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データを開く
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基本情報
| 登録情報 | ![]() | |||||||||
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| タイトル | Membrane-associated human mitoribosome in complex with TACO1 | |||||||||
マップデータ | ||||||||||
試料 |
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キーワード | mitochondria / mitoribosome / inner membrane / RIBOSOME | |||||||||
| 機能・相同性 | 機能・相同性情報negative regulation of mitochondrial mRNA catabolic process / Mitochondrial mRNA modification / mitochondrial mRNA polyadenylation / Complex IV assembly / motor learning / Mitochondrial RNA degradation / mitochondrial protein quality control / negative regulation of oxidoreductase activity / mitochondrial ribosome binding / membrane insertase activity ...negative regulation of mitochondrial mRNA catabolic process / Mitochondrial mRNA modification / mitochondrial mRNA polyadenylation / Complex IV assembly / motor learning / Mitochondrial RNA degradation / mitochondrial protein quality control / negative regulation of oxidoreductase activity / mitochondrial ribosome binding / membrane insertase activity / protein insertion into mitochondrial inner membrane from matrix / mitochondrial RNA catabolic process / regulation of mitochondrial translation / mitochondrial respiratory chain complex IV assembly / Complex I biogenesis / negative regulation of ATP-dependent activity / rRNA import into mitochondrion / mitochondrial transcription / mitochondrial translational termination / mitochondrial translational elongation / mitochondrial ribosome assembly / translation release factor activity, codon nonspecific / Mitochondrial translation elongation / Mitochondrial translation initiation / mitochondrial proton-transporting ATP synthase complex assembly / flagellated sperm motility / Mitochondrial ribosome-associated quality control / Mitochondrial translation termination / peptidyl-tRNA hydrolase / mitochondrial large ribosomal subunit / mitochondrial ribosome / mitochondrial small ribosomal subunit / nuclear inner membrane / peptidyl-tRNA hydrolase activity / mitochondrial translation / mitochondrion transport along microtubule / positive regulation of proteolysis / apoptotic mitochondrial changes / nuclear outer membrane / mitochondrial nucleoid / sperm head-tail coupling apparatus / ribosomal small subunit binding / mitochondrial respiratory chain complex I assembly / spermatid development / anatomical structure morphogenesis / mRNA transport / single fertilization / sperm flagellum / RNA processing / beta-tubulin binding / Mitochondrial protein degradation / rescue of stalled cytosolic ribosome / acrosomal vesicle / aerobic respiration / cellular response to leukemia inhibitory factor / condensed nuclear chromosome / mRNA 3'-UTR binding / apoptotic signaling pathway / sperm end piece / mitochondrion organization / protein tetramerization / autophagy / mitochondrial membrane / fibrillar center / cell junction / regulation of translation / double-stranded RNA binding / single-stranded DNA binding / 5S rRNA binding / small ribosomal subunit / small ribosomal subunit rRNA binding / endonuclease activity / sperm midpiece / large ribosomal subunit rRNA binding / nuclear membrane / microtubule binding / 加水分解酵素; 酸無水物に作用; GTPに作用・細胞または細胞小器官の運動に関与 / cytoskeleton / microtubule / tRNA binding / cell population proliferation / mitochondrial inner membrane / negative regulation of translation / rRNA binding / nuclear body / structural constituent of ribosome / ribosome / translation / mitochondrial matrix / ribonucleoprotein complex / protein domain specific binding / nucleotide binding / hydrolase activity / mRNA binding / GTPase activity / apoptotic process / ubiquitin protein ligase binding / positive regulation of DNA-templated transcription / GTP binding / nucleolus 類似検索 - 分子機能 | |||||||||
