- EMDB-7071: Single particle cryo-EM structure determination of the LuIII caps... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-7071
タイトル
Single particle cryo-EM structure determination of the LuIII capsid protein
マップデータ
The capsid structure for the oncolytic parvovirus LuIII determined by single-particle electron microscopy to 3.17 Angstrom resolution
試料
ウイルス: LuIII virus (ウイルス)
タンパク質・ペプチド: Capsid protein VP2
キーワード
Parvoviridae / VP2 capsid protein / VIRUS LIKE PARTICLE
機能・相同性
機能・相同性情報
permeabilization of host organelle membrane involved in viral entry into host cell / symbiont entry into host cell via permeabilization of inner membrane / microtubule-dependent intracellular transport of viral material towards nucleus / T=1 icosahedral viral capsid / viral penetration into host nucleus / clathrin-dependent endocytosis of virus by host cell / host cell nucleus / virion attachment to host cell / structural molecule activity / metal ion binding 類似検索 - 分子機能
Phospholipase A2-like domain / Phospholipase A2-like domain / Parvovirus coat protein VP2 / Parvovirus coat protein VP1/VP2 / Parvovirus coat protein VP2 / Capsid/spike protein, ssDNA virus 類似検索 - ドメイン・相同性
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM082946-07S1, 229
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM082946
米国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
5T32AI007110-34
米国
National Institutes of Health/National Cancer Institute (NIH/NCI)
CA029303
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
U24GM116792
米国
引用
ジャーナル: Viruses / 年: 2017 タイトル: Atomic Resolution Structure of the Oncolytic Parvovirus LuIII by Electron Microscopy and 3D Image Reconstruction. 著者: Nikéa Pittman / Adam Misseldine / Lorena Geilen / Sujata Halder / J Kennon Smith / Justin Kurian / Paul Chipman / Mandy Janssen / Robert Mckenna / Timothy S Baker / Anthony D'Abramo / Susan ...著者: Nikéa Pittman / Adam Misseldine / Lorena Geilen / Sujata Halder / J Kennon Smith / Justin Kurian / Paul Chipman / Mandy Janssen / Robert Mckenna / Timothy S Baker / Anthony D'Abramo / Susan Cotmore / Peter Tattersall / Mavis Agbandje-McKenna / 要旨: LuIII, a protoparvovirus pathogenic to rodents, replicates in human mitotic cells, making it applicable for use to kill cancer cells. This virus group includes H-1 parvovirus (H-1PV) and minute virus ...LuIII, a protoparvovirus pathogenic to rodents, replicates in human mitotic cells, making it applicable for use to kill cancer cells. This virus group includes H-1 parvovirus (H-1PV) and minute virus of mice (MVM). However, LuIII displays enhanced oncolysis compared to H-1PV and MVM, a phenotype mapped to the major capsid viral protein 2 (VP2). This suggests that within LuIII VP2 are determinants for improved tumor lysis. To investigate this, the structure of the LuIII virus-like-particle was determined using single particle cryo-electron microscopy and image reconstruction to 3.17 Å resolution, and compared to the H-1PV and MVM structures. The LuIII VP2 structure, ordered from residue 37 to 587 (C-terminal), had the conserved VP topology and capsid morphology previously reported for other protoparvoviruses. This includes a core β-barrel and α-helix A, a depression at the icosahedral 2-fold and surrounding the 5-fold axes, and a single protrusion at the 3-fold axes. Comparative analysis identified surface loop differences among LuIII, H-1PV, and MVM at or close to the capsid 2- and 5-fold symmetry axes, and the shoulder of the 3-fold protrusions. The 2-fold differences cluster near the previously identified MVM sialic acid receptor binding pocket, and revealed potential determinants of protoparvovirus tumor tropism.