfamily / Nine Cysteines Domain of family 3 GPCR / GPCR, family 3, conserved site / GPCR, family 3 / G-protein coupled receptors family 3 profile. / GPCR family 3, C-terminal / 7 transmembrane sweet-taste receptor of 3 GCPR / Receptor, ligand binding region / Receptor family ligand binding region / Periplasmic binding protein-like I
Similarity search - Domain/homology
Taste receptor type 1 member 2 / Taste receptor type 1 member 3
Similarity search - Component
Biological speciesHomo sapiens (human) / Mus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.16 Å
AuthorsWang H / Chen X / Dai Y / Lee CH
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM143282 United States
CitationJournal: To Be Published
Title: Structure of the sweet receptor bound to sucralose in the compact state, extracellular domain
Authors: Wang H / Chen X / Dai Y / Lee CH
History
DepositionMay 20, 2025-
Header (metadata) releaseAug 27, 2025-
Map releaseAug 27, 2025-
UpdateAug 27, 2025-
Current statusAug 27, 2025Processing site: RCSB / Status: Released

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Structure visualization

Downloads & links

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Map

FileDownload / File: emd_70731.map.gz / Format: CCP4 / Size: 52.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.298 Å
Density
Contour LevelBy AUTHOR: 0.889
Minimum - Maximum-4.355765 - 5.9298244
Average (Standard dev.)-0.003252823 (±0.10109778)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions240240240
Spacing240240240
CellA=B=C: 311.52 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Human TAS1R2 and mouse TAS1R3 sweet receptor bound to sucralose

EntireName: Human TAS1R2 and mouse TAS1R3 sweet receptor bound to sucralose
Components
  • Complex: Human TAS1R2 and mouse TAS1R3 sweet receptor bound to sucralose
    • Protein or peptide: Taste receptor type 1 member 2
    • Protein or peptide: Taste receptor type 1 member 3

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Supramolecule #1: Human TAS1R2 and mouse TAS1R3 sweet receptor bound to sucralose

SupramoleculeName: Human TAS1R2 and mouse TAS1R3 sweet receptor bound to sucralose
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Taste receptor type 1 member 2

MacromoleculeName: Taste receptor type 1 member 2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 60.718883 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: SDFYLPGDYL LGGLFSLHAN MKGIVHLNFL QVPMCKEYEV KVIGYNLMQA MRFAVEEINN DSSLLPGVLL GYEIVDVCYI SNNVQPVLY FLAHEDNLLP IQEDYSNYSS RVVAVIGPDN SESVMTVANF LSLFLLPQIT YSAISDELRD KVRFPALLRT T PSADHHIE ...String:
SDFYLPGDYL LGGLFSLHAN MKGIVHLNFL QVPMCKEYEV KVIGYNLMQA MRFAVEEINN DSSLLPGVLL GYEIVDVCYI SNNVQPVLY FLAHEDNLLP IQEDYSNYSS RVVAVIGPDN SESVMTVANF LSLFLLPQIT YSAISDELRD KVRFPALLRT T PSADHHIE AMVQLMLHFR WNWIIVLVSN DTYGRDNGQL LGERVARRGI CIAFQETLPT LQPNQNMTSE ERQRLVTIVD KL QQSTARV VVVFSPDLTL YHFFNEVLRQ NFTGAVWIAS ESWAIDPVLH NLTELRHLGT FLGITIQSVP IPGFSEFREW GPQ AGPPPL SRTSQSYTCN QECDNCLNAT LSFNTILRLS GERVVYSVYS AVYAVAHALH SLLGCDKSTC TKRVVYPWQL LEEI WKVNF TLLDHQIFFD PQGDVALHLE IVQWQWDRSQ NPFQSVASYY PLQRQLKNIQ DISWHTINNT IPMSMCSKRC QSGQK KKPV GIHVCCFECI DCLPGTFLNH TEDEYECQAC PNNEWSYQSE TSCFKRQ

UniProtKB: Taste receptor type 1 member 2

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Macromolecule #2: Taste receptor type 1 member 3

MacromoleculeName: Taste receptor type 1 member 3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 59.94002 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: LCLSQQFKAQ GDYILGGLFP LGSTEEATLN QRTQPNSIPC NRFSPLGLFL AMAMKMAVEE INNGSALLPG LRLGYDLFDT CSEPVVTMK SSLMFLAKVG SQSIAAYCNY TQYQPRVLAV IGPHSSELAL ITGKFFSFFL MPQVSYSASM DRLSDRETFP S FFRTVPSD ...String:
LCLSQQFKAQ GDYILGGLFP LGSTEEATLN QRTQPNSIPC NRFSPLGLFL AMAMKMAVEE INNGSALLPG LRLGYDLFDT CSEPVVTMK SSLMFLAKVG SQSIAAYCNY TQYQPRVLAV IGPHSSELAL ITGKFFSFFL MPQVSYSASM DRLSDRETFP S FFRTVPSD RVQLQAVVTL LQNFSWNWVA ALGSDDDYGR EGLSIFSSLA NARGICIAHE GLVPQHDTSG QQLGKVLDVL RQ VNQSKVQ VVVLFASARA VYSLFSYSIH HGLSPKVWVA SESWLTSDLV MTLPNIARVG TVLGFLQRGA LLPEFSHYVE THL ALAADP AFCASLNAEL DLEEHVMGQR CPRCDDIMLQ NLSSGLLQNL SAGQLHHQIF ATYAAVYSVA QALHNTLQCN VSHC HVSEH VLPWQLLENM YNMSFHARDL TLQFDAEGNV DMEYDLKMWV WQSPTPVLHT VGTFNGTLQL QQSKMYWPGN QVPVS QCSR QCKDGQVRRV KGFHSCCYDC VDCKAGSYRK HPDDFTCTPC NQDQWSPEKS TACLP

UniProtKB: Taste receptor type 1 member 3

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 61.6 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.1 µm / Nominal defocus min: 1.1 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.16 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 168149
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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