| 生物種 | Homo sapiens (ヒト) | |||||||||
| 手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.46 Å | |||||||||
データ登録者 | Wang S / Xiong Y / Zhang Y | |||||||||
| 資金援助 | 米国, 1件
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引用 | ジャーナル: Nat Commun / 年: 2026タイトル: Structural basis of TACO1-mediated efficient mitochondrial translation. 著者: Shuhui Wang / Michele Brischigliaro / Yuekang Zhang / Chunxiang Wu / Wei Zheng / Antoni Barrientos / Yong Xiong / ![]() 要旨: Translation elongation is a universally conserved step in protein synthesis, relying on elongation factors that engage the ribosomal L7/L12 stalk to mediate aminoacyl-tRNA delivery, accommodation, ...Translation elongation is a universally conserved step in protein synthesis, relying on elongation factors that engage the ribosomal L7/L12 stalk to mediate aminoacyl-tRNA delivery, accommodation, and ribosomal translocation. Using in organello cryo-electron microscopy, we reveal how the mitochondrial translation accelerator TACO1 promotes efficient elongation on human mitoribosomes. TACO1 binds the mitoribosomal region typically bound by elongation factor Tu (mtEF-Tu), bridging the large and small subunits via contacts with 16S rRNA, bL12m, A-site tRNA, and uS12m. While active throughout elongation, TACO1 is especially critical when translating polyproline motifs. Its absence prolongs mtEF-Tu residence in A/T states, causes persistent mitoribosomal stalling and premature subunit dissociation. Structural analyses indicate that TACO1 competes with mtEF-Tu for mitoribosome binding, stabilizes A-site tRNA, and enhances peptidyl transfer through a mechanism distinct from EF-P and eIF5A. These findings suggest that bacterial TACO1 orthologs may serve analogous roles, highlighting an evolutionarily conserved strategy for maintaining elongation efficiency during challenging translation events. | |||||||||
| 履歴 |
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構造の表示
| 添付画像 |
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ダウンロードとリンク
-EMDBアーカイブ
| マップデータ | emd_70592.map.gz | 18.1 MB | EMDBマップデータ形式 | |
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| ヘッダ (付随情報) | emd-70592-v30.xml emd-70592.xml | 146 KB 146 KB | 表示 表示 | EMDBヘッダ |
| FSC (解像度算出) | emd_70592_fsc_1.xml emd_70592_fsc_2.xml emd_70592_fsc_3.xml | 15.7 KB 15.8 KB 15.7 KB | 表示 表示 表示 | FSCデータファイル |
| 画像 | emd_70592.png | 73.3 KB | ||
| マスクデータ | emd_70592_msk_1.map | 421.9 MB | マスクマップ | |
| Filedesc metadata | emd-70592.cif.gz | 25.5 KB | ||
| その他 | emd_70592_additional_1.map.gz emd_70592_additional_10.map.gz emd_70592_additional_2.map.gz emd_70592_additional_3.map.gz emd_70592_additional_4.map.gz emd_70592_additional_5.map.gz emd_70592_additional_6.map.gz emd_70592_additional_7.map.gz emd_70592_additional_8.map.gz emd_70592_additional_9.map.gz emd_70592_half_map_1.map.gz emd_70592_half_map_2.map.gz | 213.1 MB 3.3 MB 391.9 MB 391.9 MB 398.9 MB 1.1 MB 213.5 MB 391.9 MB 391.9 MB 398.9 MB 392 MB 392 MB | ||
| アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-70592 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-70592 | HTTPS FTP |
