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- EMDB-70740: Structure of the human sweet receptor bound to advantame in the i... -

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Basic information

Entry
Database: EMDB / ID: EMD-70740
TitleStructure of the human sweet receptor bound to advantame in the intermediate state, extracellular domain
Map data
Sample
  • Complex: Human TAS1R2 and TAS1R3 sweet receptor bound to advantame
    • Protein or peptide: Taste receptor type 1 member 2
    • Protein or peptide: Taste receptor type 1 member 3
  • Ligand: advantame
KeywordsMembrane protein / GPCR / SIGNALING PROTEIN
Function / homology
Function and homology information


sweet taste receptor complex / detection of chemical stimulus involved in sensory perception of sweet taste / sweet taste receptor activity / taste receptor activity / sensory perception of umami taste / sensory perception of sweet taste / Class C/3 (Metabotropic glutamate/pheromone receptors) / positive regulation of cytokinesis / G protein-coupled receptor activity / Sensory perception of sweet, bitter, and umami (glutamate) taste ...sweet taste receptor complex / detection of chemical stimulus involved in sensory perception of sweet taste / sweet taste receptor activity / taste receptor activity / sensory perception of umami taste / sensory perception of sweet taste / Class C/3 (Metabotropic glutamate/pheromone receptors) / positive regulation of cytokinesis / G protein-coupled receptor activity / Sensory perception of sweet, bitter, and umami (glutamate) taste / G alpha (i) signalling events / receptor complex / G protein-coupled receptor signaling pathway / Golgi apparatus / membrane / plasma membrane
Similarity search - Function
GPCR, family 3, extracellular calcium-sensing receptor-related / G-protein coupled receptors family 3 signature 2. / GPCR, family 3, nine cysteines domain / GPCR, family 3, nine cysteines domain superfamily / Nine Cysteines Domain of family 3 GPCR / GPCR, family 3, conserved site / GPCR, family 3 / G-protein coupled receptors family 3 profile. / GPCR family 3, C-terminal / 7 transmembrane sweet-taste receptor of 3 GCPR ...GPCR, family 3, extracellular calcium-sensing receptor-related / G-protein coupled receptors family 3 signature 2. / GPCR, family 3, nine cysteines domain / GPCR, family 3, nine cysteines domain superfamily / Nine Cysteines Domain of family 3 GPCR / GPCR, family 3, conserved site / GPCR, family 3 / G-protein coupled receptors family 3 profile. / GPCR family 3, C-terminal / 7 transmembrane sweet-taste receptor of 3 GCPR / Receptor, ligand binding region / Receptor family ligand binding region / Periplasmic binding protein-like I
Similarity search - Domain/homology
Taste receptor type 1 member 3 / Taste receptor type 1 member 2
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.93 Å
AuthorsWang H / Chen X / Dai Y / Lee CH
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM143282 United States
CitationJournal: To Be Published
Title: Structure of the human sweet receptor bound to advantame in the intermediate state, extracellular domain
Authors: Wang H / Chen X / Dai Y / Lee CH
History
DepositionMay 20, 2025-
Header (metadata) releaseSep 3, 2025-
Map releaseSep 3, 2025-
UpdateSep 3, 2025-
Current statusSep 3, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_70740.png

Downloads & links

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Map

FileDownload / File: emd_70740.map.gz / Format: CCP4 / Size: 52.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.3 Å/pix.
x 240 pix.
= 311.52 Å		1.3 Å/pix.
x 240 pix.
= 311.52 Å		1.3 Å/pix.
x 240 pix.
= 311.52 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.298 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.686
Minimum - Maximum-1.2712379 - 2.5448139
Average (Standard dev.)0.0045788405 (±0.07309089)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions240240240
Spacing240240240
CellA=B=C: 311.52 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_70740_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_70740_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Human TAS1R2 and TAS1R3 sweet receptor bound to advantame

EntireName: Human TAS1R2 and TAS1R3 sweet receptor bound to advantame
Components
  • Complex: Human TAS1R2 and TAS1R3 sweet receptor bound to advantame
    • Protein or peptide: Taste receptor type 1 member 2
    • Protein or peptide: Taste receptor type 1 member 3
  • Ligand: advantame

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Supramolecule #1: Human TAS1R2 and TAS1R3 sweet receptor bound to advantame

SupramoleculeName: Human TAS1R2 and TAS1R3 sweet receptor bound to advantame
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Taste receptor type 1 member 2

