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- EMDB-70730: Structure of the sweet receptor in the apo state -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-70730
TitleStructure of the sweet receptor in the apo state
Map dataStructure of the sweet receptor in the apo state
Sample
  • Complex: Human TAS1R2 and mouse TAS1R3 sweet receptor in the apo state
    • Protein or peptide: Taste receptor type 1 member 2
    • Protein or peptide: Taste receptor type 1 member 3
KeywordsMembrane protein / GPCR / apo state / SIGNALING PROTEIN
Function / homology
Function and homology information


Class C/3 (Metabotropic glutamate/pheromone receptors) / Sensory perception of sweet, bitter, and umami (glutamate) taste / sweet taste receptor complex / detection of chemical stimulus involved in sensory perception of sweet taste / sweet taste receptor activity / taste receptor activity / sensory perception of umami taste / G alpha (i) signalling events / sensory perception of sweet taste / Class C/3 (Metabotropic glutamate/pheromone receptors) ...Class C/3 (Metabotropic glutamate/pheromone receptors) / Sensory perception of sweet, bitter, and umami (glutamate) taste / sweet taste receptor complex / detection of chemical stimulus involved in sensory perception of sweet taste / sweet taste receptor activity / taste receptor activity / sensory perception of umami taste / G alpha (i) signalling events / sensory perception of sweet taste / Class C/3 (Metabotropic glutamate/pheromone receptors) / positive regulation of cytokinesis / G protein-coupled receptor activity / Sensory perception of sweet, bitter, and umami (glutamate) taste / G alpha (i) signalling events / receptor complex / G protein-coupled receptor signaling pathway / Golgi apparatus / membrane / plasma membrane
Similarity search - Function
GPCR, family 3, extracellular calcium-sensing receptor-related / G-protein coupled receptors family 3 signature 2. / GPCR, family 3, nine cysteines domain / GPCR, family 3, nine cysteines domain superfamily / Nine Cysteines Domain of family 3 GPCR / GPCR, family 3, conserved site / GPCR, family 3 / G-protein coupled receptors family 3 profile. / GPCR family 3, C-terminal / 7 transmembrane sweet-taste receptor of 3 GCPR ...GPCR, family 3, extracellular calcium-sensing receptor-related / G-protein coupled receptors family 3 signature 2. / GPCR, family 3, nine cysteines domain / GPCR, family 3, nine cysteines domain superfamily / Nine Cysteines Domain of family 3 GPCR / GPCR, family 3, conserved site / GPCR, family 3 / G-protein coupled receptors family 3 profile. / GPCR family 3, C-terminal / 7 transmembrane sweet-taste receptor of 3 GCPR / Receptor, ligand binding region / Receptor family ligand binding region / Periplasmic binding protein-like I
Similarity search - Domain/homology
Taste receptor type 1 member 2 / Taste receptor type 1 member 3
Similarity search - Component
Biological speciesHomo sapiens (human) / Mus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.51 Å
AuthorsWang H / Chen X / Dai Y / Lee CH
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM143282 United States
CitationJournal: To Be Published
Title: Structure of the sweet receptor in the apo state
Authors: Wang H / Chen X / Dai Y / Lee CH
History
DepositionMay 20, 2025-
Header (metadata) releaseSep 3, 2025-
Map releaseSep 3, 2025-
UpdateSep 3, 2025-
Current statusSep 3, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_70730.png

Downloads & links

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Map

FileDownload / File: emd_70730.map.gz / Format: CCP4 / Size: 52.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationStructure of the sweet receptor in the apo state
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.3 Å/pix.
x 240 pix.
= 311.52 Å		1.3 Å/pix.
x 240 pix.
= 311.52 Å		1.3 Å/pix.
x 240 pix.
= 311.52 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.298 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.584
Minimum - Maximum-2.6458194 - 4.6018276
Average (Standard dev.)0.0056927633 (±0.091683045)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions240240240
Spacing240240240
CellA=B=C: 311.52 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Half Map B

Fileemd_70730_half_map_1.map
AnnotationHalf Map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half Map A

Fileemd_70730_half_map_2.map
AnnotationHalf Map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Human TAS1R2 and mouse TAS1R3 sweet receptor in the apo state

EntireName: Human TAS1R2 and mouse TAS1R3 sweet receptor in the apo state
Components
  • Complex: Human TAS1R2 and mouse TAS1R3 sweet receptor in the apo state
    • Protein or peptide: Taste receptor type 1 member 2
    • Protein or peptide: Taste receptor type 1 member 3

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Supramolecule #1: Human TAS1R2 and mouse TAS1R3 sweet receptor in the apo state

SupramoleculeName: Human TAS1R2 and mouse TAS1R3 sweet receptor in the apo state
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Taste receptor type 1 member 2