-関連構造データ
| 関連構造データ | ![]() 9olfMC ![]() 9pg8C ![]() 9pgfC ![]() 9pgiC ![]() 9pglC ![]() 9pgmC ![]() 9pr4C ![]() 9praC ![]() 9prdC ![]() 9prqC ![]() 9prxC ![]() 9ps0C ![]() 9ps7C ![]() 9psiC ![]() 9psmC C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
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| 類似構造データ | 類似検索 - 機能・相同性 F&H 検索 |
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リンク
| EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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| 「今月の分子」の関連する項目 |
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マップ
| ファイル | ダウンロード / ファイル: emd_70592.map.gz / 形式: CCP4 / 大きさ: 421.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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| 投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
| ボクセルのサイズ | X=Y=Z: 1.068 Å | ||||||||||||||||||||||||||||||||||||
| 密度 |
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| 対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
| 詳細 | EMDB XML:
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-添付データ
+マスク #1
+追加マップ: #10
+追加マップ: #1
+追加マップ: #9
+追加マップ: #8
+追加マップ: #7
+追加マップ: #6
+追加マップ: #5
+追加マップ: #4
+追加マップ: #3
+追加マップ: #2
+ハーフマップ: #2
+ハーフマップ: #1
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試料の構成要素
+全体 : mitochondria
+超分子 #1: mitochondria
+分子 #1: 39S ribosomal protein L32, mitochondrial
+分子 #2: 39S ribosomal protein L33, mitochondrial
+分子 #3: 39S ribosomal protein L34, mitochondrial
+分子 #4: 39S ribosomal protein L35, mitochondrial
+分子 #5: 39S ribosomal protein L36, mitochondrial
+分子 #6: 39S ribosomal protein L37, mitochondrial
+分子 #7: 39S ribosomal protein L38, mitochondrial
+分子 #8: 39S ribosomal protein L39, mitochondrial
+分子 #9: 39S ribosomal protein L40, mitochondrial
+分子 #10: 39S ribosomal protein L41, mitochondrial
+分子 #12: Small ribosomal subunit protein mS34
+分子 #13: 28S ribosomal protein S35, mitochondrial
+分子 #14: Small ribosomal subunit protein mS37
+分子 #15: Aurora kinase A-interacting protein
+分子 #16: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
+分子 #17: Leucine-rich PPR motif-containing protein, mitochondrial
+分子 #18: SRA stem-loop-interacting RNA-binding protein, mitochondrial
+分子 #20: 28S ribosomal protein S2, mitochondrial
+分子 #21: 28S ribosomal protein S24, mitochondrial
+分子 #22: 28S ribosomal protein S5, mitochondrial
+分子 #23: 28S ribosomal protein S6, mitochondrial
+分子 #24: 28S ribosomal protein S7, mitochondrial
+分子 #25: 28S ribosomal protein S9, mitochondrial
+分子 #26: 28S ribosomal protein S10, mitochondrial
+分子 #27: 28S ribosomal protein S11, mitochondrial
+分子 #28: 28S ribosomal protein S12, mitochondrial
+分子 #29: 28S ribosomal protein S14, mitochondrial
+分子 #30: 28S ribosomal protein S15, mitochondrial
+分子 #31: 28S ribosomal protein S16, mitochondrial
+分子 #32: 28S ribosomal protein S17, mitochondrial
+分子 #33: 28S ribosomal protein S18b, mitochondrial
+分子 #34: 28S ribosomal protein S18c, mitochondrial
+分子 #35: Small ribosomal subunit protein bS21m
+分子 #36: 28S ribosomal protein S22, mitochondrial
+分子 #37: 28S ribosomal protein S23, mitochondrial
+分子 #38: 28S ribosomal protein S25, mitochondrial
+分子 #39: 28S ribosomal protein S26, mitochondrial
+分子 #40: 28S ribosomal protein S27, mitochondrial
+分子 #41: 28S ribosomal protein S28, mitochondrial
+分子 #42: 28S ribosomal protein S29, mitochondrial
+分子 #43: 28S ribosomal protein S31, mitochondrial
+分子 #44: 28S ribosomal protein S33, mitochondrial
+分子 #50: Translational activator of cytochrome c oxidase 1