MacromoleculeName: Taste receptor type 1 member 2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 92.842008 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: AENSDFYLPG DYLLGGLFSL HANMKGIVHL NFLQVPMCKE YEVKVIGYNL MQAMRFAVEE INNDSSLLPG VLLGYEIVDV CYISNNVQP VLYFLAHEDN LLPIQEDYSN YSSRVVAVIG PDNSESVMTV ANFLSLFLLP QITYSAISDE LRDKVRFPAL L RTTPSADH ...String:
AENSDFYLPG DYLLGGLFSL HANMKGIVHL NFLQVPMCKE YEVKVIGYNL MQAMRFAVEE INNDSSLLPG VLLGYEIVDV CYISNNVQP VLYFLAHEDN LLPIQEDYSN YSSRVVAVIG PDNSESVMTV ANFLSLFLLP QITYSAISDE LRDKVRFPAL L RTTPSADH HIEAMVQLML HFRWNWIIVL VSNDTYGRDN GQLLGERVAR RGICIAFQET LPTLQPNQNM TSEERQRLVT IV DKLQQST ARVVVVFSPD LTLYHFFNEV LRQNFTGAVW IASESWAIDP VLHNLTELRH LGTFLGITIQ SVPIPGFSEF REW GPQAGP PPLSRTSQSY TCNQECDNCL NATLSFNTIL RLSGERVVYS VYSAVYAVAH ALHSLLGCDK STCTKRVVYP WQLL EEIWK VNFTLLDHQI FFDPQGDVAL HLEIVQWQWD RSQNPFQSVA SYYPLQRQLK NIQDISWHTI NNTIPMSMCS KRCQS GQKK KPVGIHVCCF ECIDCLPGTF LNHTEDEYEC QACPNNEWSY QSETSCFKRQ LVFLEWHEAP TIAVALLAAL GFLSTL AIL VIFWRHFQTP IVRSAGGPMC FLMLTLLLVA YMVVPVYVGP PKVSTCLCRQ ALFPLCFTIC ISCIAVRSFQ IVCAFKM AS RFPRAYSYWV RYQGPYVSMA FITVLKMVIV VIGMLATGLS PTTRTDPDDP KITIVSCNPN YRNSLLFNTS LDLLLSVV G FSFAYMGKEL PTNYNEAKFI TLSMTFYFTS SVSLCTFMSA YSGVLVTIVD LLVTVLNLLA ISLGYFGPKC YMILFYPER NTPAYFNSMI QGYTMRRD

UniProtKB: Taste receptor type 1 member 2

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Macromolecule #2: Taste receptor type 1 member 3

MacromoleculeName: Taste receptor type 1 member 3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 91.485398 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: APLCLSQQLR MKGDYVLGGL FPLGEAEEAG LRSRTRPSSP VCTRFSSNGL LWALAMKMAV EEINNKSDLL PGLRLGYDLF DTCSEPVVA MKPSLMFLAK AGSRDIAAYC NYTQYQPRVL AVIGPHSSEL AMVTGKFFSF FLMPQVSYGA SMELLSARET F PSFFRTVP ...String:
APLCLSQQLR MKGDYVLGGL FPLGEAEEAG LRSRTRPSSP VCTRFSSNGL LWALAMKMAV EEINNKSDLL PGLRLGYDLF DTCSEPVVA MKPSLMFLAK AGSRDIAAYC NYTQYQPRVL AVIGPHSSEL AMVTGKFFSF FLMPQVSYGA SMELLSARET F PSFFRTVP SDRVQLTAAA ELLQEFGWNW VAALGSDDEY GRQGLSIFSA LAAARGICIA HEGLVPLPRA DDSRLGKVQD VL HQVNQSS VQVVLLFASV HAAHALFNYS ISSRLSPKVW VASEAWLTSD LVMGLPGMAQ MGTVLGFLQR GAQLHEFPQY VKT HLALAT DPAFCSALGE REQGLEEDVV GQRCPQCDCI TLQNVSAGLN HHQTFSVYAA VYSVAQALHN TLQCNASGCP AQDP VKPWQ LLENMYNLTF HVGGLPLRFD SSGNVDMEYD LKLWVWQGSV PRLHDVGRFN GSLRTERLKI RWHTSDNQKP VSRCS RQCQ EGQVRRVKGF HSCCYDCVDC EAGSYRQNPD DIACTFCGQD EWSPERSTRC FRRRSRFLAW GEPAVLLLLL LLSLAL GLV LAALGLFVHH RDSPLVQASG GPLACFGLVC LGLVCLSVLL FPGQPSPARC LAQQPLSHLP LTGCLSTLFL QAAEIFV ES ELPLSWADRL SGCLRGPWAW LVVLLAMLVE VALCTWYLVA FPPEVVTDWH MLPTEALVHC RTRSWVSFGL AHATNATL A FLCFLGTFLV RSQPGCYNRA RGLTFAMLAY FITWVSFVPL LANVQVVLRP AVQMGALLLC VLGILAAFHL PRCYLLMRQ PGLNTPEFFL GGGPGDAQGQ NDGNTGNQGK HE

UniProtKB: Taste receptor type 1 member 3

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Macromolecule #3: advantame

MacromoleculeName: advantame / type: ligand / ID: 3 / Number of copies: 1 / Formula: A1CD7
Molecular weightTheoretical: 458.504 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 59.3 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.1 µm / Nominal defocus min: 1.1 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.93 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 40562
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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