MacromoleculeName: Taste receptor type 1 member 2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 93.126281 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: EPAENSDFYL PGDYLLGGLF SLHANMKGIV HLNFLQVPMC KEYEVKVIGY NLMQAMRFAV EEINNDSSLL PGVLLGYEIV DVCYISNNV QPVLYFLAHE DNLLPIQEDY SNYSSRVVAV IGPDNSESVM TVANFLSLFL LPQITYSAIS DELRDKVRFP A LLRTTPSA ...String:
EPAENSDFYL PGDYLLGGLF SLHANMKGIV HLNFLQVPMC KEYEVKVIGY NLMQAMRFAV EEINNDSSLL PGVLLGYEIV DVCYISNNV QPVLYFLAHE DNLLPIQEDY SNYSSRVVAV IGPDNSESVM TVANFLSLFL LPQITYSAIS DELRDKVRFP A LLRTTPSA DHHIEAMVQL MLHFRWNWII VLVSNDTYGR DNGQLLGERV ARRDICIAFQ ETLPTLQPNQ NMTSEERQRL VT IVDKLQQ STARVVVVFS PDLTLYHFFN EVLRQNFTGA VWIASESWAI DPVLHNLTEL RHLGTFLGIT IQSVPIPGFS EFR EWGPQA GPPPLSRTSQ SYTCNQECDN CLNATLSFNT ILRLSGERVV YSVYSAVYAV AHALHSLLGC DKSTCTKRVV YPWQ LLEEI WKVNFTLLDH QIFFDPQGDV ALHLEIVQWQ WDRSQNPFQS VASYYPLQRQ LKNIQDISWH TINNTIPMSM CSKRC QSGQ KKKPVGIHVC CFECIDCLPG TFLNHTEDEY ECQACPNNEW SYQSETSCFK RQLVFLEWHE APTIAVALLA ALGFLS TLA ILVIFWRHFQ TPIVRSAGGP MCFLMLTLLL VAYMVVPVYV GPPKVSTCLC RQALFPLCFT ICISCIAVRS FQIVCAF KM ASRFPRAYSY WVRYQGPYVS MAFITVLKMV IVVIGMLATG LSPTTRTDPD DPKITIVSCN PNYRNSLLFN TSLDLLLS V VGFSFAYMGK ELPTNYNEAK FITLSMTFYF TSSVSLCTFM SAYSGVLVTI VDLLVTVLNL LAISLGYFGP KCYMILFYP ERNTPAYFNS MIQGYTMRRD

UniProtKB: Taste receptor type 1 member 2

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Macromolecule #2: Taste receptor type 1 member 3

MacromoleculeName: Taste receptor type 1 member 3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 90.236078 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: LCLSQQFKAQ GDYILGGLFP LGSTEEATLN QRTQPNSIPC NRFSPLGLFL AMAMKMAVEE INNGSALLPG LRLGYDLFDT CSEPVVTMK SSLMFLAKVG SQSIAAYCNY TQYQPRVLAV IGPHSSELAL ITGKFFSFFL MPQVSYSASM DRLSDRETFP S FFRTVPSD ...String:
LCLSQQFKAQ GDYILGGLFP LGSTEEATLN QRTQPNSIPC NRFSPLGLFL AMAMKMAVEE INNGSALLPG LRLGYDLFDT CSEPVVTMK SSLMFLAKVG SQSIAAYCNY TQYQPRVLAV IGPHSSELAL ITGKFFSFFL MPQVSYSASM DRLSDRETFP S FFRTVPSD RVQLQAVVTL LQNFSWNWVA ALGSDDDYGR EGLSIFSSLA NARGICIAHE GLVPQHDTSG QQLGKVLDVL RQ VNQSKVQ VVVLFASARA VYSLFSYSIH HGLSPKVWVA SESWLTSDLV MTLPNIARVG TVLGFLQRGA LLPEFSHYVE THL ALAADP AFCASLNAEL DLEEHVMGQR CPRCDDIMLQ NLSSGLLQNL SAGQLHHQIF ATYAAVYSVA QALHNTLQCN VSHC HVSEH VLPWQLLENM YNMSFHARDL TLQFDAEGNV DMEYDLKMWV WQSPTPVLHT VGTFNGTLQL QQSKMYWPGN QVPVS QCSR QCKDGQVRRV KGFHSCCYDC VDCKAGSYRK HPDDFTCTPC NQDQWSPEKS TACLPRRPKF LAWGEPVVLS LLLLLC LVL GLALAALGLS VHHWDSPLVQ ASGGSQFCFG LICLGLFCLS VLLFPGRPSS ASCLAQQPMA HLPLTGCLST LFLQAAE TF VESELPLSWA NWLCSYLRGL WAWLVVLLAT FVEAALCAWY LIAFPPEVVT DWSVLPTEVL EHCHVRSWVS LGLVHITN A MLAFLCFLGT FLVQSQPGRY NRARGLTFAM LAYFITWVSF VPLLANVQVA YQPAVQMGAI LVCALGILVT FHLPKCYVL LWLPKLNTQE FFL

UniProtKB: Taste receptor type 1 member 3

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.6 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.1 µm / Nominal defocus min: 1.1 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.51 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 138568
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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