+分子 #51: 39S ribosomal protein L2, mitochondrial
+分子 #52: 39S ribosomal protein L3, mitochondrial
+分子 #53: 39S ribosomal protein L4, mitochondrial
+分子 #54: 39S ribosomal protein L12, mitochondrial
+分子 #55: 39S ribosomal protein L9, mitochondrial
+分子 #56: 39S ribosomal protein L10, mitochondrial
+分子 #57: 39S ribosomal protein L11, mitochondrial
+分子 #58: Large ribosomal subunit protein uL13m
+分子 #59: 39S ribosomal protein L14, mitochondrial
+分子 #60: 39S ribosomal protein L15, mitochondrial
+分子 #61: 39S ribosomal protein L16, mitochondrial
+分子 #62: 39S ribosomal protein L17, mitochondrial
+分子 #63: Mitochondrial inner membrane protein OXA1L
+分子 #64: 39S ribosomal protein L18, mitochondrial
+分子 #65: 39S ribosomal protein L19, mitochondrial
+分子 #66: 39S ribosomal protein L20, mitochondrial
+分子 #67: 39S ribosomal protein L21, mitochondrial
+分子 #68: 39S ribosomal protein L22, mitochondrial
+分子 #69: 39S ribosomal protein L23, mitochondrial
+分子 #70: 39S ribosomal protein L24, mitochondrial
+分子 #71: 39S ribosomal protein L27, mitochondrial
+分子 #72: 39S ribosomal protein L28, mitochondrial
+分子 #73: 39S ribosomal protein L47, mitochondrial
+分子 #74: 39S ribosomal protein L30, mitochondrial
+分子 #75: 39S ribosomal protein L42, mitochondrial
+分子 #76: 39S ribosomal protein L43, mitochondrial
+分子 #77: 39S ribosomal protein L44, mitochondrial
+分子 #78: 39S ribosomal protein L45, mitochondrial
+分子 #79: 39S ribosomal protein L46, mitochondrial
+分子 #80: 39S ribosomal protein L48, mitochondrial
+分子 #81: 39S ribosomal protein L49, mitochondrial
+分子 #82: 39S ribosomal protein L50, mitochondrial
+分子 #83: 39S ribosomal protein L51, mitochondrial
+分子 #84: 39S ribosomal protein L52, mitochondrial
+分子 #85: Large ribosomal subunit protein mL53
+分子 #86: 39S ribosomal protein L54, mitochondrial
+分子 #87: 39S ribosomal protein L55, mitochondrial
+分子 #88: Nascent polypeptide
+分子 #89: Ribosomal protein 63, mitochondrial
+分子 #90: Peptidyl-tRNA hydrolase ICT1, mitochondrial
+分子 #91: Growth arrest and DNA damage-inducible proteins-interacting protein 1
+分子 #92: 39S ribosomal protein S18a, mitochondrial
+分子 #93: 39S ribosomal protein S30, mitochondrial
+分子 #94: Large ribosomal subunit protein uL1m
+分子 #11: 16S mitochondrial rRNA
+分子 #19: 12S mitochondrial rRNA
+分子 #45: A/A-tRNA
+分子 #46: P/P-tRNA
+分子 #47: E/E-tRNA
+分子 #48: mRNA
+分子 #49: RNA (73-MER)mitochondrial tRNAVal
+分子 #95: ZINC ION
+分子 #96: SPERMIDINE
+分子 #97: MAGNESIUM ION
+分子 #98: 1,4-DIAMINOBUTANE
+分子 #99: POTASSIUM ION
+分子 #100: SPERMINE
+分子 #101: NICOTINAMIDE-ADENINE-DINUCLEOTIDE
+分子 #102: FE2/S2 (INORGANIC) CLUSTER
+分子 #103: GUANOSINE-5'-DIPHOSPHATE
+分子 #104: ADENOSINE-5'-TRIPHOSPHATE
+分子 #105: VALINE
-実験情報
-構造解析
| 手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
| 試料の集合状態 | particle |
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試料調製
| 緩衝液 | pH: 7.4 |
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| 凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
| 顕微鏡 | TFS KRIOS |
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| 撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 55.0 e/Å2 |
| 電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
| 電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 4.0 µm / 最小 デフォーカス(公称値): 1.0 µm |
| 実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
ムービー
コントローラー
万見について




キーワード
Homo sapiens (ヒト)
データ登録者
米国, 1件
引用


















































Z (Sec.)
Y (Row.)
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解析
FIELD EMISSION GUN


